Title Biosystems engineering I : creating superior biocatalysts / volume editors, Christoph Wittmann, Rainer Krull ; with contributions by R. Biedendieck [and others]

Published Berlin ; New York : Springer, ©2010

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Description 1 online resource (xiii, 247 pages) : illustrations Series Advances in biochemical engineering/biotechnology, 1616-8542 ; 120 Advances in biochemical engineering/biotechnology ; 120. 0724-6145 Contents Note continued: 4.1. Characteristics and Applications of S. cerevisiae as Cell Factory -- 4.2. Strain Improvements of Baker and Brewer Yeast -- 4.3. S. cerevisiae as a Platform for Chemical Production -- 5. Fungal Cell Factories -- 5.1. Characteristics and Applications of Aspergillus Species as Cell Factories -- 5.2. Strain Improvement of the Industrial Production Hosts A. oryzae and A. niger -- 5.3. Aspergillus Species as Platform for Production of Chemicals -- 5.4. Heterologous Protein Production -- 6. Systems Biology as Driver for Industrial Biotechnology -- 6.1. Yeast Examples -- 6.2. Aspergillus Examples -- References -- De Novo Metabolic Engineering and the Promise of Synthetic DNA / Gregory N. Stephanopoulos -- 1. Introduction -- 2. Brief History of Oligonucleotide Synthesis -- 3. Molecular Engineering for the Control of Gene Expression -- 3.1. Re-Engineering Translational Control -- 3.2. Modulating the Transcriptional Machinery of the Cell -- 4. Exploring New Possibilities with Hitherto Elusive Genes -- 5. Gene Circuit Engineering Using Synthetic DNA Technology -- 6. Synthetic Libraries: Beyond Conventional Pathway Engineering -- 7. Next Frontier in Metabolic Engineering: The Road to Synthetic Organisms -- 8. Conclusions -- References -- Systems Biology of Recombinant Protein Production in Bacillus megaterium / Dieter Jahn -- 1. Introduction: What is Bacillus megaterium? -- 2. Tools for Recombinant Protein Production with Bacillus megaterium -- 2.1. Xylose-Inducible Promoter System -- 2.2. Expression Vectors for the Intracellular Production of Affinity Tagged Recombinant Proteins -- 2.3. Intracellular Recombinant Production and Affinity Chromatographic Purification of Proteins -- 2.4. High Cell Density Cultivations of GFP Producing Bacillus megaterium -- 2.5. Expression Vectors for the Export of Recombinant Proteins into the Growth Medium Note continued: 2.6. Production, Export and Affinity Chromatographic Purification of Recombinant Proteins from the Growth Medium -- 2.7. Development of Multiple Vector Systems -- 2.8. T7 RNA Polymerase Dependent Promoter System -- 2.9. Culture Heterogeneity During Intracellular Protein Production -- 3. Towards Systems Biology -- 3.1. Genome Sequences of Two Bacillus megaterium Strains -- 3.2. Metabolic Flux Analysis in Bacillus megaterium -- 4. Systems Biology with Bacillus megaterium? -- References -- Extending Synthetic Routes for Oligosaccharides by Enzyme, Substrate and Reaction Engineering / Klaus Buchholz -- 1. Introduction -- 2. Enzymatic Oligosaccharide Synthesis -- 2.1. Acceptor Reactions of Dextransucrase, Products, Kinetics -- 2.2. Engineering of Glycosyltransferases (GTF, FTF) -- 3. From Gene to Product -- 3.1. Biocatalyst Development (Immobilization, Two Enzyme System) -- 3.2. Engineering of GTFR -- 3.3. Application of Engineered Dextransucrase (GTFR) -- 3.4. Integrated Reaction Engineering -- 