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Title Somatic stem cells : methods and protocols / edited by Shree Ram Singh
Published New York : Humana Press, ©2012

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Description 1 online resource (xvi, 546 pages)
Series Methods in molecular biology, 1064-3745 ; 879
Methods in molecular biology ; 879.
Contents Current thoughts on the therapeutic potential of stem cell / Pranela Rameshwar -- Unique FACS method to isolate stem cells in planarian / Tetsutaro Hayashi and Kiyokazu Agata -- Identification of neural stem cells in the Drosophila larval brain / Mo Weng, Hideyuki Komori, and Cheng-Yu Lee -- Generation and staining of intestinal stem cell lineage in adult midgut / Shree Ram Singh [and others] -- Developing a quantitative in vivo tissue reconstitution assay to assess the relative potency of candidate populations of mouse oesophageal epithelial cells / Daniel Croagh [and others] -- Identification, isolation, and culture of intestinal epithelial stem cells from murine intestine / A.D. Gracz, B.J. Puthoff, and S.T. Magness -- Isolation and characterization of distal lung progenitor cells / Barbara Driscoll [and others] -- Transplantation of mouse fetal liver cells for analyzing the function of hematopoietic stem and progenitor cells / Kristbjorn Orri Gudmundsson, Steven W. Stull, and Jonathan R. Keller -- Convenient and efficient enrichment of the CD133+ liver cells from rat fetal liver as a source of liver stem/progenitor cells / Weihui Liu, Nan You, and Kefeng Dou -- Assessing the potential clinical utility of transplantations of neural and mesenchymal stem cells for treating neurodegenerative diseases / Laurent Lescaudron [and others] -- Functional identification of neural stem cell-derived oligodendrocytes / Sofia Grade [and others] -- Stem/progenitor cells in murine mammary gland : isolation and functional characterization / Abhik Bandyopadhyay, Qiaoxiang Dong, and Lu-Zhe Sun -- Reporter assay to detect transfer and targeting of miRNAs in stem cell-breast cancer co-cultures / Steven J. Greco, Shyam A. Patel, and Pranela Rameshwar -- Isolation, culture, and osteogenic/chondrogenic differentiation of bone marrow-derived mesenchymal stem cells / Susanne Grassel, Sabine Stockl, and Zsuzsa Jenei-Lanzl -- Obtaining freshly isolated and cultured mesenchymal stem cells from human adipose tissue / Andrew C. Boquest and Philippe Collas -- Collection, processing, and banking of umbilical cord blood stem cells for transplantation and regenerative medicine / Michael S. Badowski and David T. Harris -- Generation of functional islets from human umbilical cord and placenta derived mesenchymal stem cells / Sachin Kadam, Vijayendran Govindasamy, and Ramesh Bhonde -- Isolation and characterization of human prostate Stem/Progenitor Cells / Changyong Guo, Baohui Zhang, and Isla P. Garraway -- Isolation and expansion of adult cardiac stem/progenitor cells in the form of cardiospheres from human cardiac biopsies and murine hearts / Isotta Chimenti [and others] -- Isolation and differentiation of human cardiomyocyte progenitor cells into cardiomyocytes / Anke M. Smits, Angelique A. van Oorschot, and Marie-Jose Goumans -- Isolation, expansion, and characterization of human islet-derived progenitor cells / Mugdha V. Joglekar and Anandwardhan A. Hardikar -- Isolation and characterization of resident mesenchymal stem cells in human glomeruli / Stefania Bruno and Giovanni Camussi -- Endothelial colony-forming progenitor cell isolation and expansion / Nicole A. Hofmann, Andreas Reinisch, and Dirk Strunk -- Isolation and characterization of stem cell-enriched human and canine hair follicle keratinocytes / Manabu Ohyama and Tetsuro Kobayashi -- Human salivary gland stem cells : isolation, propagation, and characterization / Silke Schwarz and Nicole Rotter -- Identification, isolation, characterization, and banking of human dental pulp stem cells / Virginia Tirino [and others] -- Isolation and differentiation potential of fibroblast-like stromal cells derived from human skin / Hsing-I Huang and Chung-Zu Wu -- Immortalization of human mesenchymal stromal cells with telomerase and red fluorescence protein expression / Chao-Ling Yao and Shiaw-Min Hwang -- Genetic modification of mesenchymal stem cells / Andréia Escosteguy Vargas [and others] -- Methodology, biology and clinical applications of human mesenchymal stem cells / Melissa Camassola [and others] -- In vitro production of enucleated red blood cells from hematopoietic stem and progenitor cells / Kenichi Miharada and Yukio Nakamura -- Methods for cancer stem cell detection and isolation / Virginia Tirino [and others] -- Biocompatible nanoparticle labeling of stem cells and their distribution in brain / Ashish K. Rehni [and others]
Summary Stem cells are found in almost all organisms from the early stages of development to the end of life. There are several types of stem cells and all of them may prove useful for medical research; however, each of the different types has both promise and limitations. Somatic Stem Cells: Methods and Protocols presents selected genetic, molecular, and cellular techniques used in somatic stem cell research and its clinical application. Chapters focus on the isolation, characterization, purity, plasticity, and clinical uses of somatic stem cells from a variety of human and animal tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Through and intuitive, Somatic Stem Cells: Methods and Protocols seeks to provides scientists with the fundamental techniques of stem cell research and its potential application in regenerative medicine
Analysis Life sciences
Cytology
Stem cells
Cell Biology
stamcellen
celbiologie
cellular biology
Cellular Biology
Celbiologie
Notes Includes index
Bibliography Includes bibliographical reference and index
Notes English
Subject Stem cells -- Laboratory manuals
Cells.
Cultures (Biology)
Medical laboratory technology.
Medical personnel.
Research.
Anatomy.
Science.
Physical sciences.
Stem cells.
Mesenchymal stem cells.
Regenerative medicine.
Cells
Investigative Techniques
Culture Techniques
Medicine
Publication Formats
Biomedical Research
Clinical Laboratory Techniques
Publication Characteristics
Health Occupations
Research
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Anatomy
Science
Disciplines and Occupations
Natural Science Disciplines
Stem Cell Research
Cell Culture Techniques
Stem Cells
Mesenchymal Stem Cells
Regenerative Medicine
Laboratory Manuals
Health Personnel
Health Workforce
Animal Structures
research (function)
anatomy.
sciences (philosophy)
physical sciences.
science (modern discipline)
Science
Research
Regenerative medicine
Physical sciences
Mesenchymal stem cells
Medical personnel
Medical laboratory technology
Cultures (Biology)
Cells
Anatomy
Stem cells
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Singh, Shree Ram
ISBN 9781617798153
1617798150
1617798142
9781617798146