Title In vivo cellular imaging using fluorescent proteins : methods and protocols / edited by Robert M. Hoffman

Published New York : Humana Press, ©2012

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Description 1 online resource (xiii, 269 pages) : illustrations (some color) Series Methods in molecular biology, 1940-6029 ; 872 Methods in molecular biology (Clifton, N.J.) ; v. 872. 1064-3745 Contents Assessing Cancer Cell Migration and Metastatic Growth In Vivo in the Chick Embryo Using Fluorescence Intravital Imaging / Hon Sing Leong, Ann F. Chambers, and John D. Lewis -- The Use of Fluorescent Proteins for Intravital Imaging of Cancer Cell Invasion / James Hulit, Dmitriy Kedrin, Bojana Gligorijevic, David Entenberg, Jeffrey Wyckoff, John Condeelis, and Jeffrey E. Segall -- High-Resolution In Vivo Imaging of Fluorescent Proteins Using Window Chamber Models / Gregory M. Palmer, Andrew N. Fontanella, Siqing Shan, and Mark W. Dewhirst -- In Vivo Imaging of Pancreatic Cancer with Fluorescent Proteins in Mouse Models / Michael Bouvet and Robert M. Hoffman -- Lentivirus-Based DsRed-2-Transfected Pancreatic Cancer Cells for Deep In Vivo Imaging of Metastatic Disease / Zeqian Yu, Jiahua Zhou, and Robert M. Hoffman -- Noninvasive and Real-Time Fluorescence Imaging of Peritoneal Metastasis in Nude Mice / Hayao Nakanishi, Seiji Ito, Makoto Matsui, Yuichi Ito, Kazunari Misawa, and Yasuhiro Kodera -- Three-Dimensional In Vivo Imaging of Tumors Expressing Red Fluorescent Proteins / Alexander P. Savitsky, Irina G. Meerovich, Victoria V. Zherdeva, Lyaysan R. Arslanbaeva, Olga S. Burova, Darina V. Sokolova, Elena M. Treshchalina, Anatoly Yu Baryshnikov, Ilya I. Fiks, Anna G. Orlova, Michael S. Kleshnin, Ilya V. Turchin, and Alexander M. Sergeev -- Real-Time Visualization and Characterization of Tumor Angiogenesis and Vascular Response to Anticancer Therapies / H. Rosie Xing and Qingbei Zhang -- In Vivo Imaging of Human Cancer with Telomerase-Specific Replication-Selective Adenovirus / Toshiyoshi Fujiwara -- Real-Time Fluorescence Imaging of Abdominal, Pleural, and Lymphatic Metastases / Susanne Carpenter and Yuman Fong -- Real-Time Imaging of Tumors Using Replication-Competent Light-Emitting Microorganisms / Yong A. Yu, Stephanie Weibel, and Aladar A. Szalay -- GFP-Transgenic Animals for In Vivo Imaging: Rats, Rabbits, and Pigs / Takashi Murakami and Eiji Kobayashi -- The Use of Fluorescent Proteins for Developing Cancer-Specific Target Imaging Probes / Thomas E. McCann, Nobuyuki Kosaka, Peter L. Choyke, and Hisataka Kobayashi -- In Vivo Imaging of the Developing Mouse Embryonic Vasculature / Irina V. Larina and Mary E. Dickinson -- Screening Reef Corals for Novel GFP-Type Fluorescent Proteins by Confocal Imaging / Anya Salih -- What Does It Take to Improve Existing Fluorescent Proteins for In Vivo Imaging Applications? / Marc Zimmer -- In Vivo Imaging of Oligonucleotide Delivery / Fumitaka Takeshita, Ryou-u Takahashi, Jun Onodera, and Takahiro Ochiya -- Subcellular Imaging In Vivo: The Next GFP Revolution / Robert M. Hoffman Summary The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology. What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies Analysis Medicine Radiology, Medical Medicine & Public Health Imaging / Radiology geneeskunde volksgezondheid public health afbeelden imagery radiologie radiology Medicine (General) Geneeskunde (algemeen) Bibliography Includes bibliographical references and index Notes English Online resource; title from PDF title page (Springer Protocols, viewed Oct. 18, 2012) Subject Cancer -- Imaging -- Laboratory manuals Cancer cells -- Laboratory manuals Proteins. Diagnostic imaging. Diseases. Methodology. Diagnosis. Publication Formats Investigative Techniques Proteins Diagnostic Imaging Analytical, Diagnostic and Therapeutic Techniques and Equipment Disease Image Processing, Computer-Assisted Diagnostic Techniques and Procedures Computing Methodologies Publication Characteristics Amino Acids, Peptides, and Proteins Information Science Chemicals and Drugs Neoplasms Methods Diagnosis Imaging, Three-Dimensional Luminescent Proteins Laboratory Manuals protein. methodology. diagnosis. Proteins Methodology Diseases Diagnostic imaging Diagnosis Cancer cells Cancer -- Imaging Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Hoffman, Robert M., 1944- ISBN 9781617797972 1617797979 1617797960 9781617797965

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