Description |
1 online resource (1 volume) : illustrations (some color) |
Series |
Methods in molecular biology ; v. 253, pt. 1 |
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Methods in molecular biology (Clifton, N.J.) ; volume 253, pt. 1
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Contents |
Cryopreservation of semen of the salmonidae with special reference to large-scale fertilization / F. Lahnsteiner -- Fertilization and sperm chemotaxis in ascidians / M. Yoshida -- Assays for vertebrate sperm chemotaxis / H. Sugiyama [and others] -- Stallion spermatozoa viability: Comparison of flow cytometry with other methods / K. Merkies and M.M. Buhr -- Visualization of sperm accessory structures in the mammalian spermatids, spermatozoa, and zygotes by immunofluorescence, confocal, and immunoelectron microscopy / P. Sutovsky -- Localization of zona pellucida receptors on live sperm by fluorophore-conjugated solubilized zona pellucida proteins / H.R. Burkin, A. Paula Alves-Vieira and D.J. Miller -- Detection of human papillomavirus DNA in sperm using polymerase chain reaction / O.A. Olatunbosun, A.M. Case and H.G. Deneer -- Spermatogonial stem cells / K. Nayernia, M. Li and W. Engel -- Molecular and cellular approaches for establishment of an in vitro spermatogenesis system / K. Nayernia and W. Engel -- Acrosin activity assay for the evaluation of mammalian sperm acrosome reaction / Y. Lax, S. Rubinstein and H. Breitbart -- Isolation of sea urchin sperm plasma membranes / K.J. Mengerink and V.D. Vacquier -- Detection of centrosome structure in fertilized and artificially activated sea urchin eggs using immunofluorescence microscopy and isolation of centrosomes followed by structural characterization with field emission scanning electron microscopy / H. Schatten and A. Chakrabarti -- Microscopic techniques for studying sperm-oocyte interaction during fertilization and early embryonic development / M.W. Tengowski -- Immunocytochemical staining of the metaphase ii spindle in mammalian oocytes / S.F. Mullen and J.K. Critser -- Spindle imaging in living mammalian oocytes using the liquid-crystal polarized light microscope and its practical use / W.H. Wang, J. Gill and C. Boutin -- In vitro maturation and fertilization of pig oocytes / H.Y. Fan and Q.Y. Sun -- In vitro maturation and in vitro fertilization of human oocytes / W.H. Wang and J. Gill -- In vitro maturation and fertilization of canine oocytes / K. Reynaud, M. Saint-Dizier and S. Chastant-Maillard -- Detection and measurement of membrane-bound protein tyrosine kinases in the zebrafish egg / W. Wu and W.H. Kinsey -- Single-cell method for estimation of protein tyrosine kinases in the zebrafish egg / E. MacGregor and W.H. Kinsey -- Activity of MAPK/p90rsk during fertilization in mice, rats, and pigs / Q.Y. Sun and H.Y. Fan |
Summary |
A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Highlights include in vitro maturation and fertilization of human, porcine, and canine oocytes; the cryopreservation of sperm cells; establishment of an in vitro spermatogenesis system; visualization of sperm accessory structures; and motility assays of stallion spermatozoa. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls |
Analysis |
kiemcellen |
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germ cells |
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spermatozoa |
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oocytes |
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laboratoriummethoden |
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laboratory methods |
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technieken |
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techniques |
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rijpen |
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maturation |
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in vitro bevruchting |
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in vitro fertilization |
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genetische analyse |
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genetic analysis |
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celbiologie |
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cellular biology |
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moleculaire biologie |
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molecular biology |
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protocollen |
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protocols |
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Reproduction and Fertility |
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Cellular Biology |
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Voortplanting en vruchtbaarheid |
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Celbiologie |
Bibliography |
Includes bibliographical references and indexes |
Notes |
Print version record |
Subject |
Veterinary embryology -- Research -- Technique
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Germ cells -- Research -- Technique
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Cytology -- Technique.
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Germ cells.
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Genetics -- Technique.
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Cytological Techniques
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Germ Cells
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Genetic Techniques
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MEDICAL -- Veterinary Medicine -- Food Animal.
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Cytology -- Technique
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Genetics -- Technique
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Germ cells
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Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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Form |
Electronic book
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Author |
Schatten, Heide.
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ISBN |
1592597440 |
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9781592597444 |
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