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Title Metabolic signaling : methods and protocols / edited by Sarah-Maria Fendt, Sophia Y. Lunt
Published New York, N.Y. : Humana Press : Springer, [2018]
©2019

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Description 1 online resource (xii, 294 pages) : illustrations (some color)
Series Methods in Molecular Biology ; v. 1862
Methods in molecular biology (Clifton, N.J.) ; v. 1862. 1064-3745
Contents Protocol to compare methods for untargeted metabolomics / Lingjue Wang, Fuad J. Naser, Jonathan L. Spalding, and Gary J. Patti -- High-throughput measure of bioactive lipids using non-targeted mass spectrometry / Kim A. Lagerborg, Jeramie D. Watrous, Susan Cheng, and Mohit Jain-- Measuring the nutrient metabolism of adherent cells in culture / Martin P. Ogrodzinski, Shao Thing Teoh, Lei Yu, Deanna Broadwater, Elliot Ensink, and Sophia Y. Lunt -- ¹³C tracer analysis and metabolomics in 3D cultured cancer cells / Marit van Gorsel, Ilaria Elia, and Sarah-Maria Fendt -- Measuring in vivo tissue metabolism using 13C glucose infusions in mice / Dorien Broekaert and Sarah-Maria Fendt -- Measuring human lipid metabolism using deuterium labeling : in vivo and in vitro protocols / Katherine E. Pinnick, Pippa J. Gunn, and Leanne Hodson -- Measuring rates of ATP synthesis / Matthew J. Bird, Silvia Radenkovic, Pieter Vermeersch, and David Cassiman -- Direct estimation of metabolic flux by heavy isotope labeling simultaneous with pathway inhibition : metabolic flux inhibition assay / Tong Zhang, Christiaan F. Labuschagne, Karen H. Vousden, and Oliver D.K. Maddocks -- Measuring glycolytic and mitochondrial fluxes in endothelial cells using radioactive tracers / Koen Veys, Abdiel Alvarado-Diaz, and Katrien De Bock -- Determining compartment-specific metabolic fluxes / Yannic Nonnenmacher, Roberta Palorini, and Karsten Hiller -- Determining the impact of metabolic nutrients on autophagy / Jessica D. Guillaume, Stephanie L. Celano, Katie R. Martin, and Jeffrey P. MacKeigan -- Measuring the activation of cell death pathways upon inhibition of metabolism / Franziska Puschel and Cristina Munoz-Pinedo -- Determining macrophage polarization upon metabolic perturbation / Pu-Ste Liu and Ping-Chih Ho -- Assessing the impact of the nutrient microenvironment on the metabolism of effector CD8+ T cells / Juan Fernandez Garcia and Sarah-Maria Fendt -- Development of patient-derived tumor xenograft models / Daniela Annibali, Eleonora Leucci, Els Hermans, and Frederic Amant -- Imaging glioma progression by intravital microscopy / Fabio Stanchi, Ken Matsumoto, and Holger Gerhardt -- Lipectomizing mice for applications in metabolism / Debrup Chakraborty and Jamie J. Bernard -- Quantitative multiplex immunoassay for profiling bone turnover biomarkers in human bone tissue culture supernatants / Wen-Rong Lie, Derek F. Amanatullah, and Bonnie L. King -- Determining the intracellular organization of organelles / Bruno Latge and Kristine Schauer -- Fundamentals of constructing and interpreting heat maps / Nathaniel M. Vacanti
Notes Online resource; title from PDF title page (SpringerLink, viewed October 12, 2018)
Subject Metabolism -- Laboratory manuals
Metabolism.
Metabolism
metabolism (biological concept)
Cellular biology (cytology)
Science -- Life Sciences -- Biology -- Molecular Biology.
Metabolism
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Fendt, Sarah-Maria, editor
Lunt, Sophia Y., editor
ISBN 9781493987696
1493987690