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Num Mark Subjects (1-14 of 14) Year Entries
213 Found
1   Polymerase chain reaction -- 2 Related Subjects   2
2 Polymerase chain reaction   98
3 Polymerase chain reaction -- Automation : Microelectrofluidic systems : modeling and simulation / Tianhao Zhang, Krishnendu Chakrabarty, Richard B. Fair  2002 1
4 Polymerase chain reaction -- Diagnostic use.   17
5 Polymerase chain reaction -- Diagnostic use -- Handbooks, manuals, etc : PCR for clinical microbiology : an Australian and international perspective / edited by Margret Schuller [and others]  2010 1
6 Polymerase chain reaction -- Handbooks, manuals, etc : Handbook of HLA typing techniques / edited by Kam M. Hui, Jeffrey L. Bidwell  1993 1
7 Polymerase chain reaction -- Laboratory manuals.   36
8 Polymerase chain reaction -- Methodology.   12
9 Polymerase Chain Reaction -- methods.   40
10 Polymerase chain reaction -- Periodicals : Genome research (Online)    1
11 Polymerase Chain Reaction -- statistics & numerical data : PCR for clinical microbiology : an Australian and international perspective / edited by Margret Schuller [and others]  2010 1
12 Polymerase chain reaction -- Technique : Basic DNA and RNA protocols / edited by Adrian J. Harwood  1996 1
13 Polymerase Chain Reaction -- veterinary : Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications / Erika A. Pestana [and others]  2010 1
14  

Polymerase Chain Reactions -- See Polymerase Chain Reaction


In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships
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