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Book Cover
E-book

Title Plant epigenetics and epigenomics : methods and protocols / edited by Charles M. Spillane, Peter C. McKeown
Published New York : Humana Press, [2014]
©2014

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Description 1 online resource (x, 245 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; 1112
Methods in molecular biology (Clifton, N.J.) ; v. 1112. 1064-3745
Contents Landscaping Plant Epigenetics -- The Gene Balance Hypothesis: Dosage Effects in Plants -- High-throughput RNA-Seq for Allelic or Locus-specific Expression Analysis in Arabidopsis Related Species, Hybrids, and Allotetraploids -- Inference of Allele-specific Expression from RNA-seq Data -- Screening for Imprinted Genes using High Resolution Melting Analysis of PCR Amplicons -- Analysis of Genomic Imprinting by Quantitative Allele-specific Expression by Pyrosequencing -- Endosperm-specific Chromatin Profiling by Fluorescence-activated Nuclei Sorting and Chip-on-chip -- Imaging Sexual Reproduction in Arabidopsis using Fluorescent Markers -- Genome-wide Analysis of DNA Methylation in Arabidopsis using MeDIP-chip -- Methylation-Sensitive Amplified Polymorphism (MSAP) Marker to Investigate Drought-stress Response in Montepulciano and Sangiovese Grape Cultivars -- Detecting Histone Modifications in Plants -- Quantitatively Profiling Genome-wide Patterns of Histone Modifications in Arabidopsis thaliana using ChIP-seq -- Analysis of Retrotransposon Activity in Plants -- Detecting Epigenetic Effects of Transposable Elements in Plants -- Detection and Investigation of Transitive Gene Silencing Effects in Plants
Summary Many fundamental discoveries concerning epigenetics and the elucidation of mechanisms of epigenetic regulation have developed from studies performed in plants. In Plant Epigenetics and Epigenomics: Methods and Protocols, leading scientists in the epigenetics field describe comprehensive techniques that have been developed to understand the plant epigenetic landscape. These include recently developed methods and techniques for analysis of epigenetically regulated traits, such as flowering time, transposon activation, genomic imprinting and genome dosage effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoidance of known pitfalls. Authoritative and practical, Plant Epigenetics and Epigenomics: Methods and Protocols seeks to aid scientists in the further study of plant epigenetic phenomena using advanced contemporary methods
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Plant genetics.
Epigenetics.
Plant genomes.
Biology.
Genetic regulation.
Life sciences.
Physical sciences.
Botany.
Plants.
Biology
Publication Formats
Eukaryota
Gene Expression Regulation
Organisms
Biological Science Disciplines
Genetic Phenomena
Publication Characteristics
Natural Science Disciplines
Disciplines and Occupations
Phenomena and Processes
Plants
Epigenesis, Genetic
Laboratory Manuals
Genetics
biology.
biological sciences.
physical sciences.
vegetation.
Plants
Physical sciences
Life sciences
Genetic regulation
Botany
Biology
Epigenetics
Plant genetics
Plant genomes
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Spillane, Charlie, editor
McKeown, Peter C., editor
ISBN 9781627037730
162703773X
1627037721
9781627037723