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Title FOXO transcription factors : methods and protocols / edited by Wolfgang Link
Published New York, N.Y. : Humana Press : Springer, [2018]
©2019

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Description 1 online resource (xii, 270 pages) : illustrations (some color)
Series Methods in Molecular Biology ; v. 1890
Methods in molecular biology (Clifton, N.J.) ; v. 1890. 1064-3745
Contents Introduction to FOXO biology / Wolfgang Link -- Characterization of MicroRNAs regulating FOXO expression / Doug Hanniford and Eva Hernando -- Identification of transcription factor-binding sites in the mouse FOXO1 promoter / Christopher P. Cardozo -- Investigating mechanisms that control ubiquitin-mediated DAF-16/FOXO protein turnover / Thomas Heimbucher and Coleen T. Murphy -- Phosphorylation of FOXO proteins as a key mechanism to regulate their activity / Alba Orea-Soufi, David Davila, Maria Salazar-Roa, Maria de Mar Lorente, and Guillermo Velasco -- Methodological approach for the evaluation of FOXO as a positive regulator of antioxidant genes / Maria Monsalve, Ignacio Prieto, Andreza Fabro de Bem, and Yolanda Olmos -- Characterization of FOXO acetylation / Shang Yao, Zimam Mahmud, Nikoleta Sachini, Sathid Aimjongjun, Paula Saavedra-Garcia, and Eric W-F Lam -- Transcriptional activity of FOXO transcription factors measured by luciferase assays / Antonia Avila-Flores, Javier Arranz-Nicolas, and Isabel Merida -- Monitoring the transcriptional activity of FOXO transcription factors by analyzing their target genes / Vanessa Henriques, Susana Machado, Wolfgang Link, and Bibiana I. Ferreira -- Using ChIP-based approaches to characterize FOXO recruitment to its target promoters / Neeraj Kumar and Arnab Mukhopadhyay -- RNAi-mediated silencing of FOXO factors / Priyankar Sanphui, Akash Saha, and Subhas Chandra Biswas -- Immunofluorescence analysis by confocal microscopy for detecting endogenous FOXO / Francisco Castillo, Thomas A. Mackenzie, and Bastien Cautain -- High-throughput image-based screening to identify chemical compounds capable of activating FOXO / Marta Barradas, Wolfgang Link, Diego Megias, and Pablo J. Fernandez-Marcos -- Image-based identification of chemical compounds capable of trapping FOXO in the cell nucleus / Susana Machado, Catarina Raposo, Bibiana I. Ferreira, and Wolfgang Link -- Quantifying tissue-specific overexpression of FOXO in Drosophila via mRNA fluorescence in situ hybridization using branched DNA probe technology / Anna C. Blice-Baum, Georg Vogler, Meera C. Viswanathan, Bosco Trinh, Worawan B. Limpitikul, and Anthony Cammarato -- Genome-wide analysis for identifying FOXO protein-binding sites / Dong-Ju Shin, Pujan Joshi, Dong-Guk Shin, and Li Wang -- Mathematical modeling of nuclear trafficking of FOXO transcription factors / Bradford E. Peercy and Martin F. Schneider -- Following transcriptome to uncover FOXO biological functions / Raymond Liang, Vijay Menon, and Saghi Ghaffari -- Hydra as model to determine the role of FOXO in longevity / Thomas C.G. Bosch -- Genetic ablation of FOXO in mice to investigate its physiological role / Hwanhee Oh and Jihye Paik -- Analysis of FOXO3 gene polymorphisms associated with human longevity / Timothy A. Donlon, Philip M.C. Davy, and Bradley J. Willcox -- Analysis of FOXO3a gene polymorphism associated with asthma / Tejas Shah and Kalpana Joshi
Notes Online resource; title from PDF title page (SpringerLink, viewed November 12, 2018)
Subject Transcription factors -- Laboratory manuals
Transcription factors.
Transcription Factors
Proteins.
Science -- Life Sciences -- Biochemistry.
Transcription factors
Genre/Form Electronic books
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Link, Wolfgang, editor
ISBN 9781493989003
1493989006