| Description |
1 online resource (xi, 257 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; 633 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 633. 1064-3745
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Springer protocols (Series)
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| Contents |
The culture of mouse embryonic stem cells and formation of embryoid bodies / Melany Jackson [and others] -- Derivation of primary mouse embryonic fibroblast (PMEF) cultures / Alastair S. Garfield -- Embryonic skeletal muscle microexplant culture and isolation of skeletal muscle stem cells / Janet Smith and Deborah Merrick -- The embryonic kidney : isolation, organ culture, immunostaining, and RNA interference / Jamie A. Davies -- Explant culture of mouse embryonic whole lung, isolated epithelium, or mesenchyme under chemically defined conditions as a system to evaluate the molecular mechanism of branching morphogenesis and cellular differentiation / Pierre-Marie del Moral and David Warburton -- Isolation, culture, and characterisation of mouse embryonic oesophagus and intestine / Jonathan M. Quinlan, Wei-Yuan Yu, and David Tosh -- Isolation and culture of embryonic pancreas and liver / Zoë D. Burke [and others] -- Isolation and culture of mouse satellite cells / Antonio Musarò and Laura Barberi -- Analysis of cardiac myocyte biology in transgenic mice : a protocol for preparation of neonatl mouse cardiac myocyte cultures / Nigel J. Brand [and others] -- Short- and long-term cultivation of embryonic and neonatal murine keratinocytes / Reto Caldelari and Eliane J. Müller -- Isolation, culture, and analysis of mouse mammary epithelial cells / Matthew J. Smalley -- Isolation and culture of mouse pancreatic islets for ex vivo imaging studies with trappable or recombinant fluorescent probes / Magalie A. Ravier and Guy A. Rutter -- Isolation and culture of adult mouse hepatocytes / Wan-Chun Li, Kate L. Ralphs, and David Tosh -- Isolation and culture of mouse intestinal cells / Charles Frederick Campbell -- Derivation of primary choroid plexus epithelial cells from the mouse / Trevelyan R. Menheniott, Marika Charalambous, and Andrew Ward -- The preparation of primary cortical neuron cultures and a practical application using immunofluorescent cytochemistry / Carla Sciarretta and Liliana Minichiello -- Cell culture of primary cerebellar granule cells / Dana Kramer and Liliana Minichiello -- Isolation and generation of neurosphere cultures from embryonic and adult mouse brain / Henrik Ahlenius and Zaal Kokaia |
| Summary |
Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders |
| Analysis |
celbiologie |
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cellular biology |
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celkweek |
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cell culture |
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diermodellen |
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animal models |
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levenswetenschappen |
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life sciences |
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Biology (General) |
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Biologie (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Mice -- Cytology -- Laboratory manuals
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Cell culture -- Laboratory manuals
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Mice.
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Cell Culture Techniques -- veterinary
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Mice
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Mice
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Cell culture
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Mice -- Cytology
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Ward, Andrew, 1964-
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|
Tosh, David
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| ISBN |
9781597450195 |
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1597450197 |
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