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Title Animal cell biotechnology : methods and protocols / edited by Ralf Pörtner
Edition Fourth edition
Published New York, NY : Humana Press, 2020


Description 1 online resource (xiv, 399 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume 2095
Methods in molecular biology (Clifton, N.J.) ; v. 2095. 1064-3745
Summary Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed January 14, 2019)
Subject Animal cell biotechnology -- Laboratory manuals
Biotechnology -- methods
Cell Culture Techniques -- veterinary
Animal cell biotechnology
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Pörtner, Ralf, editor.
ISBN 9781071601914