| Description |
1 online resource (xv, 626 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 923 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 923. 1064-3745
|
| Contents |
In vitro culturing Plasmodium falciparum erythrocytic stages / Alexander G. Maier and Melanie Rug -- Production of Plasmodium falciparum gametocytes in vitro / Kathryn Shaw Saliba and Marcelo Jacobs-Lorena -- In vitro differentiation of Plasmodium falciparum gametocytes into ookinetes / Anil K. Ghosh and Marcelo Jacobs-Lorena -- Ex vivo culture of Plasmodium Vivax and Plasmodium Cynomolgi and in vitro culture of Plasmodium Knowlesi blood stages / Anne-Marie Zeeman, Annemarie Voorberg-van der Wel, and Clemens H.M. Kocken -- Laboratory maintenance of rodent malaria parasites / Chandra Ramakrishnan [and others] -- Transfection of Plasmodium falciparum / Melanie Rug and Alexander G. Maier -- Transfection of rodent malaria parasites / Nisha Philip, Rachael Orr, and Andrew P. Waters -- Recombination-mediated genetic engineering of Plasmodium berghei DNA / Claudia Pfander [and others] -- Standardization in generating and reporting genetically modified rodent malaria parasites : the RMgmDB database / Shahid M. Khan [and others] -- Transient transfection of Plasmodium vivax blood-stage parasi / Cecilia P. Sanchez [and others] -- Silencing of genes and alleles by RNAi in Anopheles gambiae / Marina Lamacchia [and others] -- Analysis of variant gene family expression by quantitative PCR / Sohini Sanyal, Thomas J. Templeton, and Cristina K. Moreira -- DNA microarray-based genome-wide analyses of Plasmodium parasites / Zbynek Bozdech, Sachel Mok, and Archna P. Gupta -- Whole-genome analysis of Plasmodium spp. utilizing a new agilent technologies DNA microarray platform / Heather J. Painter [and others] -- Transcriptome analysis using RNA-seq / Wieteke A.M. Hoeijmakers, Richárd Bártfai, and Hendrik G. Stunnenberg -- Experimental tools for the study of protein phosphorylation in Plasmodium / Dominique Dorin-Semblat [and others] -- Extraction of hydrophilic metabolites from Plasmodium falciparum-infected erythrocytes for metabolomic analysis / Kellen L. Olszewski and Manuel Llinás -- Static and dynamic imaging of erythrocyte invasion and early intra-erythrocytic development in Plasmodium falciparum / David T. Riglar and Jake Baum -- Flow cytometry-based methods for measurement of cytosolic calcium and surface protein expression in Plasmodium falciparum merozoites / Shailja Singh and Chetan E. Chitnis -- Microsphiltration : a microsphere matrix to explore erythrocyte deformability / Catherine Lavazec [and others] -- Atomic force microscopy of Plasmodium-infected red blood cells : detecting and localizing single molecular recognition events / Ang Li [and others] -- Expressing full-length functional PfEMP1 proteins in the HEK293 expression system / Anand Srivastava, Yves Durocher, and Benoît Gamain -- Genome-wide chromatin immunoprecipitation-sequencing in Plasmodium / Jose-Juan Lopez-Rubio, T. Nicolai Siegel, and Artur Scherf -- In situ fluorescence visualization of transcription sites and genomic loci in blood stages of Plasmodium falciparum / Liliana Mancio-Silva and Artur Scherf -- Bioluminescence imaging of P. berghei schizont sequestration in rodents / Joanna Braks [and others] -- Scoring sporozoite motility / Allison F. Carey, Robert Ménard, and Daniel Y. Bargieri -- Quantification of sporozoite invasion, migration, and development by microscopy and flow cytometry / Photini Sinnis [and others] -- Imaging sporozoite cell traversal in the liver of mice / Joana Tavares [and others] -- Analysis of liver stage development in and merozoite release from hepatocytes / Rebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler -- Quantitative analysis of Plasmodium berghei liver stages by Bioluminescence Imaging / Takeshi Annoura [and others] -- Activation of human NK cells by Plasmodium-Infected Red Blood Cells / Amir Horowitz and Eleanor M. Riley -- Chimeric Parasites as Tools to Study Plasmodium Immunology and Assess Malaria Vaccines / Ian Cockburn -- Development and Use of TCR Transgenic Mice for Malaria Immunology Research / Yun-Chi Chen and Fidel Zavala -- Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination / Katherine L. Doll, Noah S. Butler, and John T. Harty -- Screening Inhibitors of P. berghei Blood Stages Using Bioluminescent Reporter Parasites / Jing-wen Lin [and others] -- Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion Using Cytometry / Anthony Bouillon [and others] -- Induction of Anti-Plasmodium Immunity Following Subpatent Infection with Live Erythrocytic Stages and Drug Cure / Danielle I. Stanisic and Michael F. Good -- Vaccination Using Radiation- or Genetically Attenuated Live Sporozoites / Ashley M. Vaughan and Stefan H.I. Kappe -- Vaccination Using Normal Live Sporozoites Under Drug Treatment / Laurent Rénia [and others] -- Assessing Transmission Blockade in Plasmodium spp / Andrew M. Blagborough [and others] -- Mosquito Transgenic Technologies to Reduce Plasmodium Transmission / Silke Fuchs, Tony Nolan, and Andrea Crisanti |
| Summary |
Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques, genome manipulation methods, "omic" approaches, and techniques for studying the biology of the red blood cell and pre-erythrocytic stages of Plasmodium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Malaria: Methods and Protocols, Second Edition offers a comprehensive set of standard techniques for laboratory researchers |
| Analysis |
plasmodium falciparum |
|
malaria |
|
geneeskunde |
|
medicine |
|
volksgezondheid |
|
public health |
|
parasitologie |
|
parasitology |
|
infectieziekten |
|
infectious diseases |
|
laboratoriummethoden |
|
laboratory methods |
|
protocollen |
|
protocols |
|
Medicine (General) |
|
Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Online resource; title from PDF title page (Springer Protocols, viewed Oct. 30, 2012) |
| Subject |
Malaria -- Laboratory manuals
|
|
Malaria.
|
|
Malaria
|
|
Malaria
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Ménard, Robert (Researcher on malaria)
|
| ISBN |
9781627030267 |
|
1627030263 |
|
