| Description |
1 online resource (xxxvii, 413 pages, 16 unnumbered pages of plates) : illustrations (some color) |
| Series |
Methods in enzymology, 0076-6879 ; v. 388 |
|
Methods in enzymology ; v. 388. 0076-6879
|
| Contents |
Section I. Methodology -- Gene site saturation mutagenesis : a comprehensive mutagenesis approach -- Single-stranded DNA family shuffling -- Module shuffling -- SCHEMA-guided protein recombination -- Staggered extension process in vitro DNA recombination -- Using incremental truncation to create libraries of hybrid enzymes -- Random multirecombinant polymerase chain reaction -- Gene library synthesis by structure-based combinatorial protein engineering -- New enzymes from combinatorial library modules -- Construction of protein fragment complementation libraries using incremental truncation -- Section II. Applications: optimization and screening -- GigaMatrix: a novel ultrahigh throughput protein optimization and discovery platform -- High throughput microplate screens for directed protein evolution -- Periplasmic expression as a basis for whole cell kinetic screening of unnatural enzyme reactivities -- Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis -- Screening for oxidative resistance -- Consensus-based engineering of protein stability: from intrabodies to thermostable enzymes -- Improving the functional expression of N-Carbamoylase by directed evolution using the green fluorescent protein fusion reporter system -- Section III. Applications: directed evolution of enzymatic function -- Directed evolution of lipases and esterases -- Protein engineering of the cytochrome P450 monooxygenase from bacillus megaterium -- Directed evolution of aldolases -- Changing the enantioselectivity of enzymes by directed evolution -- Control of stereoselectivity in phosphotriesterase -- Section IV. Applications: evolution of biosynthetic pathways -- Manipulation and analysis of polyketide synthases -- Reactions catalyzed by mature and recombinant nonribosomal peptide synthetases -- Engineering carotenoid biosynthetic pathways -- Section V. Devices, antibodies, and vaccines -- Directed evolution of bacteriorhodopsin for device applications -- Engineering antibody affinity by yeast surface display -- A conditionally replicating virus as a novel approach toward an HIV vaccine |
| Summary |
This MIE volume covers methods for a multitude of topics among which are computational methods, laboratory methods, enzyme optimization, binding proteins/antibodies, and screening technologies |
| Bibliography |
Includes bibliographical references and indexes |
| Notes |
Print version record |
| Subject |
Protein engineering.
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Proteins -- Synthesis.
|
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Genetic engineering.
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Proteins -- Structure-activity relationships.
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Genetic Engineering.
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Protein Conformation.
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Proteins -- genetics.
|
| Form |
Electronic book
|
| Author |
Robertson, Dan E.
|
|
Noel, Joseph P.
|
| ISBN |
9780080497259 (electronic bk.) |
|
008049725X (electronic bk.) |
|
0121827933 (electronic bk.) |
|
9780121827939 (electronic bk.) |
|
1281011541 |
|
9781281011541 |
|
