Title Therapeutic proteins : methods and protocols / edited by Vladimir Voynov, Justin A. Caravella

Edition 2nd ed Published New York : Humana Press : Springer, ©2012

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Description 1 online resource (xi, 502 pages) : illustrations (some color) Series Methods in molecular biology, 1940-6029 ; 899 Methods in molecular biology (Clifton, N.J.) ; v. 899. 1064-3745 Contents Therapeutic proteins / Dimiter S. Dimitrov -- Synthetic antibody libraries / Bryce Nelson and Sachdev S. Sidhu -- Construction of "phylomer" peptide libraries as a rich source of potent inhibitors of protein/protein interactions / Nadia Milech and Paul Watt -- Ribosome display and screening for protein therapeutics / Damjana Kastelic and Mingyue He -- Yeast display of engineered antibody domains / Qi Zhao, Zhongyu Zhu, and Dimiter S. Dimitrov -- Expression, purification, and characterization of engineered antibody CH2 and VH domains / Rui Gong, Weizao Chen, and Dimiter S. Dimitrov -- Engineering of affibody molecules for therapy and diagnostics / Joachim Feldwisch and Vladimir Tolmachev -- Protein design for diversity of sequences and conformations using dead-end elimination / Karl J.M. Hanf -- Design and generation of DVD-Ig(TM) molecules for dual-specific targetingc/ Enrico DiGiammarino, Tariq Ghayur, and Junjian Liu -- Engineering and expression of bibody and tribody constructs in mammalian cells and in the Yeast Pichia pastoris / Steve Schoonooghe -- Use of E. coli for the production of a single protein / Lili Mao and Masayori Inouye -- Folding engineering strategies for effipient membrane protein production in E. coli / Brent L. Nannenga and Francois Baneyx -- Transient expression technologies : past, present, and future / Sabine Geisse and Bernd Voedisch -- Stable transfection pools for large quantity of protein production / Jianxin Ye -- Mammalian stable expression of biotherapeutics / Thomas Jostock and Hans-Peter Knopf -- Transgenic expression of therapeutic proteins in Arabidopsis thaliana seed / Cory L. Nykiforuk and Joseph G. Boothe -- Methods for chromatographic removal of endotoxin / Adam J. Lowe, Cameron L. Bardliving, and Carl A. Batt -- Effectiveness of various processing steps for viral clearance of therapeutic proteins : database analyses of commonly used steps / Dana Cipriano, Michael Burnham, and Joseph V. Hughes -- High-throughput quantitative N-glycan analysis of glycoproteins / Margaret Doherty, Ciara A. McManus, Rebecca Duke, and Pauline M. Rudd -- High-throughput multimodal strong anion exchange purification and N-glycan characterization of endogenous glycoprotein expressed in glycoengineered Pichia pastoris / Sujatha Gomathinayagam, Erik Hoyt, Alissa M. Thompson, Eric Brown, Khanita Karaveg, Stephen R. Hamilton, and Huijuan Li -- Databases and tools in glycobiology / Natalia V. Artemenko, Andrew G. McDonald, Gavin P. Davey, and Pauline M. Rudd -- Characterization of PEGylated biopharmaceutical products by LC/MS and LC/MS/MS / Lihua Huang and P. Clayton Gough -- Identification of asp isomerization in proteins by ¹⁸O labeling and tandem mass spectrometry / Jennifer Zhang and Viswanatham Katta -- Monitoring of subvisible particles in therapeutic proteins / Satish K. Singh and Maria R. Toler -- Size-exclusion chromatography with multi-angle light scattering for elucidating protein aggregation mechanisms / Erinc Sahin and Christopher J. Roberts -- Computational methods to predict therapeutic protein aggregation / Patrick M. Buck, Sandeep Kumar, Xiaoling Wang, Neeraj J. Agrawal, Bernhardt L. Trout, and Satish K. Singh -- Coarse-grained simulations of protein aggregation / Troy Cellmer and Nicolas L. Fawzi -- Chitosan-based nanoparticles as delivery systems of therapeutic proteins / Pedro Fonte, José Carlos Andrade, Vítor Seabra, and Bruno Sarmento -- Challenges in the development and manufacturing of antibody-drug conjugates / Laurent Ducry Summary After the identification of a potential protein drug, the next critical step is the production of sufficient authentic material for testing, characterization, and clinical trials, which, when successful, leads to the need for robust methodologies for large-scale production, purification, characterization, viral inactivation, and continued testing of the final protein product. Building on the valuable first edition, Therapeutic Proteins: Methods and Protocols, Second Edition aims to cover each of these key aspects of protein drug production through the contributions of authors from highly esteemed industrial and academic institutions around the world. Emphasizing the newest developments in the field, this second edition also includes additional emphasis on discovery, including new display and screening methods as well as the design and engineering of new types of therapeutic proteins. There is also discussion of computational and bioinformatics methods, and chapters on safety aspects of therapeutic protein development. Written in the highly successful Methods in Molecular Biology format, protocol chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and practical, Therapeutic Proteins: Methods and Protocols, Second Edition provides an essential resource to all scientists working in the field of therapeutic proteins Analysis farmacologie pharmacology toxicologie toxicology biomedische wetenschappen biomedicine eiwitexpressieanalyse proteomics Medicine (General) Geneeskunde (algemeen) Bibliography Includes bibliographical references and index Notes English Print version record Subject Proteins -- Therapeutic use Physical sciences. Genetic engineering. Pharmaceutical chemistry. Genetics -- Technique. Life sciences. Chemistry. Methodology. Protein engineering. Proteins. Pharmacology. Natural Science Disciplines Investigative Techniques Publication Formats Genetic Engineering Amino Acids, Peptides, and Proteins Chemistry, Pharmaceutical Analytical, Diagnostic and Therapeutic Techniques and Equipment Genetic Techniques Disciplines and Occupations Chemicals and Drugs Publication Characteristics Biological Science Disciplines Drug Discovery Chemistry Methods Laboratory Manuals Protein Engineering Proteins Pharmacology physical sciences. biological sciences. chemistry. methodology. protein. pharmacology. Proteins Protein engineering Physical sciences Pharmacology Pharmaceutical chemistry Methodology Life sciences Genetics -- Technique Genetic engineering Chemistry Proteins -- Therapeutic use Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Voynov, Vladimir. Caravella, Justin A., 1974- ISBN 9781617799211 1617799211

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