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E-book
Author Nicholl, Desmond S. T.

Title An introduction to genetic engineering / Desmond S.T. Nicholl
Edition Second edition
Published New York : Cambridge University Press, 2002
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Description 1 online resource (xii, 292 pages) : illustrations
Series Studies in biology
Studies in biology.
Contents The basis of genetic engineering -- Introducing molecular biology -- The flow of genetic information -- The structure of DNA and RNA -- Gene organisation -- Gene structure in prokaryotes -- Gene structure in eukaryotes -- Gene expression -- Genes and genomes -- Genome size and complexity -- Genome organisation -- Working with nucleic acids -- Isolation of DNA and RNA -- Handling and quantification of nucleic acids -- Radiolabelling of nucleic acids -- End labelling -- Nick translation -- Labelling by primer extension -- Nucleic acid hybridisation -- Gel electrophoresis -- DNA sequencing -- Maxam--Gilbert (chemical) sequencing -- Sanger--Coulson (dideoxy or enzymatic) sequencing -- Electrophoresis and reading of sequences -- The tools of the trade -- Restriction enzymes--cutting DNA -- Type II restriction endonucleases -- Use of restriction endonucleases -- Restriction mapping -- DNA modifying enzymes -- Nucleases -- Polymerases -- Enzymes that modify the ends of DNA molecules -- DNA ligase--joining DNA molecules -- The methodology of gene manipulation -- Host cells and vectors -- Host cell types -- Prokaryotic hosts -- Eukaryotic hosts -- Plasmid vectors for use in E. coli -- What are plasmids? -- Basic cloning plasmids -- Slightly more exotic plasmid vectors -- Bacteriophage vectors for use in E. coli -- What are bacteriophages? -- Vectors based on bacteriophage [lambda] -- Vectors based on bacteriophage M13 -- Other vectors -- Hybrid plasmid/phage rectors -- Vectors for use in eukaryotic cells -- Artificial chromosomes
Summary Des Nicholl presents here a new, fully revised, and expanded edition of his popular undergraduate-level textbook. Many of the features of the original edition have been retained; the book still offers a concise technical introduction to the subject of genetic engineering. However, the book is now divided into three main sections: the first introduces students to basic molecular biology, the second section explains the methods used to manipulate genes, and the third deals with modern applications of genetic engineering. A whole chapter is now devoted to the polymerase chain reaction. Applications covered in the book include genomics, protein engineering, gene therapy, cloning, and transgenic animals and plants. A final chapter discusses the ethical questions surrounding genetic engineering in general. An Introduction to Genetic Engineering is essential reading for undergraduate students of biotechnology, genetics, molecular biology and biochemistry
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Genetic engineering.
Genetic Engineering.
Form Electronic book
ISBN 0511252587 (electronic bk. ; Adobe Reader)
0511336896
0511648677 (electronic bk.)
1139168207 (electronic bk.)
9780511252587 (electronic bk. ; Adobe Reader)
9780511336898
9780511648670 (electronic bk.)
9781139168205 (electronic bk.)
(paperback)
(hardback)