| Description |
1 online resource (x, 345 pages) : illustrations (chiefly color) |
| Contents |
Intro -- Foreword -- Preface -- Contents -- Part I: Current Status and Challenges of Plant Genome Editing Using CRISPR/Cas Technology -- Genome Engineering as a Tool for Enhancing Crop Traits: Lessons from CRISPR/Cas9 -- 1 Introduction -- 2 Zinc-Finger Nucleases -- 3 Transcription Activator-Like Effector Nucleases -- 4 CRISPR/Cas9 System -- 5 Mechanism of CRISPR/Cas9 System -- 6 Targeted Improvement of Crop Traits Using CRISPR/Cas9-Based Genome Editing in Cereals -- 6.1 Resistance Against Bacterial Disease -- 6.2 Resistance Against Fungal Disease -- 6.3 Resistance Against Viruses |
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6.4 Resistance and Tolerance Against Herbicides -- 6.5 Improved Quality and Yield -- 7 CRISPR/Cas9 Mediated Genome Editing in Horticultural Crops -- 7.1 Resistance Against Bacterial Disease -- 7.2 Resistance Against Fungal Disease -- 7.3 Resistance Against Viruses -- 7.4 Resistance and Tolerance Against Herbicide -- 7.5 Improved Quality and Yield -- 8 Conclusion -- References -- Vegetable Crop Improvement Through CRISPR Technology for Food Security -- 1 Introduction -- 2 Applications of Genome Editing in Improvement of Vegetable Crops -- 2.1 Qualitative Traits -- 2.1.1 Starch Content |
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2.1.2 Pigmentation -- 2.1.3 Saturated Fatty Acid Content -- 2.1.4 Improvement of Shelf-Life and Quality -- 2.1.5 Other Qualitative Traits -- 2.2 Abiotic Stress Tolerance -- 2.2.1 Drought and Extreme Temperature -- 2.2.2 Salinity and Mineral Deficiency -- 2.3 Pest and Disease Resistance -- 2.4 Biosafety and Legal Regulations -- 2.5 Conclusion -- References -- CRISPR/Cas9-Mediated Targeted Mutagenesis in Medicinal Plants -- 1 Introduction -- 2 CRISPR/Cas9 Mechanism -- 2.1 Cleavage Activity of Cas9 -- 3 CRISPR/Cas9 Vector System for Plants -- 3.1 sgRNA Expression Cassettes |
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3.2 Cas9 Expression Cassettes -- 4 CRISPR/dCas9 and Epigenome Editing in Plants -- 4.1 Nuclease-Dead Cas9 -- 4.2 sgRNA -- 4.3 Transcriptional Effectors -- 5 Analysis and Efficiency of Targeted Mutations -- 5.1 Reporter Genes -- 5.2 Single-Strand Conformation Polymorphism (SSCP) -- 5.3 High-Resolution Melting (HRM) -- 5.4 High-Throughput Sequencing (HTS or Deep Sequencing) -- 5.5 Sanger Sequencing -- 6 Medicinal Plants Modified Using CRISPR/Cas9 -- 6.1 Salvia militorrhiza -- 6.2 Dendrobium officinale -- 6.3 Dioscorea zingiberensis -- 7 Applications of Genome Editing in Medicinal Plants |
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8 Conclusion -- References -- Genome Editing: A Review of the Challenges and Approaches -- 1 Introduction -- 2 Mechanisms of Repairing Double-Stranded Breaks -- 2.1 Non-Homologous End Joining (NHEJ) -- 2.2 Genome editing and Homologous Recombination -- 2.2.1 History of Genome Editing -- 2.2.2 Homologous Recombination in E. coli -- 2.2.3 HR in Saccharomyces cerevisiae -- 2.2.4 HR in Higher Organisms -- 3 Different Genome Editing Techniques -- 3.1 Meganucleases (MNs) -- 3.2 Zinc Finger Nucleases (ZFNs) -- 3.3 Transcription Activator-Like Effector Nucleases (TALENs) |
| Summary |
Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms. Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement. Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com |
| Notes |
Includes index |
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Online resource; title from PDF title page (SpringerLink, viewed November 22, 2022) |
| Subject |
Transgenic plants.
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Crops -- Genetic engineering.
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Crops -- Genetic engineering
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Transgenic plants
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| Form |
Electronic book
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| Author |
Wani, Shabir Hussain, editor.
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Hensel, Goetz, editor
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| ISBN |
9783031080722 |
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3031080726 |
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