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E-book

Title Standards of mouse model phenotyping / edited by Martin Hrabé de Angelis, Pierre Chambon, and Steve Brown
Published Weinheim : Wiley-VCH, [2006]
©2006
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Description 1 online resource (xxvi, 350 pages) : illustrations
Contents Standards of Mouse Model Phenotyping; Foreword; Table of Contents; Preface; 1 Characterizing Hearing in Mice; 1.1 Introduction; 1.2 Behavioral Tests of Hearing; 1.3 Physiological Tests of Hearing; 1.4 Anatomy of the Ear; 1.5 Conclusions; Acknowledgements; 2 Molecular Phenotyping: Gene Expression Profiling; 2.1 Why this Screen? Medical and Biological Relevance; 2.2 Examples: Diseases of Mouse and Man; 2.3 Diagnostic Methods: History and State of the Art; 2.4 Technical Requirements for Screening Protocols (Short): First and Second Line Approaches; 2.5 Logistics (Whom, When, How Many, Why)
2.5.1 Choice of Platform2.5.2 Biological Samples; 2.6 Trouble Shooting; 2.6.1 Preparation of Hybridization Target; 2.6.2 Critical Issues of Chip Hybridization; 2.6.3 Image Processing and Array Design; 2.7 Short-term Outlook; 3 Screening for Bone and Cartilage Phenotypes in Mice; 3.1 Introduction; 3.1.1 The Skeleton; 3.1.2 Skeletal Development in the Embryo; 3.1.3 Growth and Maintenance of Bone and Cartilage; 3.1.4 Diseases Involving Cartilage and Bone; 3.1.5 The Mouse as a Model for Skeletal Diseases; 3.2 Screening Protocols; 3.2.1 Morphological Analysis; 3.2.1.1 Protocol
3.2.2 X-Ray Analysis3.2.2.1 General; 3.2.2.2 Imaging; 3.2.2.3 X-Ray Analysis; 3.2.2.4 Protocol; 3.2.3 DXA-Analysis; 3.2.3.1 General; 3.2.3.2 Advantages; 3.2.3.3 Disadvantages; 3.2.3.4 Small Animal Applications; 3.2.3.5 Precision and Accuracy; 3.2.3.6 Considerations; 3.2.3.7 Protocol; 3.2.4 Biochemical Bone Markers; 3.2.4.1 Clinical Utility of Biochemical Markers of Bone Turnover in Small Animals; 3.2.4.2 Mouse Markers of Bone Turnover/Metabolism and Hormonal Regulation; 3.2.4.3 Variability/Sensitivity/Sample Choice; 3.2.4.4 Which Markers Should be Used During the Screen?
4.2.2 Clinical Chemistry in Selected Disorders4.2.2.1 Hypercholesterolemia; 4.2.2.2 Albuminuria; 4.2.2.3 Acute Myeloid Leukemia (AML); 4.3 Clinical Chemistry as Diagnostic Tool; 4.3.1 History; 4.3.2 State of the Art; 4.4 Technical Requirements and Screening Protocols; 4.4.1 Technical Requirements; 4.4.1.1 Blood Collection; 4.4.1.2 Sample Preparation; 4.4.1.3 Sample Analysis; 4.4.2 Screening Protocols; 4.4.2.1 Primary Screen; 4.4.2.2 Secondary Screen; 4.4.2.3 Tertiary Screen; 4.5 Logistics of the Screen; 4.5.1 General Considerations; 4.5.2 Lessons from ENU Mutants; 4.6 Trouble Shooting
Summary This is the first book in the field of mouse genetics to provide comprehensive and standardized methods for the characterization of laboratory mice. The editor is Director of the German Mouse Clinic and member of the Project Committee of the German National Genome Research Network and provides here a brief introduction to the mouse as a model for diseases and functional analysis of genes and proteins. Throughout, he focuses on the characterization of mouse models using the latest phenotyping methods, with the different areas presented in a clearly structured and easily accessible manner
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL
Print version record
Subject Mice as laboratory animals.
Mice -- Genetics.
Phenotype.
Transgenic mice.
Disease Models, Animal.
Mice -- genetics.
Mice, Transgenic -- genetics.
Phenotype.
Form Electronic book
Author Brown, Stephen D. M.
Chambon, Pierre.
Hrabé de Angelis, Martin.
Wiley InterScience (Online service)
ISBN 1282302442
3527608702 (electronic bk.)
3527611940
6612302445
9781282302440
9783527608706 (electronic bk.)
9783527611942
9786612302442
(cased)
(alk. paper)