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Title Cell-based assays for high-throughput screening : methods and protocols / edited by Paul A. Clemons, Nicola J. Tolliday, and Bridget K. Wagner
Published New York : Humana, ©2009


Description 1 online resource (x, 218 pages) : illustrations
Series Methods in molecular biology, 1064-3745 ; v. 486
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 486
Springer protocols (Series)
Contents Introduction: Cell-based assays for high-throughput screening / W.F. An and N.J. Tolliday -- High-throughput screening of model bacteria / S. Zlitni, J.E. Blanchard and E.D. Brown -- Cell-based assays to probe the ERK MAP kinase pathway in endothelial cells / M.R. Wyler [and others] -- Large-scale small-molecule screen using zebrafish embryos / C.C. Hong -- Whole-animal high-throughput screens: The C. elegans model / E.J. O'Rourke, A.L. Conery and T.I. Moy -- Whole-organism screening: Plants / A. Agee and D. Carter -- Fluorescence-based assays / W.F. An -- Reporter gene assays / A.M.F. Liu [and others] -- Screening for chemical inhibitors of heterologous proteins expressed in yeast using a simple growth-restoration assay / A.D. Balgi and M. Roberge -- Assay for isolation of inhibitors of her2-kinase expression / G. Chiosis and A.B. Keeton -- High-content screening: Flow cytometry analysis / B.S. Edwards [and others] -- High-throughput real-time PCR for detection of gene-expression levels / B.K. Wagner and Z. Arany -- Interpretation of uniform-well readouts / S. Josiah -- Extracting rich information from images / A.E. Carpenter
Summary As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches
Bibliography Includes bibliographical references and index
Notes English
Subject High throughput screening (Drug development)
Biological assay.
Drug Discovery
Biological Assay
Drug Evaluation, Preclinical
Biological assay
High throughput screening (Drug development)
Dosage biologique -- Cahiers de laboratoire.
Évaluation préclinique médicament -- Cahiers de laboratoire.
High throughput screening
Biology - General.
Pharmacy, Therapeutics, & Pharmacology.
Health & Biological Sciences.
Physical Sciences & Mathematics.
Pharmacie -- Recherche.
Essais biologiques.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Clemons, Paul A.
Tolliday, Nicola J.
Wagner, Bridget K.
ISBN 9781603275446