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Book Cover
E-book

Title Germ cell protocols. Part 1, Sperm and oocyte analysis / edited by Heide Schatten
Published Totowa, N.J. : Humana Press, ©2004

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Description 1 online resource (1 volume) : illustrations (some color)
Series Methods in molecular biology ; v. 253, pt. 1
Methods in molecular biology (Clifton, N.J.) ; volume 253, pt. 1
Contents Cryopreservation of semen of the salmonidae with special reference to large-scale fertilization / F. Lahnsteiner -- Fertilization and sperm chemotaxis in ascidians / M. Yoshida -- Assays for vertebrate sperm chemotaxis / H. Sugiyama [and others] -- Stallion spermatozoa viability: Comparison of flow cytometry with other methods / K. Merkies and M.M. Buhr -- Visualization of sperm accessory structures in the mammalian spermatids, spermatozoa, and zygotes by immunofluorescence, confocal, and immunoelectron microscopy / P. Sutovsky -- Localization of zona pellucida receptors on live sperm by fluorophore-conjugated solubilized zona pellucida proteins / H.R. Burkin, A. Paula Alves-Vieira and D.J. Miller -- Detection of human papillomavirus DNA in sperm using polymerase chain reaction / O.A. Olatunbosun, A.M. Case and H.G. Deneer -- Spermatogonial stem cells / K. Nayernia, M. Li and W. Engel -- Molecular and cellular approaches for establishment of an in vitro spermatogenesis system / K. Nayernia and W. Engel -- Acrosin activity assay for the evaluation of mammalian sperm acrosome reaction / Y. Lax, S. Rubinstein and H. Breitbart -- Isolation of sea urchin sperm plasma membranes / K.J. Mengerink and V.D. Vacquier -- Detection of centrosome structure in fertilized and artificially activated sea urchin eggs using immunofluorescence microscopy and isolation of centrosomes followed by structural characterization with field emission scanning electron microscopy / H. Schatten and A. Chakrabarti -- Microscopic techniques for studying sperm-oocyte interaction during fertilization and early embryonic development / M.W. Tengowski -- Immunocytochemical staining of the metaphase ii spindle in mammalian oocytes / S.F. Mullen and J.K. Critser -- Spindle imaging in living mammalian oocytes using the liquid-crystal polarized light microscope and its practical use / W.H. Wang, J. Gill and C. Boutin -- In vitro maturation and fertilization of pig oocytes / H.Y. Fan and Q.Y. Sun -- In vitro maturation and in vitro fertilization of human oocytes / W.H. Wang and J. Gill -- In vitro maturation and fertilization of canine oocytes / K. Reynaud, M. Saint-Dizier and S. Chastant-Maillard -- Detection and measurement of membrane-bound protein tyrosine kinases in the zebrafish egg / W. Wu and W.H. Kinsey -- Single-cell method for estimation of protein tyrosine kinases in the zebrafish egg / E. MacGregor and W.H. Kinsey -- Activity of MAPK/p90rsk during fertilization in mice, rats, and pigs / Q.Y. Sun and H.Y. Fan
Summary A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Highlights include in vitro maturation and fertilization of human, porcine, and canine oocytes; the cryopreservation of sperm cells; establishment of an in vitro spermatogenesis system; visualization of sperm accessory structures; and motility assays of stallion spermatozoa. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls
Analysis kiemcellen
germ cells
spermatozoa
oocytes
laboratoriummethoden
laboratory methods
technieken
techniques
rijpen
maturation
in vitro bevruchting
in vitro fertilization
genetische analyse
genetic analysis
celbiologie
cellular biology
moleculaire biologie
molecular biology
protocollen
protocols
Reproduction and Fertility
Cellular Biology
Voortplanting en vruchtbaarheid
Celbiologie
Bibliography Includes bibliographical references and indexes
Notes Print version record
Subject Veterinary embryology -- Research -- Technique
Germ cells -- Research -- Technique
Cytology -- Technique.
Germ cells.
Genetics -- Technique.
Cytological Techniques
Germ Cells
Genetic Techniques
MEDICAL -- Veterinary Medicine -- Food Animal.
Cytology -- Technique
Genetics -- Technique
Germ cells
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Schatten, Heide.
ISBN 1592597440
9781592597444
Other Titles Sperm and oocyte analysis