Limit search to available items
Book Cover
E-book

Title The nucleic acid protocols handbook / edited by Ralph Rapley
Published Totowa, N.J. : Humana Press, ©2000

Copies

Description 1 online resource (xxii, 1050 pages) : illustrations
Series Methods in Molecular Biology
Methods in molecular biology.
Contents pt. 1. Nucleic acid extraction -- pt. 2. Basic separation and analysis of DNA -- pt. 3. Probe design, synthesis, and labeling -- pt. 4. RNA analyis techniques -- pt. 5. Gene library construction and screening -- pt. 6. DNA screening -- pt. 7. Basic polymerase chain reaction methods -- pt. 8. Analyzing genes, mutations, and protein interactions -- pt. 9. Mutagenesis, transcription, and translation in vitro pt. 10. Gene localization, mapping in situ, and bioinformatics
Summary The Nucleic Acid Protocols Handbook is today's most comprehensive up-to-date treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular Biology series, providing an introduction to the scientific basis of each technique, a complete listing of all the materials and reagents needed, and clear step-by-step instructions to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success in the lab. The authord are well-experienced masters of the techniques described, often having perfected and optimized them in their own laboratories. In The Nucleic Acid Protocols Handbook, Ralph Rapley has brought together the widest-ranging, one-volume collection of classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids. Eminently practical and reproducible, these proven methods will help both experienced researchers and those entering the field attain the laboratory mastery needed-not only to deepen understanding of biological processes at the molecular level, but also to begin realizing the biotechnological bounty so clearly in prospect
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Nucleic acids -- Laboratory manuals
Polymerase chain reaction -- Laboratory manuals
Gene mapping -- Laboratory manuals
Molecular genetics -- Laboratory manuals
Nucleic Acids -- isolation & purification
Chromosome Mapping -- methods
Molecular Biology -- methods
Polymerase Chain Reaction -- methods
SCIENCE -- Life Sciences -- Molecular Biology.
Gene mapping
Molecular genetics
Nucleic acids
Polymerase chain reaction
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Rapley, Ralph.
ISBN 9781592590384
1592590381
0585399387
9780585399386
0896034593
9780896034594
1280820330
9781280820335
9786610820337
6610820333