| Description |
1 online resource (xi, 390 pages) : illustrations |
| Series |
Springer protocols |
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Methods in molecular biology, 1940-6029 ; v. 586 |
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Springer protocols (Series)
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Methods in molecular biology (Clifton, N.J.) ; v/ 586. 1064-3745
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| Contents |
High resolution multimode light microscopy of cell migration: long-term imaging and analysis / T. Wollert and G.M. Langford -- Imaging the cytoskeleton in live Xenopus laevis embryos / S. Woolner, A.L. Miller and W.M. Bement -- Live-cell imaging of the cytoskeleton and mitochondrial-cytoskeletal interactions in budding yeast / T.C. Swayne, T.G. Lipkin and L.A. Pon -- Jasplakinolide: an actin-specific reagent that promotes actin polymerization / A. Holzinger -- Analysis of centrosome function and microtubule dynamics by time-lapse microscopy in Xenopus egg extracts / C. Wiese, J.R. Mayers and A.J. Albee -- Quantitative fluorescence microscopy techniques / A. Esposito [and others] -- A FRET-based approach for studying conformational changes of a cytoskeleton-related tumor suppressor molecule / R.F. Hennigan [and others] -- Sample preparation for fluorescence imaging of the cytoskeleton in fixed and living plant roots / J. Dyachok [and others] -- Imaging of the cytoskeleton and mitochondria in fixed budding yeast cells / T.C. Swayne, I.R. Boldogh and L.A. Pon -- Imaging cytoskeleton components by electron microscopy / T. Svitkina -- Purification and localization of intraflagellar transport particles and polypeptides / R.D. Sloboda -- High pressure freezing, electron microscopy, and immuno-electron microscopy of Tetrahymena thermophila basal bodies / J.B. Meehl, T.H. Giddings, Jr. and M. Winey -- Strategies for imaging microtubules in plant cells / A. Holzinger, E. Kawamura and G.O. Wasteneys -- A rapid tracking method for the quantitative analysis of organelle streaming velocity / C.L. Holweg -- Melanosome motility in fish retinal pigment epithelial cells / C. King-Smith -- Analysis of properties of cilia using Tetrahymena thermophila / V. Rajagopalan [and others] -- Application of cell traction force microscopy for cell biology research / J.H. Wang and B. Li -- 2D and 3D quantitative analysis of cell motility and cytoskeletal dynamics / D. Wessels, S. Kuhl and D.R. Soll -- Detergent-extracted models for the study of cilia or flagella / C.B. Lindemann and K.A. Lesich -- The analysis of intermediate filament dynamics using transfections and cell fusions / J.M. Paramio -- Functional analysis of actin-binding proteins in the central nervous system of Drosophila / Q. He -- Proteomic tools for the analysis of cytoskeleton proteins / E. Wywial, V.N. Dongre and S.M. Singh |
| Summary |
In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of components. In Cytoskeleton Methods and Protocols, Second Edition, internationally renowned experts present techniques which reflect many of the recent technological advances in experimental tools for cytoskeleton research with emphasis on animal, plant, protist, and fungal model systems. This cutting-edge volume contains methods for live-cell imaging, fluorescence microscopy, electron microscopy, analysis of cell and organelle motility, isolation of cytoskeleton components, and proteomics, amongst other topics. As a volume in the highly successful Methods in Molecular Biology"!series, chapters incorporate introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes that provide unpublished technical information on troubleshooting and avoiding known pitfalls. Up-to-date and comprehensive, Cytoskeleton Methods and Protocols, Second Edition serves as an ideal guide to scientists who wish to continue this fruitful and important biological research |
| Analysis |
celskelet |
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cytoskeleton |
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celstructuur |
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cell structure |
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microscopie |
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microscopy |
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methodologie |
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methodology |
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biologische technieken |
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biological techniques |
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protocollen |
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protocols |
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Biological Techniques |
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Biologische technieken |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| Subject |
Cytoskeleton -- Laboratory manuals
|
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Cytoskeletal proteins -- Laboratory manuals
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Cytoskeleton
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Cystoskeletal Proteins
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Cytoskeletal proteins
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Cytoskeleton
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Gavin, Ray H.
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| LC no. |
2009930645 |
| ISBN |
9781607613763 |
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160761376X |
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