Title DNA topoisomerase protocols. Volume 95, Part 2, Enzymology and drugs / edited by Neil Osheroff and Mary-Ann Bjornsti

Published Totowa, N.J. : Humana Press, ©2001

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Description 1 online resource (1 volume) : illustrations Series Methods in molecular biology ; v. 95, pt. 2 Methods in molecular biology (Clifton, N.J.) ; volume 95, pt. 2 Contents Assaying DNA topoisomerase i relaxation activity / L. Stewart and J.J. Champoux -- DNA topoisomerase ii-catalyzed DNA decatenation / A. Haldane and D.M. Sullivan -- Plasmid DNA supercoiling by DNA gyrase / P.J. Sayer [and others] -- Analyzing reverse gyrase activity / M. Duguet [and others] -- Topoisomerase ii-catalyzed atp hydrolysis as monitored by thin-layer chromatography / P.S. Kingma, J.M. Fortune and N. Osheroff -- Use of a real-time, coupled assay to measure the atpase activity of DNA topoisomerase ii / J.E. Lindsley -- Analysis of topoisomerase-DNA interactions by electrophoretic mobility shift assay / S. Shuman -- Filter binding assays for topoisomerase-DNA complexes / J. Roca -- DNA topoisomerase i-mediated nicking of circular duplex DNA / J.J. Champoux -- Analysis of cleavage complexes using reactive inhibitor derivatives / K.N. Kreuzer, C.H. Freudenreich and Y. Pommier -- Uncoupling of topoisomerase-mediated DNA cleavage and religation / A.H. Andersen, K. Christiansen and O. Westergaard -- Synthesis and use of DNA containing a 5'-bridging phosphorothioate as a suicide substrate for type i DNA topoisomerases / A.B. Burgin -- Isolation of covalent enzyme-DNA complexes / T.C. Rowe, D. Grabowski and R. Ganapathi -- Ice bioassay. Isolating in vivo complexes of enzyme to DNA / D. Subramanian, C.S. Furbee and M.T. Muller -- DNA-unwinding test using eukaryotic DNA topoisomerase i / L.L. Shen -- Drug-DNA interactions / D.E. Graves -- Quinolone interactions with DNA and DNA gyrase / L.L. Shen -- Bactericidal assays for fluoroquinolones / T.D. Gootz -- Drug toxicity in e. Coli cells expressing human topoisomerase i / S.T. Taylor and R. Menzel -- Drug-induced cytotoxicity in tissue culture / C.E. Herzog and L.A. Zwelling -- Analysis of drug uptake-flow cytometry / G. Zoorob and T. Burke -- Cell-cycle analysis of drug-treated cells / F. Traganos, G. Juan and Z. Darzynkiewicz -- Assaying drug-induced apoptosis / Z. Darzynkiewicz, G. Juan and F. Traganos -- Single-strand conformational polymorphism analysis of DNA topoisomerases i and ii / M.K. Danks -- Pcr-based cloning of DNA topoisomerase genes / W.M. Huang -- Topoisomerase ii-catalyzed relaxation and catenation of plasmid DNA / J.M. Fortune and N. Osheroff -- Topoisomerase ii-mediated cleavage of plasmid DNA / D.A. Burden, S.J. Froelich-Ammon and N. Osheroff -- Drug-induced stabilization of covalent DNA topoisomerase i-DNA intermediates. DNA cleavage assays / P. Fiorani [and others] -- Studying DNA topoisomerase i-targeted drugs in the yeast. Saccharomyces cerevisiae / M.H. Woo, J.R. Vance and M.A. Bjornsti -- Yeast systems for demonstrating the targets of anti-topoisomerase ii agents / J.L. Nitiss and K.C. Nitiss Summary Volume II: Enzymology and Drugs contains detailed methodologies for the analysis of enzyme activities, including the relaxation of supercoiied DNA, DNA unknotting and decatenation, ATP hydrolysis, and the formation of drugstabilized, covalent-enzymc DNA intermediates. Additional chapters describe methods for analyzing topoisomerase-iargeted drug action. A variety of experimental ystems are presented to assess the cytotoxic action opoisomerase-directed agents, as well as methods for selecting and analyzing drug-resistant DNA topoisomerase mutants Analysis dna isomerasen isomerases geneesmiddelen drugs biologische technieken biological techniques protocollen protocols Nucleic Acids Bibliography Includes bibliographical references and index Notes Print version record Subject DNA -- Structure -- Laboratory manuals DNA -- Conformation -- Laboratory manuals DNA topoisomerases. DNA topoisomerase I. DNA Topoisomerases, Type I DNA Topoisomerases SCIENCE -- Life Sciences -- Genetics & Genomics. DNA topoisomerase I DNA -- Conformation DNA -- Structure DNA topoisomerases Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Osheroff, Neil. Bjornsti, Mary-Ann. ISBN 9781592590575 1592590578

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