Title Insect molecular genetics : an introduction to principles and applications / Marjorie A. Hoy

Edition Third edition Published Amsterdam : Academic Press, [2013] ©2013

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Description 1 online resource (xxvii, 808 pages) : illustrations (some color) Contents 880-01 Note continued: 9.3.P Elements and Hybrid Dysgenesis -- 9.4.P-Element Structure Varies -- 9.5. Transposition Method of P Elements -- 9.6. Origin of P Elements in D. melanogaster -- 9.7.P Vectors and Germ-Line Transformation -- 9.7.1. Protocols -- 9.7.2. Characterizing Transformants -- 9.8. Using P-Element Vectors -- 9.8.1. Transposon Tagging -- 9.8.2. Expressing Exogenous Genes -- 9.8.3. Evaluating Position Effects -- 9.8.4. Targeted Gene Transfer -- 9.9. Transformation of Other Insects with P Vectors -- 9.10. Evolution of Resistance to P Elements -- 9.11. Using P to Drive Genes into Populations -- 9.12. Relationship of P to Other Transposable Elements (TEs) -- 9.13. Other TEs Can Transform D. melanogaster -- 9.14. Improved Transformation Tools for Drosophila -- 9.15. TE Vectors to Transform Insects other than Drosophila -- 9.15.1.piggyBac -- 9.15.2. Hermes and Herves -- 9.15.3. Minos -- 9.15.4.mariner -- 9.15.5.hobo -- 9.16. Cross Mobilization of TE Vectors -- 9.17. Conversion of Inactive TE Vectors to Activity -- 9.18. Suppression of Transgene Expression -- 9.19. Other Transformation Methods -- 9.19.1. JcDNV Gene Vectors for Somatic Transformations v -- 9.19.2. RNAi for Drosophila -- 9.19.3. Zinc-Finger Nucleases (ZFNs) -- 9.19.4. Transcription Activator-Like Effector Nucleases (TALENs) -- 9.19.5. Meganucleases (or Homing Endonucleases) -- 9.19.6. Cell-Penetrating Peptides -- 9.19.7. Nanotechnology Approaches -- 9.20. Conclusions -- General References -- References Cited -- pt. III APPLICATIONS IN ENTOMOLOGY -- ch. 10 Sex Determination in Insects -- 10.1. Overview -- 10.2. Introduction -- 10.3. Costs and Benefits of Sexual Reproduction -- 10.3.1. Sexual Reproduction Has Costs -- 10.3.2. Advantages of Sex Must Be Large -- 10.3.3. Origin of Sex -- 10.4. Sex Determination Involves Soma and Germ-Line Tissues -- 10.5. Sex Determination in Drosophila melanogaster -- 10.5.1. Dosage Compensation of X Chromosomes -- 10.5.2. Somatic-Sex Determination -- 10.5.3. Germ-Line Determination -- 10.6. Are Sex-Determination Mechanisms Diverse? -- 10.6.1. Intraspecific Variability -- 10.6.2. Environmental Effects -- 10.6.3. Postzygotic Sex Determination -- 10.7.A Single Model? -- 10.8. Meiotic Drive Can Distort Sex Ratios -- 10.8.1. Segregation Distorter (SD) -- 10.8.2. Distorter in Mosquitoes -- 10.8.3. Female-Biased Sex Ratios in Stalk-Eyed Flies -- 10.8.4. Meiotic Drive as a Pest-Management Tool? -- 10.9. Hybrid Sterility -- 10.10. Medea in Tribolium -- 10.11. Cytoplasmic Agents Distort Normal Sex Ratios -- 10.11.1. Spiroplasma Strains -- 10.11.2.L-Form Bacteria -- 10.11.3. Rickettsia -- 10.11.4. Wolbachia -- 10.11.5. Cardinium -- 10.12. Paternal Sex-Ratio Chromosomes and Cytoplasmic Incompatibility in Nasonia -- 10.13. Male Killing in the Coccinellidae -- 10.14. Sex and the Sorted Insects -- 10.14.1. Genetic Control -- 10.14.2. Genetic Improvement of Parasitoids -- 10.15. Conclusion -- References Cited -- ch. 11 Molecular Genetics of Insect Behavior -- 11.1. Overview -- 11.2. Introduction -- 11.3. The Insect Nervous System -- 11.4. Traditional Genetic Analyses of Behavior -- 11.4.1. Crossing Experiments -- 11.4.2. Selection Experiments -- 11.4.3. Some Polygenically Determined Behaviors -- 11.5. Molecular-Genetic Analyses of Insect Behavior -- 11.5.1. The Photoperiodic Clock -- 11.5.2. Learning in Drosophila -- 11.5.3. Functional