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Book Cover
Book
Author Li, S. F. Y. (Sam Fong Yau), 1957-

Title Capillary electrophoresis : principles, practice, and applications / S.F.Y. Li
Published Amsterdam ; New York : Elsevier, 1992

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Location Call no. Vol. Availability
 W'PONDS  541.372 Lia/Cep  TEMPORARILY UNAVAILABLE
Description xxvi, 582 pages : illustrations ; 25 cm
Series Journal of chromatography library ; v. 52
Journal of chromatography library ; v. 52
Contents Preface -- List of Symbols -- Abbreviations -- Ch. 1. Introduction -- 1.1. Historical background -- 1.2. Different modes of capillary electrophoresis -- 1.3. Principles of separation in capillary zone electrophoresis (CZE) -- 1.4. Comparison with other separation techniques -- Ch. 2. Sample Injection Methods -- 2.2. Electrokinetic injection -- 2.3. Hydrodynamic injection -- 2.4. Electric sample splitter -- 2.5. Split flow syringe injection system -- 2.6. Rotary-type injector -- 2.7. Freeze plug injection -- 2.8. Sampling device with feeder -- 2.9. Microinjectors -- 2.10. Optical gating -- 2.11. On-column fracture for sample introduction -- Ch. 3. Detection Techniques -- 3.2. UV-visible absorbance detectors -- 3.3. Photodiode array and multiwavelength UV detection -- 3.4. Fluorescence detection -- 3.5. Laser-based thermo-optical and refractive index detectors -- 3.6. Electrochemical detection -- 3.7. Indirect detection -- 3.8. Radioisotope detectors -- 3.9. Mass spectrometric detection -- Ch. 4. Column Technology -- 4.2. Coated columns -- 4.3. Columns for capillary gel electrophoresis (CGE) -- 4.4. Packed columns -- 4.5. Capillary electrophoresis on a chip -- Ch. 5. Electrolyte Systems -- 5.2. Micellar electrokinetic chromatography (MEKC) -- 5.3. Use of inclusion complexes -- 5.4. Complexing additives -- 5.5. Other types of electrophoretic media -- Ch. 6. Special Systems and Methods -- 6.2. Buffer programming -- 6.3. Fraction collection -- 6.4. Field effect electroosmosis -- 6.5. Systematic optimization schemes -- 6.6. Determination of electrophoretic mobilities and diffusion coefficients -- 6.7. Capillary isoelectric focusing (CIEF) -- 6.8. Capillary isotachophoresis -- 6.9. Hyphenated techniques -- Ch. 7. Applications -- 7.1. Amino acids -- 7.2. Peptides -- 7.3. Proteins -- 7.4. Nucleic acids -- 7.5. Pharmaceuticals and drugs -- 7.6. Cells, viruses and bacteria -- 7.7. Analysis of body fluids -- 7.8. Capillary ion analysis -- 7.9. Metal chelates -- 7.10. Organic compounds -- 7.11. Carbohydrates -- 7.12. Food analysis -- 7.13. Environmental analysis -- 7.14. Polymer and particle analysis -- 7.15. Natural products -- 7.16. Chiral separation -- 7.17. Separation of geometrical and positional isomers -- 7.18. Coal and fuels -- 7.19. Textile and dyes -- 7.20. Explosives -- 7.21. Survey of commercial CE instruments -- 7.22. Summary of applications of CE -- Ch. 8. Recent Advances and Prospect for Growth -- 8.1. Recent reviews on CE -- 8.2. Advances in injection techniques -- 8.3. Novel detection techniques -- 8.4. Advances in column technology -- 8.5. Progress on electrolyte systems -- 8.6. New systems and methods -- 8.7. Additional applications based on CE -- 8.8. Future trends -- Subject index
Summary Capillary Electrophoresis (CE) has had a very significant impact on the field of analytical chemistry in recent years as the technique is capable of very high resolution separations, requiring only small amounts of samples and reagents. Furthermore, it can be readily adapted to automatic sample handling and real time data processing. Many new methodologies based on CE have been reported. Rapid, reproducible separations of extremely small amounts of chemicals and biochemicals, including peptides, proteins, nucleotides, DNA, enantiomers, carbohydrates, vitamins, inorganic ions, pharmaceuticals and environmental pollutants have been demonstrated. A wide range of applications have been developed in greatly diverse fields, such as chemical, biotechnological, environmental and pharmaceutical analysis. This book covers all aspects of CE, from the principles and technical aspects to the most important applications. It is intended to meet the growing need for a thorough and balanced treatment of CE. The book will serve as a comprehensive reference work. Both the experienced analyst and the newcomer will find the text useful
Analysis Electrochemistry
Bibliography Includes bibliographical references and index
Subject Capillary electrophoresis.
Electrophoresis.
LC no. 92014151
ISBN 0444815902
0444894330