| Description |
1 online resource (x, 278 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 90 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 90
|
| Contents |
DNase I footprinting / Keith R. Fox -- Quantitative DNA footprinting / James C. Dabrowiak, Jerry Goodisman, Brian Ward -- Uranyl photoprobing of DNA Structures and drug-DNA complexes / Niels Erik Møllegaard, Peter E. Nielsen -- Diethylpyrocarbonate and osmium tetroxide as probes for drug-induced changes in DNA conformation in vitro / Christian Bailly, Michael J. Waring -- Footprinting studies with nucleosome-bound DNA / Philip M. Brown, Keith R. Fox -- A gel mobility shift assay for probing the effect of drug-DNA adducts on DNA-binding proteins / Suzanne M. Cutts, Andrew Masta, Con Panousis, Peter G. Parsons, Richard A. Sturm, Don R. Phillips -- An oligonucleotide crosslinking assay for the analysis of individual drug-binding sites / Suzanne M. Cutts, Con Panousis, Andrew Masta, Don R. Phillips -- DNA polymerase inhibition assay (PIA) for the detection of drug-DNA interactions / Thomas P. Dooley, Katherine L. Weiland -- Transcriptional footprinting of drug-DNA interactions / Don R. Phillips, Carleen Cullinane -- Determination of the DNA sequence specificity of alkylation damage using cleavage-based assays / John A. Hartley, Michael D. Wyatt -- PCR-based methods for detecting DNA damage and its repair at the subgene and single nucleotide levels in cells / Keith A. Grimaldi, John A. Hartley -- Analysis of DNA-binding antibodies / Jeremy S. Lee, Laura J. Latimer, Jamshid Tanha -- Immunofluorescent staining of chromosomes with DNA-binding antibodies / Jeremy S. Lee, Laura J. Latimer, Gary D. Burkholder -- Optical absorbance and fluorescence techniques for measuring DNA-drug interactions / Terence C. Jenkins -- Evaluation of drug-nucleic acid interactions by thermal melting curves / W. David Wilson, Farial A. Tanious, Maria Fernandez-Saiz, C. Ted Rigl -- Electric dichroism / Dietmar Porschke -- Calorimetric techniques for studying drug-DNA interactions / Harry P. Hopkins -- Methods for the studies of drug dissociation from DNA / Fu-Ming Chen |
| Summary |
In Drug-DNA Interactions, expert researchers describe powerful molecular techniques designed to illuminate and explore the interaction of drugs and ligands with DNA, often perfected in their own laboratories, . These easily reproducible methods involve sequence recognition properties, as well as physical approaches to measuring both the strength of interaction and the mode of drug binding to DNA. Drug interactions are also examined from a cellular perspective and for their usefulness in the development of new therapeutic agents. Drug-DNA Interactions will be highly useful to all investigators needing the kind of detailed experimental and technical information often omitted from scientific papers. Those wishing to expand their repertoire of experimental techniques or to improve their current techniques will also find these powerful methods particularly helpful in elucidating the action of existing therapeutic agents, as well as in designing new anticancer drugs with improved action and reduced toxicity |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
|
English |
|
Print version record |
|
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| Subject |
DNA-drug interactions.
|
|
DNA probes.
|
|
DNA-binding proteins.
|
|
DNA -- drug effects
|
|
DNA Footprinting -- methods
|
|
DNA Probes
|
|
DNA-Binding Proteins
|
|
Polymerase Chain Reaction -- methods
|
|
DNA probes
|
|
DNA-binding proteins
|
|
DNA-drug interactions
|
|
Geneesmiddelen.
|
|
DNA.
|
|
Wetenschappelijke technieken.
|
| Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Fox, Keith R.
|
| ISBN |
9781592595747 |
|
159259574X |
|
1280837403 |
|
9781280837401 |
|
9786610837403 |
|
6610837406 |
|
