| Description |
1 online resource (xi, 372 pages) : illustrations (chiefly color) |
| Series |
Methods in Molecular Biology, 1064-3745 ; 1538 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1538. 1064-3745
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| Contents |
Cell culture system to investigate the presynaptic control of subsynaptic membrane differentiation at the neuromuscular junction / Nadine Schmidt, Sreya Basu, Stephan Kröger, and Hans Rudolf Brenner -- Co-culture synaptogenic assay : a new look at fluorescence reporters and technological devices / Karen Perez de Arce and Massimiliano Stagi -- Synaptogenic assays using neurons cultured on micropatterned substrates / Katalin Czöndör and Olivier Thoumine -- Monitoring synapses via trans-synaptic GFP complementation / Theodoros Tsetsenis -- Generation of spinal motor neurons from human pluripotent stem cells / David P. Santos and Evangelos Kiskinis -- Biochemical purification of binding partners of synaptic scaffold proteins / Bekir Altas, Olaf Jahn, and Hiroshi Kawabe -- In situ protein binding assay using Fc-fusion proteins / Nirmala Padmanabhan and Tabrez J. Siddiqui -- Reconstitution of synaptic SNAREs into large liposomes with reduced curvature stress / Javier M. Hernandez -- Isolation of synaptosomes, synaptic plasma membranes, and synaptic junctional complexes / Mary L. Michaelis, Lei Jiang, and Elias K. Michaelis -- Purification of synaptosome populations using fluorescence-activated synaptosome sorting / Elisa Luquet, Christoph Biesemann, Annie Munier, and Etienne Herzog -- Optimized protocol for imaging cleared neural tissues using light microscopy / Yoh Isogai, Douglas S. Richardson, Catherine Dulac, and Joseph Bergan -- Structured illumination microscopy for the investigation of synaptic structure and function / Soyon Hong, Daniel K. Wilton, Beth Stevens, and Douglas S. Richardson -- 3D d STORM imaging of fixed brain tissue / Frank Herrmannsdörfer, Benjamin Flottmann, Siddarth Nanguneri, Varun Venkataramani, Heinz Horstmann, Thomas Kuner, and Mike Heilemann -- Photomarking relocalization technique for correlated two-photon and electron microcopy imaging of single stimulated synapses / Miquel Bosch, Jorge Castro, Mriganka Sur, and Yasunori Hayashi -- 3D analysis of synaptic ultrastructure in organotypic hippocampal slice culture by high-pressure freezing and electron tomography / Cordelia Imig and Benjamin H. Cooper -- Analyzing endosomal docking, fusion, sorting, and budding mechanisms in isolated organelles / Sina V. Barysch and Ioanna Bethani -- Concurrent imaging of receptor trafficking and calcium dynamics by spinning disk confocal microscopy / DeLaine D. Larsen, Regina Wai-Yan Choy, and Minjong Park -- Imaging activity-dependent signaling dynamics at the neuronal synapse using FRET-based biosensors / Zohreh Farsi and Andrew Woehler -- Analyzing structural plasticity of dendritic spines in organotypic slice culture / Mathias De Roo and Adema Ribic -- Using fluorescent markers to estimate synaptic connectivity in situ / Mrinalini Hoon, Raunak Sinha, and Haruhisa Okawa -- Dual anterograde and retrograde viral tracing of reciprocal connectivity / Matthias G. Haberl, Melanie Ginger, and Andreas Frick -- Mapping synaptic inputs of developing neurons using calcium imaging / Johan Winnubst and Christian Lohmann -- Monosynaptic tracing in developing circuits using modified rabies virus / Laura Cocas and Gloria Fernandez |
| Summary |
This detailed volume collects protocols for experimentation into how neurons connect to produce the extraordinary functionalities of the nervous system. Contributed by experts and pioneers in their respective techniques, the book covers synapses in the brain and in culture, their constituents, their structures, their dynamics, and the assemblies they form, all in the structure of a laboratory guide. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synapse Development: Methods and Protocols serves as an ideal guide to minimizing the barrier to entry for the integration of new approaches with existing expertise, producing syntheses that will foster novel perspectives on the many ways in which synapses form, transform, and transmit |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed December 12, 2016) |
| Subject |
Synapses -- Laboratory manuals
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Synapses.
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Synapses
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Laboratory Manuals
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Synapses
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Poulopoulos, Alexandros, editor
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| ISBN |
9781493966882 |
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149396688X |
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