Title Single cell diagnostics : methods and protocols / edited by Alan Thornhill

Published Totowa, N.J. : Humana Press, ©2007

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Description 1 online resource (xi, 182 pages) : illustrations (some color) Series Methods in molecular medicine ; 132 Methods in molecular medicine ; 132. 1543-1894 Contents Noninvasive metabolic assessment of single cells / David K. Gardner -- Laser capture microdissection for analysis of single cells / Nobuki Nakamura [and others] -- Fluorescence in situ hybridization (FISH) on single cells (sex determination and chromosome rearrangements) / Paul N. Scriven and Caroline Mackie Ogilvie -- Single cell PCR for PGD : methods, strategies, and limitations / Karen Sermon and Martine De Rycke -- Real-time quantitative PCR measurement of male fetal DNA in maternal plasma / Bernhard Zimmermann [and others] -- Cell-free fetal DNA plasma extraction and real-time PCR quantification / Jill L. Maron, Kirby L. Johnson, and Diana W. Bianchi -- LATE-PCR and allied technologies : real-time detection strategies for rapid, reliable diagnosis from single cells / Kenneth E. Pierce and Lawrence J. Wangh -- Efficient isothermal amplification of the entire genome from single cells / Karen V. Schowalter, Jolene R. Fredrickson, and Alan R Thornhill -- Comparative genomic hybridization on single cells / Lucille Voullaire and Leeanda Wilton -- Generation of amplified RNAs and cDNA libraries from single mammalian cells / James Adjaye -- Real-time PCR to measure gene expression in single cells / Dagan Wells -- Gender determination and detection of aneuploidy in single cells using DNA array-based comparative genomic hybridization (array CGH) / Dong Gui Hui, Xin Yuan Guan, and Nicole Hussey -- Microarray technology for mutation analysis of low template DNA samples / Chelsea Salvado and David Cram Summary The aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR) As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells Bibliography Includes bibliographical references and index Notes English Print version record Subject Cytogenetics. Molecular diagnosis. Genetic screening. Infertility. Polymerase chain reaction. Cytogenetic Analysis Molecular Diagnostic Techniques Preimplantation Diagnosis -- methods Infertility -- therapy Polymerase Chain Reaction Cytogenetics Genetic Testing Infertility SCIENCE -- Life Sciences -- Molecular Biology. Molecular diagnosis. Genetic Screening. Infertility. Cytogenetic Analysis. Molecular Diagnostic Techniques. Infertility -- therapy. Polymerase chain reaction. Preimplantation Diagnosis -- methods. Cytogenetics. Polymerase chain reaction Cytogenetics Genetic screening Infertility Molecular diagnosis Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Thornhill, Alan. ISBN 1588295788 9781588295781 9781597452984 159745298X 1280972106 9781280972102 9786610972104 6610972109

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