Title Plant lipid signaling protocols / edited by Teun Munnik, Ingo Heilmann

Published New York : Humana Press, ©2013

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Description 1 online resource (xi, 305 pages) : illustrations Series Methods in molecular biology, 1340-6029 ; 1009 Methods in molecular biology (Clifton, N.J.) ; v. 1009. 1064-3745 Contents Analyzing plant signaling phospholipids through 32Pi-labeling and TLC / Teun Munnik and Xavier Zarza -- Analysis of D3-,4-,5-phosphorylated phosphoinositides using HPLC / Teun Munnik -- Mass measurement of polyphosphoinositides by thin-layer and gas chromatography / Mareike Heilmann and Ingo Heilmann -- Measurement of inositol (1,4,5) trisphosphate in plant tissues by a competitive receptor binding assay / Ingo Heilmann and Imara Y. Perera -- Quantification of diacylglycerol by mass spectrometry / Katharina vom Dorp, Isabel Dombrink, and Peter Dörmann -- Distinguishing phosphatidic acid pools from de novo synthesis, PLD, and DGK / Steven A. Arisz and Teun Munnik -- Use of phospholipase A2 for the production of lysophospholipids / Steven A. Arisz and Teun Munnik -- Analysis and quantification of plant membrane lipids by thin-layer chromatography and gas chromatography / Vera Wewer, Peter Dörmann, and Georg Hölzl -- Lipidomic analysis of plant membrane lipids by direct infusion tandem mass spectrometry / Sunitha Shiva [and others] -- Detection and quantification of plant sphingolipids by LC-MS / Jonathan E. Markham -- Analysis of defense signals in Arabidopsis thaliana leaves by ultra-performance liquid chromatography/tandem mass spectrometry : jasmonates, salicylic acid, abscisic acid / Nadja Stingl [and others] -- Analysis of fatty acid amide hydrolase activity in plants / Sang-Chul Kim, Lionel Faure, and Kent D. Chapman -- Ionization behavior of polyphosphoinositides determined via the preparation of pH titration curves using solid-state 31P NMR / Zachary T. Graber and Edgar E. Kooijman -- Phosphatidylinositol synthase activity from plant membrane fractions and from E. coli- expressed recombinant peptides / Sylvie Collin and Françoise Cochet -- Phosphatidylinositol 4-kinase and phosphatidylinositol 4-phosphate 5-kinase assays / Yang Ju Im [and others] -- Assaying inositol and phosphoinositide phosphatase enzymes / Janet L. Donahue, Mustafa Ercetin, and Glenda E. Gillaspy -- Determination of phospholipase C activity in vitro / S.M. Teresa Hernández-Sotomayor and J. Armando Muñoz-Sanchez -- Assaying nonspecific phospholipase C activity / P řemysl Pejchar, Günther F.E. Scherer, and Jan Martinec -- Assaying different types of plant phospholipase D activities in vitro / Kirk L. Pappan and Xuemin Wang -- Measuring PLD activity in vivo / Teun Munnik and Ana M. Laxalt -- Assaying plant phosphatidic acid phosphatase activity / Yuki Nakamura -- Assay of phospholipase A activity / Michael Heinze and Werner Roos -- Lipid-binding analysis using a fat blot assay / Teun Munnik and Magdalena Wierzchowiecka -- Liposome-binding assays to assess specificity and affinity of phospholipid-protein interactions / Magdalena M. Julkowska, Johanna M. Rankenberg, and Christa Testerink -- Lipid affinity beads : from identifying new lipid binding proteins to assessing their binding properties / Fionn McLoughlin and Christa Testerink -- Using genetically encoded fluorescent reporters to image lipid signalling in living plants / Joop E.M. Vermeer and Teun Munnik -- Imaging changes in cytoplasmic calcium using the yellow cameleon 3.6 biosensor and confocal microscopy / Sarah J. Swanson and Simon Gilroy Summary As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied. These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals Analysis Life sciences Botany Plant Sciences plantkunde planten plants lipiden lipids signaaltransductie signal transduction plantencelbiologie plant cell biology Plant Cell Biology Plantencelbiologie Bibliography Includes bibliographical references and index Notes English Subject Plant cellular signal transduction -- Laboratory manuals Plant lipids -- Laboratory manuals Metabolism. Physical sciences. Plants. Botany. Chemistry. Lipids. Lipids -- Metabolism. Eukaryota Metabolism Publication Formats Natural Science Disciplines Chemicals and Drugs Organisms Publication Characteristics Disciplines and Occupations Phenomena and Processes Laboratory Manuals Plants Chemistry Lipids Lipid Metabolism metabolism (biological concept) physical sciences. vegetation. chemistry. lipid. Plants Physical sciences Metabolism Lipids -- Metabolism Lipids Chemistry Botany Plant cellular signal transduction Plant lipids Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Munnik, Teun. Heilmann, Ingo. ISBN 9781627034012 1627034013 1627034005 9781627034005

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