| Description |
1 online resource (xvi, 434 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; volume 1799 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1799. 1064-3745
|
| Contents |
Fungal protease model to interrogate allergic lung immunity / J. Morgan Knight, Evan Li, Hui-Ying Tung, Cameron Landers, Jake Wheeler, Farrah Kheradmand, and David B. Corry -- Use of the Litomosoides sigmodontis infection model of filariasis to study type 2 immunity / A. Fulton, S. A. Babayan, and M. D. Taylor -- Production of Hymenolepis diminuta in the laboratory : an old research tool with new clinical applications / Min Zhang, Amanda J. Mathew, and William Parker -- Mouse model of peanut allergy induced by sensitization through the gastrointestinal tract / Kelly Orgel and Michael Kulis -- Induction and characterization of the allergic eye disease mouse model / Nancy J. Reyes, Rose Mathew, and Daniel R. Saban -- Isolation and purification of epithelial and endothelial cells from mouse lung / Hideki Nakano, Keiko Nakano, and Donald N. Cook -- In vitro and in vivo IgE-/antigen-mediated mast cell activation / Hae Woong Choi and Soman N. Abraham -- Use of human and mouse mast cell and basophil cultures to assess type 2 inflammation / Heather L. Caslin, Marcela T. Taruselli, Anuya Paranjape, Kasalina Kiwanuka, Tamara Haque, Alena P. Chumanevich, Carole A. Oskeritzian, and John J. Ryan -- Isolation and identification of group 2 innate lymphoid cells in settings of type 2 inflammation / Jesse Charles Nussbaum and Jorge Felipe Ortiz-Carpena -- Determination of the fate and function of innate lymphoid cells following adoptive transfer of innate lymphoid cell precursors / Timothy E. O'Sullivan and Joseph C. Sun -- Characterization of thymic development of natural killer T cell subsets by multiparameter flow cytometry / Kathryn D. Tuttle and Laurent Gapin -- Characterization of mouse ?? T cell subsets in the setting of type-2 immunity / Wanjiang Zeng, Rebecca L. O'Brien, Willi K. Born, and Yafei Huang -- Identification of allergen-derived T cell epitopes / Véronique Schulten and Alessandro Sette -- Generation of allergen-specific tetramers for a murine model of airway inflammation / James J. Moon and Marion Pepper -- Generation and use of allergen-specific TCR transgenic animals / Manon Vanheerswynghels, Wendy Toussaint, Martijn Schuijs, Leen Vanhoutte, Nigel Killeen, Hamida Hammad, and Bart N. Lambrecht -- Using cytokine reporter mice to visualize type-2 immunity in vivo / Mark Dell'Aringa and R. Lee Reinhardt -- Live imaging of il-4-expressing T follicular helper cells in explanted lymph nodes / Mark Dell'Aringa, R. Lee Reinhardt, Rachel S. Friedman, and Jordan Jacobelli -- Imaging precision-cut lung slices to visualize leukocyte localization and trafficking / Miranda R. Lyons-Cohen, Hideki Nakano, Seddon Y. Thomas, and Donald N. Cook -- Study of IgE-producing B cells using the verigem fluorescent reporter mouse / Zhiyong Yang, James B. Jung, and Christopher D. C. Allen -- Chromatin preparation from murine eosinophils for genome-wide analyses / Carine Bouffi, Artem Barski, and Patricia C. Fulkerson -- Sensitive and integrated approach to profile messenger RNA from samples with low cell numbers / Sandy Lisette Rosales, Shu Liang, Isaac Engel, Benjamin Joachim Schmiedel, Mitchell Kronenberg, Pandurangan Vijayanand, and Grégory Seumois -- Integrated and semiautomated microscaled approach to profile cis-regulatory elements by histone modification ChIP-Seq for large-ccale epigenetic studies / Diana Youhanna Jankeel, Justin Cayford, Benjamin Joachim Schmiedel, Pandurangan Vijayanand, and Grégory Seumois -- Library preparation for ATAC-sequencing of mouse CD4+ T cells isolated from the lung and lymph nodes after helminth infection / Laura D. Harmacek, Preeyam Patel, Rachel Woolaver, R. Lee Reinhardt, and Brian P. O'Connor -- Identification of functionally relevant microRNAs in the regulation of allergic inflammation / Marlys S. Fassett, Heather H. Pua, Laura J. Simpson, David F. Steiner, and K. Mark Ansel -- Use of biodegradable nanoparticles for tolerogenic therapy of allergic inflammation / Charles B. Smarr and Stephen D. Miller -- Assessing the mouse intestinal microbiota in settings of type-2 immune responses / Mei San Tang, Rowann Bowcutt, and P'ng Loke -- Use of CRISPR-Cas9 technology to reveal important aspects of human airway biology / Azzeddine Dakhama and Hong Wei Chu -- Consistent method to identify and isolate mononuclear phagocytes from human lung and lymph nodes / Sophie L. Gibbings and Claudia V. Jakubzick -- Organoid cultures for assessing intestinal epithelial differentiation and function in response to type-2 inflammation / Bailey Zwarycz, Adam D. Gracz, and Scott T. Magness -- Utilization of air-liquid interface cultures as an in vitro model to assess primary airway epithelial cell responses to the type 2 cytokine interleukin-13 / Jamie L. Everman, Cydney Rios, and Max A. Seibold |
| Summary |
Annotation This book provides researchers the opportunity to investigate type-2-associated diseases in their laboratories. Beginning with chapters describing various models of type-2 immunity, the volume then continues by detailing cellular protocols designed to identify, characterize, and assess the function of key adaptive and innate immune cells involved in type-2 inflammation; approaches to isolate and evaluate specific cellular subsets at the genetic, epigenetic, and molecular level; protocols to assess type-2 immunity and its relationship to organismal and metabolic systems (ex. Microbiome). This book concludes with a section that explores the use of primary human cells in evaluating relevance to the clinic. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Vital and authoritative, Type 2 Immunity: Methods and Protocols aims to provide a broad network of methods that can be used to develop a hypothesis and investigate its potential from bench to beside |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocol, viewed June 29, 2018) |
| Subject |
Immunity -- Laboratory manuals
|
|
Immunity.
|
|
Immunity
|
|
Immunology.
|
|
Medical -- Immunology.
|
|
Immunity
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Reinhardt, R. Lee, editor
|
| ISBN |
9781493978960 |
|
1493978969 |
|
1493978950 |
|
9781493978953 |
|
