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Title Minimal residual disease testing : current innovations and future directions / Todd E. Druley, editor
Published Cham : Springer, ©2019


Description 1 online resource (202 pages)
Contents Chapter 4: Monitoring AML Response Using "Difference from Normal" Flow CytometryIntroduction; Definition of Normal; Mutations Affect Phenotypic Expression; Specimen Composition and Quality; Lower Level of Detection; Performance Characteristics; Assay Validation; Direct Validation of [delta]N Post-HSCT; Correlative Validation of Response to Therapy; Indirect Validation to Monitoring Response to Therapy; MRD by Flow Cytometry Incorporated into Risk Stratification; References; Chapter 5: ML-DS: A Unique Condition for Measurable Residual Disease Detection; Introduction
Intro; Contents; Contributors; Chapter 1: Introduction; History of DNA Sequencing; Next-Generation Sequencing Application; Roche 454 Pyrosequencing; Illumina (Solexa) Sequencers; Sequencing by Oligonucleotide Ligation and Detection (SOLiD); Ion Torrent; DNA Nanoball Sequencing (DNBS); Third-Generation Sequencing; Single-Molecule Real Time (SMRT); Helicos Sequencing; Next-Generation Sequencing by Electron Microscopy; Fourth-Generation Sequencing; Nanopore Sequencing; BioNano Genomics; Emerging Platforms: Single-Cell Sequencing (SCS); References
Chapter 2: Minimal Residual Disease Testing in Acute Lymphoblastic Leukemia/LymphomaIntroduction; Minimal Residual Disease; Methods to Assess MRD; Flow Cytometry; Phenotypic Switch and Drug-Induced Immunophenotypic Modulation; Flow Cytometry Panels; Molecular PCR; Advantages and Disadvantages; Comparison of PCR and Flow Cytometry in Clinical Studies; Next-Generation Sequencing; NGS in T-ALL; NGS in B-ALL; Risk Stratification and the Threshold for MRD Detection; Does Therapeutic Intensification Overcome the Adverse Prognostic Effect of MRD?; Using MRD to Redefine Response
MRD as a Surrogate Marker of ResponseSummary; References; Chapter 3: Molecular Diagnostics for Minimal Residual Disease Analysis in Hematopoietic Malignancies; Introduction; RQ-PCR; BCR-ABL1; PML-RARA; CBF-AML (Core-Binding Factor AML); t(8; 21); RUNX1-RUNX1T1; Inv(16); MLL; WT1 Expression; BCL1 and BCL2 RQ-PCR; New Emerging and Patient-Specific; Flow Cytometric Cell Sorting (FACS); FACS Immunoglobulin (Ig) and T-Cell Receptor (TCR) Gene Rearrangements; FACS-FISH (Fluorescence In Situ Hybridization); NGS (Next-Generation Sequencing); AML; B- and T-Cell Malignancies; References
Minimizing Treatment-Related Toxicity: Early Treatment AdvancesMultidimensional Flow Cytometry in ML-DS Treatment and Residual Disease Monitoring; Making Sense of the Somatic Mutation Landscapes: Leveraging Error-Corrected NGS; Conclusions and Future Outlook; References; Chapter 6: Advancements in Next-Generation Sequencing for Detecting Minimal Residual Disease; Introduction; Cancer Genetics; History of DNA Sequencing; Next-Generation Sequencing Application; Bioinformatics and NGS; Quality Assessment of NGS Libraries; Genome Alignment and Processing; Variant Detection and Annotation
Summary This volume provides a concise yet comprehensive overview of minimal residual disease (MRD) testing. The text reviews the history of MRD testing, MRD testing for acute lymphoblastic leukemia/lymphoma, molecular diagnostics for MRD analysis in hematopoietic malignancies, the use of "difference from normal" flow cytometry in monitoring AML response, ML-DS for measurable residual disease detection, and advancements in next generation sequencing for detecting MRD. Written by experts in the field, Minimal Residual Disease Testing: Current Innovations and Future Directions is a valuable resource for hematologists, oncologists, pathologists, and radiologists on the variety of technologies available to detect MRD and how best to integrate these platforms into clinical practice
Notes Variant Filtering and Association Analyses
Includes index
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Diagnosis.
Molecular diagnosis.
Neoplasm, Residual -- diagnosis
Molecular Diagnostic Techniques -- methods
Flow Cytometry -- methods
High-Throughput Nucleotide Sequencing -- methods
HEALTH & FITNESS -- Diseases -- General.
MEDICAL -- Clinical Medicine.
MEDICAL -- Diseases.
MEDICAL -- Evidence-Based Medicine.
MEDICAL -- Internal Medicine.
Molecular diagnosis
Form Electronic book
Author Druley, Todd E
LC no. 2018957568
ISBN 9783319948270