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Title Immune checkpoint blockade : methods and protocols / edited by Yago Pico de Coaña
Published New York : Humana Press : Springer, [2019]


Description 1 online resource (xi, 225 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume 1913
Methods in molecular biology (Clifton, N.J.) ; v. 1913. 1064-3745
Contents Double immunohistochemistry and digital image analysis / Pablo Moreno-Ruiz, Lina Wik Leiss, Artur Mezheyeuski, and Monika Ehnman -- Multispectral fluorescence imaging allows for distinctive topographic assessment and subclassification of tumor-infiltrating and surrounding immune cells / Claudia Wickenhauser, Daniel Bethmann, Zipei Feng, Shawn M. Jensen, Carmen Ballesteros-Merino, Chiara Massa, Andre Steven, Marcus Bauer, Peter Kaatzsch, Nikolaos Pazaitis, Georgiana Toma, Carlo B. Bifulco, Bernard A. Fox, and Barbara Seliger -- Systems-level immune monitoring by mass cytometry / Tadepally Lakshmikanth and Petter Brodin -- Immune monitoring of cancer patients by multi-color flow cytometry / Shi Yong Neo, Aine O'Reilly, and Yago Pico de Coana -- High-throughput, fast, and sensitive immunopeptidomics sample processing for mass spectrometry / Fabio Marino, Chloe Chong, Justine Michaux, and Michal Bassani-Sternberg -- Identification of immune modulatory miRNAs by miRNA enrichment via RNA affinity purification / Uta Sandy Tretbar, Michael Friedrich, Maria-Filothei Lazaridou, and Barbara Seliger -- Expansion of tumor-infiltrating lymphocytes from melanoma tumors / Stina Wickstrom and Tanja Lovgren -- Establishment of two dimensional (2D) and three-dimensional (3D) melanoma primary cultures as a tool for in vitro drug resistance studies / Nataly Cruz Rodriguez, Johanna Lineros, Carol Stefany Rodrłguez, Lina Maria Martinez, and Josefa Antonia Rodriguez -- Assessment of antitumor T-cell responses by flow cytometry after coculture of tumor cells with autologous tumor-infiltrating lymphocytes / Jeroen Melief, Stina Wickstrom, Rolf Kiessling, and Yago Pico de Coana -- Expansion and determination of antigen-reactive T cells by flow cytometry / Alexander Martens, Graham Pawelec, and Christopher Shipp -- Assessing canonical and adaptive natural killer cell function in suppression assays in vitro / Dhifaf Sarhan and Jeffrey S. Miller -- Evaluating antibody-dependent cell-mediated cytotoxicity by chromium release assay / Irene van der Haar Ávila, Patricia Marmol, Rolf Kiessling, and Yago Pico de Coana -- Evaluating antibody-dependent cell-mediated cytotoxicity by flow cytometry / Irene van der Haar Ávila, Patricia Marmol, Jeannette Cany, Rolf Kiessling, and Yago Pico de Coana -- Evaluation of breast cancer and melanoma metastasis in syngeneic mouse models / Kristina Witt and Andreas Lundqvist -- Establishment of melanoma tumor xenograft using single cell line suspension and co-injection of patient-derived t cells in immune-deficient NSG mice / Fria̧ Bjork Gunnarsdottir, Rolf Kiessling, and Yago Pico de Coana -- Analysis of tissue-resident immune cells from mouse skin and lungs by flow cytometry / Felipe Galvez-Cancino, Ernesto Lopez, and Alvaro Lladser
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed January 22, 2019)
Subject Immunology -- Laboratory manuals
Immune system.
Immunologic Techniques
Immune System Phenomena
Cell Cycle Checkpoints
Medical -- Immunology.
Medical -- Oncology.
Immune system
Genre/Form Electronic books
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Pico de Coaña, Yago, editor
ISBN 9781493989799