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Title Plant metabolism : methods and protocols / edited by Ganesh Sriram, Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD, USA
Published New York : Humana Press, [2014]
©2014

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Description 1 online resource (x, 318 pages) : illustrations (some color)
Series Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1083
Methods in molecular biology (Clifton, N.J.) ; v. 1083. 1064-3745
Contents MeRy-B, a metabolomic database and knowledge base for exploring plant primary metabolism / Catherine Deborde and Daniel Jacob -- Targeted deuteration of polyphenolics for their qualitative and quantitative metabolomic analysis in plant-derived extracts / Mikel R. Roe, Jerry D. Cohen, and Adrian D. Hegeman -- Relative quantitation in single-cell metabolomics by laser ablation electrospray mass spectrometry / Bindesh Shrestha and Akos Vertes -- Quantification of plant volatiles / Anthony V. Qualley and Natalia Dudareva -- Quantitative imaging approaches for small-molecule measurements using FRET sensors in plants / Sakiko Okumoto -- Isotopomer measurement techniques in metabolic flux analysis I : nuclear magnetic resonance / Quyen X. Truong, Jong Moon Yoon, and Jacqueline V. Shanks -- Isotopomer measurement techniques in metabolic flux analysis II : mass spectrometry / Jamey D. Young, Douglas K. Allen, and John A. Morgan -- Mathematical modeling of isotope labeling experiments for metabolic flux analysis / Shilpa Nargund and Ganesh Sriram -- Optimal design of isotope labeling experiments / Hong Yang, Dominic E. Mandy, and Igor G.L. Libourel -- Putting the plant metabolic network pathway databases to work : going offline to gain new capabilities / Kate Dreher -- Elucidation of metabolic pathways from enzyme classification data / Andrew G. McDonald and Keith F. Tipton -- Deducing intracellular distributions of metabolic pathways from genomic data / Ansgar Gruber and Peter G. Kroth -- Genome-scale models of plant metabolism / Margaret Simons, Ashish Misra, and Ganesh Sriram -- Elementary flux modes, flux balance analysis, and their application to plant metabolim / Katrin Lotz [and four others] -- Systems approaches to unraveling plant metabolism : identifying biosynthetic genes of secondary metabolic pathways / Martin J. Spiering [and three others] -- Applications of kinetic modeling to plant metabolism / Johann M. Rohwer -- Kinetic modeling of plant metabolism and its predictive power : peppermint essential oil biosynthesis as an example / Bernd Markus Lange and Rigoberto Rios-Estepa
Summary In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, reagents, or software, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Metabolism: Methods and Protocols seeks to benefit scientists ranging from plant biology, metabolic engineering, and biotechnology
Bibliography Includes bibliographical references and index
Notes English
Online resource; title from PDF title page (SpringerLink, viewed November 18, 2013)
Subject Plants -- Metabolism -- Laboratory manuals
Metabolism.
Metabolism
Eukaryota
Publication Formats
Organisms
Phenomena and Processes
Publication Characteristics
Laboratory Manuals
Plants
metabolism (biological concept)
vegetation.
Metabolism
Plants -- Metabolism
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Sriram, Ganesh, editor
ISBN 9781627036610
162703661X
1627036601
9781627036603