| Description |
1 online resource (xi, 317 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; 1610 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1610. 1064-3745
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| Contents |
CRISPR/Cas-mediated in planta gene targeting / Simon Schiml, Friedrich Fauser, and Holger Puchta -- User guide for the LORE1 insertion mutant resource / Terry Mun, Anna Małolepszy, Niels Sandal, Jens Stougaard, and Stig U. Andersen -- Enabling reverse genetics in Medicago truncatula using high-throughput sequencing for Tnt1 flanking sequence recovery / Xiaofei Cheng, Nick Krom, Shulan Zhang, Kirankumar S. Mysore, Michael Udvardi, and Jiangqi Wen -- Generation of doubled haploid lines for QTL mapping / Daniele L. Filiault, Danelle K. Seymour, Ravi Maruthachalam, and Julin N. Maloof -- Assessing distribution and variation of genome-wide DNA methylation using short-read sequencing / Jörg Hagmann and Claude Becker -- Circular chromosome conformation capture in plants / Stefan Grob -- Genome-wide profiling of histone modifications and histone variants in Arabidopsis thaliana and Marchantia polymorpha / Ramesh Yelagandula, Akihisa Osakabe, Elin Axelsson, Frederic Berger, and Tomokazu Kawashima -- Tissue-specific transcriptome profiling in Arabidopsis roots / Erin E. Sparks and Philip N. Benfey -- Sample preparation protocols for protein abundance, acetylome, and phosphoproteome profiling of plant tissues / Gaoyuan Song, Maxwell R. McReynolds, and Justin W. Walley -- Automated high-throughput root phenotyping of Arabidopsis thaliana under nutrient deficiency conditions / Santosh B. Satbhai, Christian Göschl, and Wolfgang Busch -- Large-scale phenotyping of root traits in the model legume Lotus japonicus / Marco Giovannetti, Anna Małolepszy, Christian Göschl, and Wolfgang Busch -- Long-term confocal imaging of Arabidopsis thaliana roots for simultaneous quantification of root growth and fluorescent signals / Delyana Stoeva, Christian Göschl, Bruce Corliss, and Wolfgang Busch -- Identification of protein-DNA interactions using enhanced yeast one-hybrid assays and a semiautomated approach / Allison Gaudinier, Michelle Tang, Anne-Maarit Bågman, and Siobhan M. Brady -- Mapping protein-protein interaction using high-throughput yeast 2-Hybrid / Jessica Lopez and M. Shahid Mukhtar -- Mapping protein-protein interactions using affinity purification and mass spectrometry / Chin-Mei Lee, Christopher Adamchek, Ann Feke, Dmitri A. Nusinow, and Joshua M. Gendron -- Measuring protein movement, oligomerization state, and protein-protein interaction in Arabidopsis roots using scanning fluorescence correlation Spectroscopy (Scanning FCS) / Natalie M. Clark and Rosangela Sozzani -- Studying protein-protein interactions In Planta using advanced fluorescence microscopy / Marc Somssich and Rüdiger Simon -- Chemiluminescence-based detection of peptide activity and peptide-receptor binding in plants / Mari Wildhagen, Markus Albert, and Melinka A. Butenko -- Application of chemical genomics to plant-bacteria communication : a high-throughput system to identify novel molecules modulating the induction of bacterial virulence genes by plant signals / Elodie Vandelle, Maria Rita Puttilli, Andrea Chini, Giulia Devescovi, Vittorio Venturi, and Annalisa Polverari |
| Summary |
This volume provides protocols that revolve around three pillars of progress in the plant genomics field: genotypes, phenotypes, and the molecular processes in between. Chapters in Plant Genomics: Methods and Protocols are not restricted to the predominant model species Arabidopsis thaliana, hoping to encourage and facilitate other researchers to expand their research to other species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Genomics: Methods and Protocols aims to serve as an inspiration for further studies in plant genomics |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title form PDF title page (SpringerProtocols, viewed April 25, 2017) |
| Subject |
Plant genomes.
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Plant molecular genetics.
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Plant genomes -- Research -- Methodology
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Plant molecular genetics -- Research -- Methodology
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Genome, Plant
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Genomics -- methods
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Plants -- genetics
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Botany & plant sciences.
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Science -- Life Sciences -- Botany.
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Plant genomes
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Plant molecular genetics
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Busch, Wolfgang (Botanist), editor
|
| ISBN |
9781493970032 |
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1493970038 |
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