Book Cover
Author Schepers, Ute.

Title RNA interference in practice / Ute Schepers
Published Weinheim : Wiley-VCH, [2005]
Online access available from:
Wiley Online Books    View Resource Record  


Description 1 online resource (x, 326 pages) : illustrations
Series Wiley InterScience online books
Contents 1. Introduction : RNA interference, the "breakthrough of the year 2002" -- 2. RNAi in Caenorhabditis elegans -- 3. RNAi in Drosophila -- 4. RNAi in mammals -- App. Suppliers of RNAi-related chemicals and probes
Summary This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn: How RNAi works in worms, flies and mammals; How to design the most efficient RNAi constructs; How to achieve transient, stable and conditional RNAi in cell cultures; How to determine the efficiency of an RNAi experiment; and how to use RNAi for gene therapy. All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiment
Bibliography Includes bibliographical references and index
Notes Print version record
Subject RNA editing.
RNA Interference.
Form Electronic book
ISBN 3527310207
3527604375 (electronic bk.)
9783527604371 (electronic bk.)