| Description |
1 online resource (xiii, 431 pages) : illustrations |
| Series |
Springer protocols |
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Methods in molecular biology, 1940-6029 ; v. 1365 |
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Springer protocols (Series)
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Methods in molecular biology (Clifton, N.J.) ; v. 1365. 1064-3745
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| Contents |
Imaging the cytoskeleton in live and fixed cells. Long-term live cell imaging of cell migration : effects of pathogenic fungi on human epithelial cell migration / Torsten Wöllert and George M. Langford -- Live-cell imaging of mitochondria and the actin cytoskeleton in budding yeast / Ryo Higuchi-Sanabria, Theresa C. Swayne, Istvan R. Boldogh, and Liza A. Pon -- Imaging of the actin cytoskeleton and mitochondria in fixed budding yeast cells / Ryo Higuchi-Sanabria, Theresa C. Swayne, Istvan R. Boldogh, and Liza A. Pon -- Imaging of the cytoskeleton using live and fixed Drosophila tissue culture cells / Derek A. Applewhite, Christine A. Davis, Eric R. Griffis, and Omar A. Quintero -- Imaging cytoskeleton components by electron microscopy / Tatyana Svitkina -- Purification and localization of intraflagellar transport particles and polypeptides / Roger D. Sloboda -- Fluorescence imaging of the cytoskeleton in plant roots / Julia Dyachok, Ana Paez-Garcia, Cheol-Min Yoo, Karuppaiah Palanichelvam, and Elison B. Blancaflor -- Microtubules in plant cells : strategies and methods for immunofluorescence, transmission electron microscopy, and live cell imaging / Katherine Celler, Miki Fujita, Eiko Kawammura, Chris Ambrose, Klaus Herburger, Andreas Holzinger, and Geoffrey O. Wasteneys |
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Cytoskeleton dynamics. Basic methods to visualize actin filaments in vitro using fluorescence microscopy for observation of filament severing and bundling / Shoichiro Ono -- An in vitro model system to test mechano-microbiological interactions between bacteria and host cells / Luís Carlos Santos, Emilia Laura Munteanu, and Nicolas Biais -- Reconstitution of a minimal actin cortex by coupling actin filaments to reconstituted membranes / Sven K. Vogel -- Use of nanobodies to localize endogenous cytoskeletal proteins and to determine their contribution to cancer cell invasion by using an ECM degradation assay / Isabel Van Audenhove and Jan Gettemans -- Actin-dynamics in plant cells : the function of actin-perturbing substances : Jasplakinolide, Chondramides, Phalloidin, Cytochalasins and Latrunculins / Andreas Holzinger and Kathrin Blaas |
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Cell and organelle motility. Quantitative motion analysis in two and three dimensions / Deborah J. Wessels, Daniel F. Lusche, Spencer Kuhl, Amanda Scherer, Edward Voss, and David R. Soll -- Measurement of cell motility using microgrooved substrates / James H.-C. Wang, Guangyi Zhao, and Bin Li -- The study of cell motility by cell traction force microscopy (CTFM) / James H.-C. Wang, Guangyi Zhao, and Bin Li -- Melanosome motility in fish retinal pigment epithelial (RPE) cells / Christina King-Smith -- Analysis of stem cell motility in vivo based on ommunodetection of planarian neoblasts and tracing of BrdU-labeled cells after partial irradiation / Junichi Tasaki, Chichiro Uchiyama-Tasaki, and Labib Rouhana -- Chemotaxis : under agarose assay / Derrick Brazill |
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Genetic and proteomic protocols. Functional analysis of actin-binding proteins in the central nervous system of Drosophila / Qi He and Christopher Roblodowski -- Proteomic analysis of cytoskeleton proteins in fish / Michael Gotesman, Simon Menanteau-Ledouble, and Mansour El-Mabouli -- Using a handheld gene gun for genetic transformation of Tetrahymena thermophila / Michael Gotesman and Selwyn A. Williams -- Proteomic tools for the analysis of cytoskeleton proteins / Michael Scarpati, Mary Ellen Heavner, Eliza Wiech, and Shaneen Singh -- Homology modeling procedures for cytoskeletal proteins of Tetrahymena and other ciliated protists / Giovanni J. Pagano, Linda A. Hufnagel, and Roberta S. King |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Cytoskeleton -- Laboratory manuals
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Cytoskeletal proteins -- Laboratory manuals
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Cytoskeletal proteins.
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Cytoskeleton
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Cytoskeletal Proteins
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Cellular biology (cytology)
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Science -- Life Sciences -- Biology -- Molecular Biology.
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Cytoskeletal proteins
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Cytoskeleton
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Gavin, Ray H.
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| LC no. |
2015947776 |
| ISBN |
9781493931248 |
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1493931245 |
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