4. Conclusions -- References -- Regeneration of Nicotinamide Coenzymes: Principles and Applications for the Synthesis of Chiral Compounds / Werner Hummel -- 1. Introduction -- 2. Enzymatic Methods to Regenerate Reduced Nicotinamide Cofactors -- 2.1. Formate Dehydrogenase -- 2.2. Glucose-6-Phosphate Dehydrogenase -- 2.3. Glucose Dehydrogenase -- 2.4. Alcohol Dehydrogenase -- 2.5. Hydorgenase -- 2.6. Phosphite Dehydrogenase -- 2.7. Further Enzyme Systems for the Regeneration of Reduced Nicotinamide Cofactors -- 3. Enzymatic Methods to Regenerate Oxidized Nicotinamide Cofactors -- 3.1. Glutamate Dehydrogenase -- 3.2. L-Lactate Dehydrogenase -- 3.3. Alcohol Dehydrogenase -- 3.4. NADH Oxidase -- 4. Electrochemical Methods -- 4.1. Electroenzymatic Oxidations -- 4.2. Electroenzymatic Reductions -- 5. Further Methods Note continued: 5.1. Photoelectrochemical Cofactor Regeneration -- 5.2. Chemical Cofactor Regeneration -- 6. Whole Cell Applications Based on Recombinant Enzymes ("Designer Cells") -- 7. Coenzyme-Dependent Reactions in Organic Solvents -- 8. Nicotinamide Dinucleotide Binding Motif and Its Reversal by Protein Engineering -- 9. Examples of Small-Scale and Industrial Applications -- 9.1. L-tert-Leucine as an Example of Ton-Scale Synthesis -- 9.2. L-6-Hydroxynorleucine -- 9.3. Synthesis of Allysine Ethylene Acetal in 80 kg-Scale -- 9.4. Synthesis of Chiral Alcohols -- 9.5. Alcohol Dehydrogenase Catalyzed Synthesis of the Chiral Side Chain of Statins -- 9.6. Synthesis of Optically Active L-Amino Acids with Amino Acid Dehydrogenases -- 9.7. Optically Active & alpha;-Hydroxy Acids or & alpha;-Hydroxy Esters -- 9.8. Multistep "One-Pot" Processes: Direct Coupling of a Chemical and an Enzymatic Step -- 10. Conclusions: Capability and Limitations -- References Summary -Integration of Systems Biology with Bioprocess Engineering: L-Threonine Production by Systems Metabolic Engineering of Escherichia Coli, By Sang Yup Lee and Jin Hwan Park; -Analysis and Engineering of Metabolic Pathway Fluxes in Corynebacterium glutamicum, By Christoph Wittmann; -Systems Biology of Industrial Microorganisms, Marta Papini, Margarita Salazar, and Jens Nielsen; -De Novo Metabolic Engineering and the Promise of Synthetic DNA, By Daniel Klein-Marcuschamer, Vikramaditya G. Yadav, Adel Ghaderi, and Gregory N. Stephanopoulos; -Systems Biology of Recombinant Protein Production in Bacillus megaterium, Rebekka Biedendieck, Boyke Bunk, Tobias Fürich, Ezequiel Franco-Lara, Martina Jahn, and Dieter Jahn; -Extending Synthetic Routes for Oligosaccharides by Enzyme, Substrate and Reaction Engineering; By Jürgen Seibel, Hans-Joachim Jördening, and Klaus Buchholz; -Regeneration of Nicotinamide Coenzymes: Principles and Applications for the Synthesis of Chiral Compounds; By Andrea Weckbecker, Harald Gröger, and Werner Hummel Bibliography Includes bibliographical references and index Notes English In Springer eBooks Subject Bioengineering. Enzymes -- Biotechnology Systems biology. Biotechnology Systems Biology bioengineering. Chimie. Science des matériaux. Bioengineering Enzymes -- Biotechnology Systems biology Genre/Form Festschriften Festschriften. Form Electronic book Author Wittmann, Christoph Krull, Rainer Biedendieck, R. (Rebekka) ISBN 9783642142314 3642142311 1283510936 9781283510936 9786613510938 6613510939

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