| Description |
1 online resource (xlv, 354 pages, 8 unnumbered pages of plates) : illustrations (some color) |
| Series |
Methods in enzymology, 0076-6879 ; v. 488 |
|
Methods in enzymology ; v. 488
|
| Contents |
1. Measurement and analysis of equilibrium binding titrations : a beginner's guide / Dorothy Beckett -- 2. Macromolecular competition titration method : accessing thermodynamics of the unmodified macromolecule-ligand interactions through spectroscopic titrations of fluorescent analogs / Wlodzimierz Bujalowksi and Maria J. Jezewska -- 3. Analysis of PKR-RNA interactions by sedimentation velocity / C. Jason Wong, Katherine Launer-Felty, and James L. Cole -- 4. Structural and thermodynamic analysis of PDZ-ligand interactions / Tyson R. Shepherd and Ernesto J. Fuentes -- 5. Thermodynamic analysis of metal ion-induced protein assembly / Andrew B. Herr and Deborah G. Conrady -- 6. Thermodynamic dissection of colicin interactions / Nicholas G. Housden and Colin Kleanthous -- 7. Energetics of Src homology domain interactions in receptor tyrosine kinase-mediated signaling / John E. Ladbury and Stefan T. Arold -- 8. Structural and functional energetic linkages in allosteric regulation of muscle pyruvate kinase / J. Ching Lee and Petr Herman -- 9. Analysis of free energy versus temperature curves in protein folding and macromolecular interactions / Vince J. LiCata and Chin-Chi Liu -- 10. Application of the sequential n-step kinetic mechanism to polypeptide translocases / Aaron L. Lucius, Justin M. Miller, and Burki Rajendar -- 11. A coupled equilibrium approach to study nucleosome thermodynamics / Andrew J. Andrews and Karolin Luger -- 12. Quantitative methods for measuring DNA flexibility in vitro and in vivo / Justin P. Peters, Nicole A. Becker, Emily M. Rueter, Zeljko Bajzer, Jason D. Kahn, and L. James Maher III |
| Summary |
The use of thermodynamics in biological research can be equated to an energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. Elucidates the relationships between structure and energetics and their applications to molecular design, aiding researchers in the design of medically important molecules |
| Bibliography |
Includes bibliographical references and indexes |
| Notes |
Description based on print version record |
| Subject |
Ligands (Biochemistry)
|
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Protein binding.
|
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Protein-protein interactions.
|
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Thermodynamics.
|
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Hemoglobins.
|
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Ligands.
|
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Protein Binding.
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Thermodynamics.
|
| Form |
Electronic book
|
| Author |
Ackers, Gary K.
|
|
Holt, Jo M.
|
|
Johnson, Michael L., 1947-
|
| ISBN |
0123812682 (electronic bk.) |
|
0123812690 |
|
9780123812681 (electronic bk.) |
|
9780123812698 |
|
