| Description |
1 online resource (355 p.) |
| Series |
Advances in experimental medicine and biology ; v. 1407 |
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Advances in experimental medicine and biology ; v. 1407.
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| Contents |
Intro -- Preface -- Contents -- Editor and Contributors -- Chapter 1: Pseudotyped Viruses -- 1.1 Introduction -- 1.2 The Vectors for Enveloped Viruses -- 1.2.1 Lentiviral Vectors for Enveloped Viruses -- 1.2.1.1 HIV Vector -- 1.2.1.2 Murine Leukemia Virus Vector -- 1.2.1.3 FIV Vector -- 1.2.2 The Vesicular Stomatitis Virus Vector -- 1.2.2.1 VSV Reverse Genetics -- 1.2.2.2 Recombinant VSV Vectors -- 1.3 Self-Assembled Constructed Pseudotyped Viruses -- 1.3.1 Self-Assembled Pseudotyped Virus for Enveloped Viruses -- 1.3.2 Self-Assembled Pseudotyped Virus for Non-enveloped Viruses |
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1.4 The Parameters and Conditions for Construction and Package of Pseudotyped Viruses -- 1.4.1 Viral Biological Characteristic Affects Pseudotyped Virus Formation and Titer -- 1.4.2 The Effects of Envelope Protein Expression -- 1.4.3 The Effects of Packaging System -- 1.4.4 Effects of Proteases -- 1.4.5 Selection of Cell Lines for Pseudovirus Packaging and Detection -- 1.4.6 The Effect of Packaging Conditions -- References -- Chapter 2: Assays Based on Pseudotyped Viruses -- 2.1 Introduction -- 2.2 Development of In Vitro Assays Based on Pseudotyped Viruses |
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2.2.1 Establishment and Optimization of In Vitro Assays Based on Pseudotyped Viruses -- 2.2.1.1 Selection of Target Cells -- 2.2.1.2 Optimization of Cell Inoculation -- 2.2.1.3 Optimization of the Amount of Pseudotyped Virus -- 2.2.1.4 Optimization of Infection Conditions -- 2.2.1.5 Optimization of Culture Time -- 2.2.1.6 Validation of In Vitro Assays Based on Pseudotyped Viruses -- 2.2.1.7 Specificity -- 2.2.1.8 Accuracy -- 2.2.1.9 Linear Range -- 2.2.1.10 Precision -- 2.3 Development of In Vivo Assays Based on Pseudotyped Viruses -- 2.3.1 Selection of Model Animals |
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2.3.2 Optimization of Infection Pathway -- 2.3.3 Determination of Infection Dose of Pseudotyped Virus -- 2.3.4 Comparison of Pseudotyped and Live Virus Infection Model -- 2.4 Conclusion -- References -- Chapter 3: Application of Pseudotyped Viruses -- 3.1 Analysis of Viral Infectivity -- 3.1.1 Receptor Usage -- 3.1.2 Cellular Tropism -- 3.2 In Vitro Pseudovirion-Based Neutralization Assay (PBNA) -- 3.2.1 Development and Evaluation of Vaccines -- 3.2.2 Screening and Validation of Monoclonal Neutralizing Antibodies -- 3.3 Screening and Validation of Viral Entry Inhibitors |
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3.4 Animal Model of Pseudotyped Virus Infection In Vivo -- 3.5 Analysis of Variations in Viral Infectivity and Antigenicity -- 3.5.1 Viral Variants -- 3.5.2 Variations in Viral Glycosylation -- 3.6 Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) -- 3.7 Conclusion -- References -- Chapter 4: Pseudotyped Viruses for Retroviruses -- 4.1 Introduction to Retroviruses -- 4.2 The Retrovirus Genome and Replication Cycle: Important Regions for Retroviral Vectors -- 4.2.1 Genome Structure -- 4.2.2 Retroviral Proteins -- 4.2.3 Viral RNA Replication -- 4.3 Retroviral Pseudotyping Systems |
| Summary |
This book intends to report the new progress of pseudotyped viruses, including the construction of pseudotyped viruses with different strategies or vectors for most important viruses. Especially for emerging viruses, optimization of the condition and parameters for assay development based on the pseudotyped viruses and widely application as surrogate of authentic virus to study the biological functions of virus, detection of neutralizing antibody, screening viral entry inhibiters, and others. It includes most pseudotyped viruses that have the protein of the target virus on the surface of the parent virus with incomplete genome. The book is likely to be of interest to all researchers in the field of virology, vaccine, and anti-viral drug development and evaluation |
| Notes |
4.3.1 Overview of Retroviral Pseudotyping Plasmid Systems |
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Description based on online resource; title from digital title page (viewed on August 10, 2023) |
| Subject |
Recombinant viruses.
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Recombinant viruses
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| Form |
Electronic book
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| Author |
Wang, Youchun, 1964- editor.
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| ISBN |
9789819901135 |
|
9819901138 |
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