| Description |
387 pages : illustrations some color ; 24 cm |
| Series |
Springer lab manual |
|
Springer lab manual.
|
| Contents |
Pt. I. Molecular Biology Techniques -- Ch. 1. Structure and Function of the Human Genome / Friedhelm Hildebrandt -- Ch. 2. Enzymatic Modification of DNA and RNA / Kai-Olaf Netzer -- Ch. 3. Purification and Nucleic Acids From Eukaryotic Cells / Hans Gerd Nothwang and Friedhelm Hildebrandt -- Ch. 4. Electrophoresis of DNA and RNA Fragments / Feng Qian and Gregory G. Germino -- Ch. 5. Single-Strand Conformation Polymorphism (SSCP) Analysis / Feng Qian and Gregory G. Germino -- Ch. 6. Denaturing Gradient Gel Electrophoresis / Kai-Olaf Netzer -- Ch. 7. Introduction of Pulsed-Field Gel Electrophoresis (PFGE) / Feng Qian and Gregory G. Germino -- Ch. 8. Pulsed-Field Gel Electrophoresis: Protocols / Gudrun A. Rappold, Karin Ried and Albrecht Klink / [et al.] -- Ch. 9. Radioactive Labeling of DNA and RNA Probes / Peter Igarashi -- Ch. 10. Hybridization Methods (Southern and Northern Blotting) / Kai-Olaf Netzer -- Ch. 11. Specimen Preparation Techniques for Cytogenetic Analysis by Fluorescence in situ Hybridization / Stefan Joos, Martin Bentz and Anton H. N. Hopman / [et al.] -- Ch. 12. Chromosome Analysis by Fluorescence in situ Hybridization / Martin Bentz, Stefan Joos and Peter Lichter -- Ch. 13. Sequencing / Klaus Deichmann -- Ch. 14. Polymerase Chain Reaction / Friedhelm Hildebrandt and Iva Singh-Sawhney -- Ch. 15. Cloning Vectors / Annette Deichmann and Klaus Deichmann -- Pt. II. Strategies for Gene Isolation and Characterization -- Ch. 16. Cloning Strategies: An Overview / Friedhelm Hildebrandt -- Ch. 17. Genomic Libraries / Luitz F. Onuchic and Gregory G. Germino -- Ch. 18. cDNA Libraries / Luitz F. Onuchic and Gregory G. Germino -- Ch. 19. cDNA Library Screening / Johann Peter Hossle -- Ch. 20. Expression Cloning with Xenopus Oocytes / Daniel Markovich, Andreas Werner and Heini Murer -- Ch. 21. Transgenic Animals / Eckart Schott, Martin Paul and Detlev Ganten -- Ch. 22. Expression in Eukaryotic Cells: Gene Transfer Using Retroviral Vectors / Martin Marx -- Ch. 23. Positional Cloning and Linkage Analysis / Friedhelm Hildebrandt and Heymut Omran -- Pt. III. Protein Methods -- Ch. 24. Protein Techniques / Robert F. Reilly |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Molecular biology -- Laboratory manuals.
|
|
Molecular diagnosis -- Laboratory manuals.
|
|
Medical genetics -- Laboratory manuals.
|
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Molecular Biology -- methods.
|
| Genre/Form |
Laboratory manuals.
|
| Author |
Hildebrandt, Friedhelm, 1957-
|
|
Igarashi, Peter.
|
| LC no. |
99031852 |
| ISBN |
3540571299 |
|
