Title Bioluminescent imaging : methods and protocols / edited by Christian E. Badr, Experimental Therapeutics and Molecular Imaging Laboratory, Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA, Program in Neuroscience, Harvard Medical School, Boston, MA, USA

Published New York : Humana Press, [2014] ©2014

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Description 1 online resource (xi, 276 pages) : illustrations (some color) Series Methods in molecular biology, 1940-6029 ; Volume No. 1098 Methods in molecular biology (Clifton, N.J.) ; v. 1098. 1064-3745 Contents Bioluminescence imaging : basics and practical limitations / Christian E. Badr -- Part I. In Vitro Biasassays -- Extraction and quantification of adenosine triphosphate in mammalian tissues and cells / Junji Chida and Hiroshi Kido -- Neuronal network imaging in acute slices using Ca² sensitive bioluminescent reporter / Ludovic Tricoire and Bertrand Lambolez -- Gaussia luciferase-based mycoplasma detection assay in mammalian cell culture / M. Hannah Degeling, M. Sarah S. Bovenberg, Marie Tannous, and Bakhos A. Tannous Part II. Imaging Molecular and Cellular Events -- Split gaussia luciferase for imaging ligand-receptor binding / Kathryn E. Luker and Gary D. Luker -- Video-rate bioluminescence imaging of protein secretion from a living cell / Takahiro Suzuki and Satoshi Inouye -- Bioluminescence reporter gene-based detection of microRNAs / Hae Young Ko, Young Sik Lee, and Soonhag Kim -- Monitoring of transcriptional dynamics of HIF and NFKB activities / Miguel A.S. Cavadas and Alex Cheong -- Real-time bioluminescent tracking of cellular population dynamics / Dan Close, Tingting Xu, Steven Ripp, and Gary Sayler -- Fabrication of bioluminescent capsules and live-cell imaging / Sung Bae Kim Part III. Imaging of Disease (Pathogenesis) -- The bioluminescent imaging of spontaneously occurring tumors in immunocompetent ODD-luciferase bearing transgenic mice / Scott J. Goldman and Shengkan Jin -- Blood-based assay with secreted gaussia luciferase to monitor tumor metastasis / Hiroshi Yamashita, Dan T. Nguyen, and Euiheon Chung -- Bioluminescence imaging of fungal biofilm development in live animals / Greetje Vande Velde, Soňa Kucharíková, Patrick Van Dijck, and Uwe Himmelreich -- Bioluminescent imaging of bacteria during mouse infection / Jonathan M. Warawa and Matthew B. Lawrenz Part IV. Therapeutic Imaging -- Cell-based bioluminescence screening assays / Romain J. Amante and Christian E. Badr -- Bioluminescence-based monitoring of virus vector-mediated gene transfer in mice / Casey A. Maguire -- Simultaneous in vivo monitoring of regulatory and effector T lymphocytes using secreted gaussia luciferase, firefly luciferase, and secreted alkaline phosphatase / Grant K. Lewandrowski, Ciara N. Magee, Marwan Mounayar, Bakhos A. Tannous, and Jamil Azzi Part V. Emerging Technologies -- Multiplex functional bioluminescent reporters using gaussia luciferase fused to epitope tags in an immunobinding assay / Sjoerd van Rijn, Thomas Würdinger, and Jonas Nilsson -- Noninvasive in vivo monitoring of extracellular vesicles / Charles P. Lai, Bakhos A. Tannous, and Xandra O. Breakefield -- In vitro and in vivo demonstrations of fluorescence by unbound excitation from luminescence (FUEL) / Joe Dragavon, Abdessalem Rekiki, Ioanna Theodorou, Chelsea Samson, Samantha Blazquez, Kelly L. Rogers, Régis Tournebize, and Spencer Shorte Summary Bioluminescent Imaging: Methods and Protocols distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology, neurology, infectious diseases, immunology, and others, the detailed chapters of this volume are arranged by topic and describe practical procedures and applications of different bioluminescent reporters, from photoproteins (Aequorin) to bacterial luciferases as well as other secreted (such as Gaussia) and non-secreted luciferases (such as Firefly). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols aims to provide diverse and comprehensive techniques to researchers interested in implementing bioluminescence-based imaging in their laboratory, regardless of their previous level of experience with such methodologies Bibliography Includes bibliographical references and index Notes English Print version record and online resource; title from PDF title page (Springer Protocols, viewed January 29, 2014) Subject Bioluminescence -- Laboratory manuals Imaging systems in biology -- Laboratory manuals Optical Imaging Bioluminescence Imaging systems in biology Biology. Health & Biological Sciences. Cytology. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Badr, Christian E., editor. ISBN 9781627037181 1627037187

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