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Title Manual of embryo culture in human assisted reproduction / edited by Kersti Lundin, Aisling Ahlström
Published Cambridge, United Kingdom ; New York, NY : Cambridge University Press, 2021


Description 1 online resource (vii, 116 pages) : illustrations (some color)
Series Cambridge laboratory manuals in assisted reproductive technology
Contents Facilities for embryo culture / Julius Hreinsson -- Incubators for embryo culture / Borut Kovačič -- Consumables for the IVF laboratory : production and validation, quality control and bioassays / Hubert Joris -- Embryo metabolism and what does the embryo need? Embryo stress / Paula Vergaro & Roger G. Sturmey -- Culture media and embryo culture / Arne Sunde & Roger G. Sturmey -- Optimal handling techniques for embryo culture / Michael Reed & Thomas Pool -- From identification to witnessing : traceability to ensure the safety of the embryo during culture / Kelly Tilleman & Annelies Tolpe -- Timing of embryo culture / Ioannis Sfontouris -- Time-lapse microscopy for embryo culture / Kirstine Kirkegaard & Thomas Freour -- Laboratory monitoring for embryo culture / Carlotta Zacà, Andrea Borini & Giovanni Coticchio -- Embryo culture and IVF offspring outcome / John C. Dumoulin & Aafke van Montfoort -- The changing culture of embryo culture : advances in the IVF culture system / Carole Curchoe & Jason E. Swain
Summary "In a span of just a couple of decades, human reproduction has been revolutionized by the widespread use of assisted reproduction. Many couples and individuals who previously could not achieve pregnancy and live birth are now able to fulfill their wish of a family. In addition, it is possible to postpone and plan the time for reproduction through improved cryopreservation techniques for gametes and embryos. Today, assisted reproduction techniques are established all over the world, having led to more than 8 million children born, with an estimated 10 million embryos cryopreserved. It is important to remember that the success of these - now more or less standard - procedures, have been accomplished by the dedicated work of a large number of scientists, embryologists and clinicians. However, despite considerable experimental and clinical research, we still only have a partial understanding of what constitutes a "true" embryo environment. In vivo the oocyte travels from the ovary to the uterus through a landscape of changing physiological conditions. Much effort has been made to mimic this varying environment in the in vitro culture but we still do not know whether in the end the embryos should be in utero for a better environment or maintained in vitro for a better selection. Special focus of research has been directed towards composition of the culture media, handling of the gametes and embryos, and to the design of specialized incubators to create stable and controlled conditions regarding pH, temperature and osmolarity"-- Provided by publisher
Bibliography Includes bibliographical references and index
Notes Online resource; title from digital title page (viewed on April 30, 2021)
Subject Fertilization in vitro, Human.
Fertilization in vitro.
Embryo Culture Techniques
Fertilization in Vitro
Fertilization in vitro.
Fertilization in vitro, Human.
Form Electronic book
Author Lundin, Kersti, editor.
Ahlström, Aisling, editor.
LC no. 2020042234
ISBN 9781108874014