| Description |
1 online resource (xiv, 373 pages) : illustrations (chiefly color) |
| Series |
Methods in molecular biology, 1940-6029 ; 2565 |
|
Methods in molecular biology (Clifton, N.J.) ; 2565. 1940-6029
|
| Contents |
Rat pheochromocytoma PC12 cells in culture -- Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantation -- Immunocytochemistry of acutely isolated adrenal medullary chromaffin cells -- Transmission electron microscopy: a method for studying the adrenochromaffin cells -- Immunogold for protein location in chromaffin cells -- Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopy -- Online detection of catecholamine release from the perfused rat adrenal gland -- On-line recording of perifused chromaffin cells -- Recording of chromaffin cell electrical activity in situ in acute adrenal slices -- Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive Rats -- Measurements of calcium in chromaffin cell organelles using targeted aequorins -- Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurements -- Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cell -- Quantal release analysis of electrochemically active molecules using single-cell amperometry -- Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAs -- Vesicle collision protocols for the study of quantum size and exocytotic fraction released -- Patch Amperometry and Intracellular Patch Electrochemistry -- Artificial cells for dissecting exocytosis -- Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cells -- Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells -- Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging -- Determination of catecholamines in a small volume (25[mu]l) of plasma from conscious mouse tail vein -- Quantification of chromogranin A and its fragments in biological fluids -- Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds |
| Summary |
This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. |
| Notes |
Includes index |
|
Online resource; title from PDF title page (SpringerLink, viewed October 13, 2022) |
| Subject |
Chromaffin cells.
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|
Chromaffin cells
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Borges, Ricardo, editor
|
| ISBN |
9781071626719 |
|
107162671X |
|