Genomics of Odor Behavior in Drosophila -- 11.5.4. Behavior of Apis mellifera -- 11.5.5. Pheromones in Insects -- 11.5.6. Neurobiochemistry of Drosophila -- 11.5.7. Divergent Functions of Est-6 and Est-5 in Two Drosophila Species: A Cautionary Tale of Homologs -- 11.5.8. Courtship Behavior in Drosophila -- 11.5.9. Speciation Genes in Drosophila and Other Insects -- 11.5.10. Personality in Insects: Tribolium confusum, Apis mellifera, Acyrthosiphon pisum, and Pyrrhocoris apterus -- 11.6. Symbionts and Insect Behavior -- 11.7. Human Neurodegenerative Diseases and Addictions in Drosophila -- 11.8. High-Throughput Ethomics -- 11.9. Systems Genetics of Complex Traits in Drosophila -- 11.10. Social Behavior in Bees and Ants -- 11.11. Conclusions -- References Cited -- ch. 12 Molecular Systematics and the Evolution of Arthropods -- 12.1. Overview -- 12.2. Introduction -- 12.3. Controversies in Molecular Systematics and Evolution -- 12.3.1. Molecular versus Morphological Traits -- 12.3.2. The Molecular Clock -- 12.3.3. The Neutral (or Nearly Neutral) Theory of Evolution -- 12.3.4. Homology and Similarity -- 12.4. Molecular Methods for Molecular Systematics and Evolution -- 12.4.1. Protein Electrophoresis -- 12.4.2. Molecular Cytology -- 12.4.3. Restriction Fragment Length Polymorphism (RFLP) Analysis -- 12.4.4. DNA and Genome Sequencing -- 12.4.5. Fragment Analyses of Genomic DNA -- 12.5. Targets of DNA Analysis -- 12.5.1. Mitochondria -- 12.5.2. Ribosomal RNA -- 12.5.3. Satellite DNA -- 12.5.4. Introns -- 12.5.5. Nuclear Protein-Coding Genes -- 12.5.6. Rare Genomic Changes -- 12.5.7. MicroRNAs -- 12.6. Steps in Phylogenetic Analysis of DNA Sequence Data -- 12.6.1. Gene Trees or Species Trees -- 12.6.2. Rooted or Unrooted Trees -- 12.6.3. Tree Types -- 12.6.4. Project Goals and Appropriate DNA Sequences -- 12.6.5. Sequence Comparisons with BLAST -- 12.6.6. Aligning Sequences -- 12.6.7. Constructing Phylogenies -- 12.6.8. Artifacts -- 12.6.9. Software Packages -- 12.7. The Universal Tree of Life -- 12.7.1. Two Domains -- 12.7.2. Three Domains -- 12.7.3. Origin of Eukaryota -- 12.8. The Fossil Record of Arthropods -- 12.9. Molecular Analyses of Arthropod Phylogeny -- 12.9.1. Evolution of the Ecdysozoa -- 12.9.2. Relationships among the Arthropoda -- 12.9.3. The Phylogeny of the Holometabola -- 12.9.4. Congruence Between Morphology- and Molecular-Based Trees -- 12.9.5. Genomes and Arthropod Phylogenies -- 12.10. Molecular Evolution and Speciation -- 12.10.1. Species Concepts -- 12.10.2. How Many Genes are Involved in Speciation? -- 12.10.3. Detecting Cryptic Species -- 12.11. Some Conclusions -- Relevant Journals -- References Cited -- ch. 13 Insect Population Ecology and Molecular Genetics -- 13.1. Overview -- 13.2. Introduction -- 13.3. What is Molecular Ecology? -- 13.4. Collecting Arthropods in the Field for Analysis -- 13.5. Molecular Ecological Methods -- 13.5.1. Allele-Specific PCR -- 13.5.2. Allozymes (Protein Electrophoresis) -- 13.5.3. Amplified Fragment Length Polymorphisms (AFLP-PCR) -- 13.5.4. Double-Strand Conformation Polymorphism (DSCP) -- 13.5.5. Heteroduplex Analysis (HDA) -- 13.5.6. Microarrays -- 13.5.7. Microsatellites -- 13.5.8. RFLP Analysis -- 13.5.9. PCR-RFLP -- 13.5.10. RAPD-PCR -- 13.5.11. Sequencing -- 13.5.12. Single Nucleotide Polymorphism (SNP) Markers -- 13.6. Analysis of Molecular Data -- 13.6.1. Allozymes -- 13.6.2. Microsatellites -- 13.6.3. RAPD-PCR -- 13.6.4. RFLPs -- 13.6.5. Sequencing -- 13.7. Case Studies in Molecular Ecology and Population Biology -- 13.7.1. Genetic Variability in the Fall Army worm: Incipient Species or Multiple Species? -- 13.7.2. Analyses of Natural Enemies -- 13.7.3. Population