Title Poly (ADP-ribose) polymerase : methods and protocols / edited by Alexei V. Tulin

Published New York : Humana : Springer, ©2011

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Description 1 online resource (xv, 521 pages) : illustrations Series Methods in molecular biology, 1940-6029 ; 780 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 780. 1064-3745 Springer protocols (Series) Contents Approaches to detect PARP-1 activation in vivo, in situ, and in vitro / Girish M. Shah [and others] -- Quantification of poly(ADP-ribose) in vitro : determination of the ADP-Ribose chain length and branching pattern / Rafael Alvarez-Gonzalez and Myron K. Jacobson -- Assay for protein modification by poly-ADP-Ribose in vitro / Opeyemi A. Olabisi and Chi-Wing Chow -- Identification of distinct amino acids as ADP-Ribose acceptor sites by mass spectrometry / Florian Rosenthal [and others] -- Noncovalent protein interaction with poly(ADP-Ribose) / Maria Malanga and Felix R. Althaus -- Noncovalent pADPr interaction with proteins and competition with RNA for binding to proteins / Yingbiao Ji -- Affinity-based assays for the identification and quantitative evaluation of noncovalent poly(ADP-Ribose)-binding proteins / Jean-Philippe Gagne [and others] -- Detecting and quantifying pADPr in vivo / Yi-Chen Lai [and others] -- Purification of recombinant poly(ADP-Ribose) polymerases / Jean-Christophe Ame [and others] -- Methods for purification of proteins associated with cellular poly(ADP-Ribose) and PARP-specific poly(ADP-Ribose) / Jennifer E. Rood, Anthony K.L. Leung, and Paul Chang -- Purification and characterization of poly(ADP-Ribosyl)ated DNA replication/repair complexes / Cynthia M. Simbulan-Rosenthal, Dean S. Rosenthal, and Mark E. Smulson -- PARP1 genomics : chromatin immunoprecipitation approach using anti-PARP1 antibody (ChIP and ChIP-seq) / Niraj Lodhi and Alexei V. Tulin -- Purification of human PARP-1 and PARP-1 domains from Escherichia coli for structural and biochemical analysis / Marie-France Langelier [and others] -- Regulation of chromatin structure by PARP-1 / Mi Young Kim -- Use of PARP inhibitors in cancer therapy : use as adjuvant with chemotherapy or radiotherapy ; use as a single agent in susceptible patients ; techniques used to identify susceptible patients / Sydney Shall [and others] -- Quantification of PARP activity in human tissues : ex vivo assays in blood cells and immunohistochemistry in human biopsies / Eszter M. Horvath, Zsuzsanna K. Zsengeller, and Csaba Szabo -- Assays for hypermethylation of the BRCA1 gene promoter in tumor cells to predict sensitivity to PARP-inhibitor therapy / Ilsiya Ibragimova and Paul Cairns -- Generation of poly(ADP-ribosyl)ation deficient mutants of the transcription factor, CTCF / Dawn Farrar, Igor Chernukhin, and Elena Klenova -- Phenotypic characterization of Parp-1 and Parp-2 deficient mice and cells / Christian Boehler [and others] -- Poly(ADP-ribosyl)ation pathways in mammals : the advantage of murine PARG null mutation / Yiran Zhou and David W. Koh -- Genetic approaches to targeting multiple PARP genes in a mammalian genome / Mirella L. Meyer-Ficca and Ralph G. Meyer -- Approaches to separate and identify polyADP-Ribosylated proteins using poly(ADP-Ribose) glycohydrolase-knockout Drosophila / Hiroto Nodono [and others] -- Immunolocalization of poly ADP-Ribose on Drosophila polytene chromosomes / Anna Sala and Davide F.V. Corona -- Immunodetection of pADPr in Drosophila tissues : immunostaining of thick sections; immuno-EM / Michal Jarnik -- Poly(ADP-Ribose) metabolism analysis in the Nematode Caenorhabditis elegans / Jean-Francois St-Laurent and Serge Desnoyers -- Genetic dissection of PARylation in the filamentous fungus Neurospora crassa / Gregory O. Kothe -- Purification of the poly-ADP-Ribose polymerase-like thermozyme from the Archaeon Sulfolobus solfataricus / Anna De Maio [and others] -- Strategies employed for the development of PARP inhibitors / Stacie Canan, Karen Maegley, and Nicola Curtin -- Small-molecule collection and high-throughput colorimetric assay to identify PARP1 inhibitors / Elena Kotova, Aaron D. Pinnola, and Alexei V. Tulin Summary Poly (ADP-ribose) Polymerases (PARPs) are abundant and ubiquitous proteins that regulate crucial processes of the cell cycle, DNA repair, genomic stability, and transcriptional regulation. Being involved in basic cell functions, PARPs mediate rapid responses to such environmental factors as stress, infection, nutrition and hormonal signals. Whereas PARP inhibitors can suppress tumor growth and proliferation in certain breast, ovarian, and prostate cancers, understanding how PARP controls cellular functions is essential for the development of novel cancer treatments strategies. Divided into three convenient sections, Poly(ADP-Ribose) Polymerase: Methods and Protocols aims to explain how PARP proteins act within the normal development of an organism as well as in pathogenic conditions, seeks to advance the knowledge of developmental pathways regulation, and endeavors to facilitate the development of new therapeutic drugs and methods to target PARP-dependent processes. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Poly(ADP-Ribose) Polymerase: Methods and Protocols serves as an ideal guide to scientists who wish to continue exploring this exciting and progressive research field Analysis levenswetenschappen life sciences celbiologie cellular biology eiwitexpressieanalyse proteomics Biology (General) Biologie (algemeen) Bibliography Includes bibliographical references and index Notes English Print version record Subject NAD-ADP-ribosyltransferase. Glycosyltransferases. Transferases. Enzymes. ADP Ribose Transferases Publication Characteristics Pentosyltransferases Glycosyltransferases Transferases Enzymes Enzymes and Coenzymes Chemicals and Drugs Poly(ADP-ribose) Polymerases Laboratory Manuals Publication Formats enzyme. Transferases Glycosyltransferases Enzymes NAD-ADP-ribosyltransferase Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Tulin, Alexei V. ISBN 9781617792700 1617792705

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