Title Cell-penetrating peptides : methods and protocols / edited by Ülo Langel

Published New York : Humana, 2011

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Description 1 online resource (xv, 586 pages) : illustrations Series Methods in molecular biology, 1940-6029 ; 683 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 683. 1064-3745 Springer protocols (Series) Contents Classes and prediction of cell-penetrating peptides -- Penetratin story: An overview -- Testing membrane interactions of CPPs -- Interactions of amphipathic CPPs with model membranes -- NMR studies of three-dimensional structure and positioning of CPPs in membrane model systems -- Measurements of the intracellular stability of CPPs -- Tools for predicting binding and insertion of CPPs into lipid bilayers -- Studies of proteoglycan involvement in CPP-mediated delivery -- Uptake kinetics of cell-penetrating peptides -- Thermodynamics of lipid interactions with cell-penetrating peptides -- Calcium and membrane repair -- Mapping of protein transduction pathways with fluorescent microscopy -- Insight into cell-entry mechanisms of CPPs by electron microscopy -- Toxicity methods for CPPs -- Comparison of CPP uptake methods -- Characterization of cellular internalization pathways for CPP-mediated oligonucleotide delivery -- Mimicry of protein function with cell-penetrating peptides -- Homeoprotein intercellular transfer, the hidden face of cell-penetrating peptides -- Pharmacology, biodistribution, and efficacy of GPCR-based pepducins in disease models -- Identification and characterization of tissue-specific protein transduction domains using peptide phage display -- Applications of cell-penetrating peptides as signal transduction modulators for the selective induction of apoptosis -- Splice redirection as a convenient assay to monitor CPP-on efficiency and mechanism -- CPP-directed oligonucleotide exon skipping in animal models of Duchenne muscular dystrophy -- PTD-DRBD siRNA delivery -- A non-covalent peptide-based strategy for siRNA delivery -- Application of pepfect peptides for the delivery of splice-correcting oligonucleotides -- Internalization of nucleoside phosphates into live cells by complex formation with different CPPs and JBS-nucleoducin -- Enhanced cellular delivery of cell-penetrating peptide-peptide nucleic acid conjugates by photochemical internalization -- Identification of homing peptides using the in vivo phage display technology -- Measuring the action of CPP-siRNA conjugates in the lung -- Intracellular delivery of nanoparticles with CPPs -- Multifunctional CPP polymer system for tumor-targeted pDNA and siRNA delivery -- Cell-penetrating penta-peptides and bax-inhibiting peptides: Protocol for their application -- Pair technology: Exon-specific rna-binding protein isolation in live cells -- Quantitation of cellular and topical uptake of luciferin-oligoarginine conjugates -- Industrial-scale manufacturing of a possible oligonucleotide cargo CPP-based drug -- Application of a fusiogenic peptide GALA for intracellular delivery -- Therapeutic applications of cell-penetrating peptides -- Nonclinical and clinical experiences with CPP-based self-assembling peptide systems in topical drug development Summary In recent years, a new understanding of cell-penetrating peptides has emerged, helping researchers to expand beyond a number of long-held dogmas. In Cell-Penetrating Peptides: Methods and Protocols, expert researchers explore the latest information on cell-penetrating peptides (CPPs), providing insight into the most important and contemporary areas of CPP research. Chapters address the historical background of CPP studies, provide an overview of the growing field of research, investigate methods for testing CPP mechanisms, present a summary of methods that attempt to use properties of CPPs to study biochemical intracellular mechanisms of interaction and signal transduction, and include new ideas for turning CPP-based strategies into drugs. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and current, Cell-Penetrating Peptides: Methods and Protocols is an essential guide that allows researchers to study intracellular mechanisms in new ways, and to promote the future development of novel drugs Analysis celbiologie cellular biology levenswetenschappen life sciences Biology (General) Biologie (algemeen) Bibliography Includes bibliographical references and index Notes English Subject Peptides -- Physiological transport Drug carriers (Pharmacy) Metabolism. Life sciences. Drugs -- Dosage forms. Physical sciences. Drugs. Chemotherapy. Biological transport. Pharmacokinetics. Physiology. Therapeutics. Metabolism Biological Science Disciplines Drug Delivery Systems Dosage Forms Kinetics Publication Formats Peptides Publication Characteristics Biochemical Phenomena Natural Science Disciplines Pharmaceutical Preparations Specialty Uses of Chemicals Drug Therapy Amino Acids, Peptides, and Proteins Drug Carriers Laboratory Manuals Cell-Penetrating Peptides Biological Transport Pharmacokinetics Physiology Phenomena and Processes Therapeutics Chemical Phenomena Disciplines and Occupations Chemicals and Drugs Chemical Actions and Uses Analytical, Diagnostic and Therapeutic Techniques and Equipment Pharmaceutical Vehicles metabolism (biological concept) biological sciences. physical sciences. physiology. treating (health care function) Therapeutics Physiology Physical sciences Pharmacokinetics Metabolism Life sciences Drugs -- Dosage forms Drugs Chemotherapy Biological transport Drug carriers (Pharmacy) Peptides -- Physiological transport Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Langel, Ülo. ISBN 9781607619192 1607619199

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