Isolation and Introgression in Periodical Cicadas -- 13.7.4. Eradicating Medflies in California? -- 13.7.5. Plant Defenses to Insect Herbivory -- 13.7.6. Origins of Insect Populations -- 13.8. Applied Pest Management -- 13.8.1. Monitoring Biotypes, Species, and Cryptic Species -- 13.8.2. Monitoring Vectors of Disease -- 13.8.3. Pesticide Resistances and Pest Management -- 13.8.4. Monitoring Pest-Population Biology -- 13.8.5. The "So What?" Test -- Relevant Journals -- References Cited -- ch. 14 Genetic Modification of Pest and Beneficial Insects for Pest-Management Programs -- 14.1. Overview -- 14.2. Introduction -- 14.3. Why Genetically Modify Insects? -- 14.3.1. Beneficial Insects -- 14.3.2. Pest Insects -- 14.4. Why Use Molecular-Genetic Methods? -- 14.5. What Genetic Modification Methods are Available? -- 14.5.1. Transposable-Element (TE) Vectors and Transgenesis -- 14.5.2. Paratransgenesis (Genetic Modification of Symbionts) -- 14.5.3. Viral Vectors -- 14.5.4. Transfer of Wolbachia from Another Arthropod -- 14.5.5. Site-Specific Modifications -- 14.5.6. No Vectors -- 14.5.7. RNAi to Control Pests -- 14.6. Methods to Deliver Exogenous Nucleic Acids into Arthropod Tissues -- 14.7. What Genes are Available? -- 14.8. Why are Regulatory Signals Important? -- 14.9. How are Modified Arthropods Identified? -- 14.10. How to Deploy Genetically Modified Pest and Beneficial Arthropods -- 14.11. Potential Risks Associated with Releases of Genetically Modified Arthropods -- 14.11.1. Could Gene Silencing Reduce Program Effectiveness? -- 14.11.2. Relative Risks -- 14.11.3. General Risk Issues -- 14.11.4. Horizontal Transfer (HT) -- 14.12. Permanent Releases of Genetically Modified Arthropods into the Environment -- 14.12.1. Models to Predict? -- 14.13. Regulatory Issues: Releases of Genetically Modified Arthropods -- 14.14. Conclusions -- References Cited 880-01/(S 4.17. Evo-Devo and the Revolution in Developmental Studies -- References Cited -- pt. II MOLECULAR GENETIC TECHNIQUES -- ch. 5 Some Basic Tools: How to Cut, Paste, Copy, Measure, Visualize, and Clone DNA -- 5.1. Overview -- 5.2. Introduction to a Basic Molecular Biology Experiment -- 5.2.1. The Pros and Cons of Kits -- 5.2.2. A Simple Cloning Experiment -- 5.3. Extracting DNA from Insects -- 5.3.1. DNA Extraction Resulting in Loss of the Specimens -- 5.3.2. DNA Extraction That Does Not Require Destroying the Specimens -- 5.3.3. Assessing the Quality of Extracted DNA -- 5.4. Precipitating Nucleic Acids -- 5.5. Shearing DNA -- 5.6. Cutting DNA with Restriction Endonucleases -- 5.7. Joining DNA Molecules -- 5.8. Growth, Maintenance, and Storage of E. coli -- 5.9. Plasmids for Cloning in E. coli -- 5.10. Transforming E. coli with Plasmids -- 5.11. Purifying Plasmid DNA from E. coli -- 5.12. Electrophoresis in Agarose or Acrylamide Gels -- 5.13. Detecting, Viewing, and Photographing Nucleic Acids in Gels -- 5.14. Identifying Specific DNA by Southern Blot Analysis -- 5.15. Labeling DNA or RNA Probes -- 5.16. Removing DNA from Agarose Gels after Electrophoresis -- 5.17. Restriction-Site Mapping -- General References -- References Cited -- ch. 6 Some Additional Tools for the Molecular Biologist -- 6.1. Overview -- 6.2. Introduction -- 6.3. The Perfect Genomic Library -- 6.3.1. Lambda (λ) Phage as a Vector -- 6.3.2. Cloning with Cosmids -- 6.3.3. Cloning in the Filamentous Phage M13 -- 6.3.4. Phagemids -- 6.3.5. BACs -- 6.4. cDNA Cloning -- 6.5. Enzymes Used in Molecular Biology Experiments -- 6.6. Isolating a Specific Gene from a Library if Whole-Genome Sequencing is Not Done -- 6.7. Labeling Probes by a Variety of Methods -- 6.7.1. Synthesis of Uniformly Labeled DNA Probes by Random Primers -- 6.7.2. Synthesis of Probes by Primer Extension -- 6.7.3. End-Labeled Probes -- 6.7.4. Single-Stranded Probes -- 6.7.5. Synthetic Probes -- 6.8. Baculovirus Vectors Express Foreign Polypeptides in Insect Cells -- 6.9. Expression Microarray Analysis -- General References -- References Cited -- ch. 7 DNA Sequencing and the Evolution of the "-Omics" -- 7.1. Overview -- 7.2. Introduction -- 7.3. The Dideoxy or Chain-Termination (Sanger) Method -- 7.4. The Maxam and Gilbert Sequencing Method -- 7.5. Shotgun Strategies for Genomes -- 7.6. Sequencing DNA by the Polymerase Chain Reaction (PCR) -- 7.7. Automated Sanger Sequencers -- 7.7.1. Decreasing Costs of Sanger Sequencing -- 7.8. Analyzing DNA Sequence Data -- 7.9. DNA-Sequence Data Banks -- 7.10. A Brief History of the Drosophila Genome Project -- 7.10.1. The Original Drosophila Genome Project -- 7.10.2. The Actual Drosophila Genome Project -- 7.10.3. Drosophila Genome Analysis -- 7.10.4. Surprises in the Drosophila Genome -- 7.11. Next-Generation Sequencing Methods and Beyond -- 7.11.1. Next-Generation (NextGen or Second-Generation) Sequencing -- 7.11.2. Third-Generation Sequencing -- 7.12. Bioinformatics -- 7.12.1. Gene Ontology -- 7.13. Genome Analyses of Other Arthropods -- 7.13.1. Interesting Findings from Completed Genomes -- 7.13.2. What Do You Need to Do to Sequence Your Favorite Insect's Genome-- 7.14. Transposable Elements (TEs) as Agents of Genome Evolution -- 7.15. Transcriptomics -- 7.15.1. Tiling Microarrays -- 7.16. Metagenomics -- 7.17. Proteomics: Another "-Omic" -- 7.18. Functional Genomics -- 7.19. Structural Genomics---Another New Horizon-- 7.20. Comparative Genomics -- 7.21. Interactomes or Reactomes -- 7.22. The Post-Genomic Era: Systems Genetics -- General References -- References Cited -- ch. 8 DNA Amplification by the Polymerase Chain Reaction: Molecular Biology Made Accessible -- 8.1. Overview -- 8.2. Introduction -- 8.3. The Basic Polymerase Chain Reaction (PCR) -- 8.3.1. The First Few Cycles are Critical -- 8.3.2. PCR Power -- 8.3.3. Standard PCR Protocols -- 8.3.4. DNA Polymerases -- 8.3.5. Other Thermostable DNA Polymerases -- 8.3.6. Primers are Primary -- 8.3.7. Storing Insects for the PCR -- 8.3.8. Preparing DNA Samples -- 8.3.9. PCR Automation -- 8.3.10. Specificity of the PCR -- 8.3.11. Detecting Primer Artifacts -- 8.3.12. How Many Cycles Does a PCR Need-- 8.3.13. Reducing the Evils of Contamination -- 8.4. Some Modifications of the PCR -- 8.4.1. AFLP for DNA Fingerprinting -- 8.4.2. Anchored PCR -- 8.4.3. Arbitrary Primers -- 8.4.4. Asymmetric PCR -- 8.4.5. Degenerate Primers -- 8.4.6. Hot-Start PCR -- 8.4.7. Inverse PCR -- 8.4.8. Long PCR or High-Fidelity PCR -- 8.4.9. Multiplex PCR -- 8.4.10. Nested PCR -- 8.4.11. PCR-RFLP -- 8.4.12. Quantitative PCR -- 8.4.13. Random Primers Summary This book summarizes and synthesizes two rather disparate disciplines-entomology and molecular genetics. It provides an introduction to the techniques and literature of molecular genetics; defines terminology; and reviews concepts, principles, and applications of these powerful tools Bibliography Includes bibliographical references and index Notes Print version record Subject Insects -- Molecular genetics Arthropoda. Insects. Molecular structure. Arthropods Chemical Phenomena Phenomena and Processes Biochemical Phenomena Invertebrates Animals Eukaryota Organisms Insecta Molecular Structure Genetic Phenomena molecular structure. Insecta (class) SCIENCE -- Life Sciences -- Molecular Biology. Molecular structure Insects Arthropoda Insects -- Molecular genetics Form Electronic book ISBN 9780240821313 0240821319

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