| Description |
1 online resource (li, 337 pages, 16 unnumbered pages of plates) : illustrations (some color) |
| Series |
Methods in enzymology ; vol. 512 |
|
Methods in enzymology ; v. 512
|
| Contents |
Examining histone posttranslational modification patterns by high-resolution mass spectrometry -- Investigating the cell cycle-associated dynamics of histone modifications using quantitative mass spectrometry -- A method to site-specifically incorporate methyl-lysine analogues into recombinant proteins -- Methods applied to the study of protein arginine methylation -- Enzymatic analysis of Tet proteins: key enzymes in the metabolism of DNA methylation -- Peptide mciroarrays to interrogate the "histone code" -- Identifying chromatin readers using a SILAC-based histone peptide pull-down approach -- High-throughput strategy to identify inhibitors of histone-binding domains -- Validation of histone-binding partners by peptide pull-downs and isothermal titration calorimetry -- Analysis of histone chaperone antisilencing function 1 interactions -- Quantifying chromatin-associated interactions: the HI-FI system -- Biochemical assay for histone H2A.Z replacement by the yeast SWR1 chromatin remodeling complex -- Experimental analysis of the mechanism of chromatin remodeling by RNA polymerase II |
| Summary |
This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 2 volumes covering Nucleosomes, Histones & Chromatin and has chapters on Methods applied to the study of Protein Arginine Methylation, High resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and Peptide arrays to interrogate the binding specificity of chromatin-binding proteins. Continues the legacy of this premier serial by containing quality chapters authored by leaders in the field The first of 2 volumes covering nucleosomes, histones and chromatin Chapters on methods applied to the study of protein arginine methylation, high-resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins |
| Bibliography |
Includes bibliographical references and indexes |
| Notes |
Description based on print version record |
| Subject |
Chromatin.
|
|
Histones.
|
|
Nucleoproteins.
|
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Chromatin.
|
|
Histones.
|
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Nucleoproteins.
|
|
Nucleosomes.
|
| Form |
Electronic book
|
| Author |
Allis, C. David.
|
|
Wu, Carl.
|
| ISBN |
0123919401 |
|
012391941X (electronic bk.) |
|
9780123919403 |
|
9780123919410 (electronic bk.) |
|
