| Description |
1 online resource (xiv, 540 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1874 |
|
Methods in molecular biology (Clifton, N.J.) ; 1874. 1064-3745
|
| Contents |
Microinjection and micromanipulation : a historical perspective / Wenhao Xu -- Production of transgenic mice by pronuclear microinjection / Xin-an Pu, Anthony P. Young, and H. Michael Kubisch -- Transgene recombineering in bacterial artificial chromosomes / Michael G. Zeidler and Thomas L. Saunders -- Using TARGATT technology to generate site-specific transgenic mice / Ruby Yanru Chen-Tsai -- Generating genetically engineered mice Using a Spermatogonial Stem Cell-Mediated Method / Takuya Sato and Takehiko Ogawa -- Chimeric Mouse Generation by ES Cell Blastocyst microinjection and uterine transfer / Yubin Du, Wen Xie, Fan Zhang, and Chengyu Liu -- Creating knockin alleles in mouse embryonic stem cells by CRISPR/Cas9-mediated homologous recombination without drug selection / Pinghu Liu, Yan Li, Jingqi Lei, and Lijin Dong -- Using CRISPR/Cas9 for gene knockout in immunodeficient NSG mice / Yubin Du, Wen Xie, Fan Zhang, Uimook Choi, Chengyu Liu, and Colin L. Sweeney -- Generation of CRISPR-edited rodents using a piezo-driven zygote injection technique / Melissa A. Scott and Yueh-Chiang Hu -- Delivery of CRISPR-Cas9 into mouse zygotes by electroporation / Wenning Qin and Haoyi Wang -- Generation of conditional knockout mice by sequential insertion of two loxP sites in Cis using CRISPR/Cas9 and single-stranded DNA oligonucleotides / Ye Liu, Yubin Du, Wen Xie, Fan Zhang, Douglas Forrest, and Chengyu Liu -- Improvement of mouse cloning from any type of cell by nuclear injection / Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama -- CARD method for simple vitrification of mouse oocytes : advantages and applications / Naomi Nakagata, Jorge Sztein, and Toru Takeo -- CARD method for mouse sperm cryopreservation and in vitro fertilization using frozen-thawed sperm / Toru Takeo, Jorge Sztein, and Naomi Nakagata -- Isolation and analysis of a genome-edited single-hepatocyte from a Cas9 transgenic mouse line / Takayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B. Gurumurthy, Masahiro Sato, and Takayuki Shindo -- Microinjection and oviduct transfer procedures for rat model generation with CRISPR-Cas9 technology / Tuija M. Alcantar and Xin Y. Rairdan -- Molecular aspects of zinc finger nucleases (ZFNs)-Mediated gene editing in rat embryos / Xiaoxia Cui -- Organ generation from knockedout rat blastocysts complemented with pluripotent stem cells / Masumi Hirabayashi and Shinichi Hochi -- Generation of rabbit models by gene editing nucleases / Dongshan Yang, Jie Xu, and Y. Eugene Chen -- Production of genetically engineered porcine embryos by handmade cloning / Rong Li, Jinxin Miao, and Zhongde Wang -- Electrofusion of 2-cell embryos for porcine tetraploid embryo production / Qingran Kong and Zhonghua Liu -- Gene knockouts in goats using CRISPR/Cas9 system and somatic cell nuclear transfer / Zhiqiang Fan, Min Yang, Misha Regouski, and Irina A. Polejaeva -- Generating goat mammary gland bioreactors for producing recombinant proteins by gene targeting / Liyou An, Lan Yang, Yuejin Huang, Yong Cheng, and Fuliang Du -- Production of transgenic chickens using cultured primordial germ cells and gonocytes / Ellen J. Collarini, Philip A. Leighton, and Marie-Cecile Van de Lavoir -- Using microinjection to generate genetically modified Caenorhabditis elegans by CRISPR/Cas9 editing / Jyoti Iyer, Nicole DeVaul, Tyler Hansen, and Ben Nebenfuehr -- Microinjection in zebrafish for genome editing and functional studies / Wuhong Pei and Shawn M. Burgess -- Microinjection of marine fish eggs / Rie Goto, Taiju Saito, Takahiro Matsubara, and Etsuro Yamaha -- Generating gene knockout Oryzias latipes and rice field eel using TALENs method / Daji Luo, Ke Feng, Zuoyan Zhu, and Wei Hu -- Functional studies of transcriptional cofactors via microinjection-mediated gene editing in xenopus / Yuki Shibata, Lingyu Bao, Liezhen Fu, Bingyin Shi, and Yun-Bo Shi -- Microinjection of live mammalian cells : a delivery method that provides added versatility to the study of cellular function / Robert F. Gahl |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed October 24, 2018) |
| Subject |
Microinjections -- Laboratory manuals
|
|
Gene editing.
|
|
Microinjections -- methods
|
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Gene Editing
|
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Mice, Knockout
|
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Medical genetics.
|
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Biotechnology.
|
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Medical -- Genetics.
|
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Science -- Biotechnology.
|
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Gene editing
|
|
Microinjections
|
| Genre/Form |
Electronic books
|
|
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Liu, Chengyu, editor
|
|
Du, Yubin, editor
|
| ISBN |
9781493988310 |
|
149398831X |
|
1493988301 |
|
9781493988303 |
|
