Protein purification laboratory -- Making an extract -- Analysis: measurement of protein and enzyme activity -- Separation by precipitation -- Separation by adsorption I: general aspects -- Separation by adsorption II: ion exchangers and nonspecific adsorbents -- Separation by adsorption: affinity techniques -- Separation in solution -- Purification of special types of proteins -- Small-scale and large-scale procedures -- Analysis for purity -- Optimization of procedures: final steps -- Precipitation tables -- Solutions for measuring protein concentration -- Buffers for use in protein chemistry -- Chromatographic materials
Summary
A guide providing a brief account of the main protein fractionation methods, with some simple theoretical and thermodynamic explanations of the events occurring, for students and researchers involved in the process of isolating an enzyme, from whatever source. A basic background in biochemistry and protein chemistry is assumed
Analysis
Proteins Chemical analysis
Proteins Chemical analysis
Notes
Includes index
Bibliography
Includes bibliographical references (pages 356-373) and index
