Title Plant metabolomics : methods and protocols / edited by Nigel W. Hardy, Robert D. Hall

Published New York : Humana Press, ©2012

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Description 1 online resource (xiii, 340 pages) Series Methods in molecular biology, 1064-3745 ; 860 Methods in molecular biology ; 860. Contents Practical applications of metabolomics in plant biology / Robert D. Hall and Nigel W. Hardy -- Part I Material preparation -- Aspects of experimental design for plant metabolomics experiments and guidelines for growth of plant material / Yves Gibon and Dominique Rolin -- Separating the inseparable: the metabolomic analysis of plant-pathogen interactions / J. William Allwood [and others] -- Precautions for harvest, sampling, storage, and transport of crop plant metabolomics samples / Benoît Biais [and others] -- Tissue preparation using arabidopsis / Aimee M. Llewellyn [and others] -- Part II Chemical analysis approaches -- Solid phase micro-extraction GC-MS analysis of natural volatile components in melon and rice / Harrie A. Verhoeven [and others] -- Profiling primary metabolites of tomato fruit with gas chromatography/mass spectrometry / Sonia Osorio, Phuc Thi Do, and Alisdair R. Fernie -- High-performance liquid chromatography-mass spectrometry analysis of plant metabolites in brassicaceae / Ric C.H. De Vos, Bert Schipper, and Robert D. Hall -- UPLC-MS-based metabolite analysis in tomato / Ilana Rogachev and Asaph Aharoni -- High precision measurement and fragmentation analysis for metabolite identification / Madalina Oppermann [and others] -- Fourier transform ion cyclotron resonance mass-spectrometry for plant metabolite profiling and metabolite identification / J. William Allwood [and others] -- Combined NMR and flow injection ESI-MS for brassicaceae metabolomics / John M. Baker, Jane L. Ward, and Michael H. Beale -- ICP-MS and LC-ICP-MS for analysis of trace elements content and speciation in cereal grains / D.P. Persson [and others] -- The use of genomics and metabolomics methods to quantify fungal endosymbionts and alkaloids in grasses / Susanne Rasmussen [and others] -- Part III Data analysis -- Data (pre- )processing of nominal and accurate mass LC-MS or GC-MS data using MetAlign / Arjen Lommen -- TagFinder: preprocessing software for the fingerprinting and the profiling of gas chromatography-mass spectrometry based metabolome analyses / Alexander Luedemann [and others] -- Chemical identification strategies using liquid chromatography-photodiode array-solid phase extraction-nuclear magnetic resonance/mass spectrometry / Sofia Moco and Jacques Vervoort -- A strategy for selecting data mining techniques in metabolomics / Ahmed Hmaidan BaniMustafa and Nigel W. Hardy Summary Estimation of the metabolite complement of plant material involves a wide range of techniques and technologies and that breadth continues to increase. Metabolomics research typically involves multiple sites for material preparation and analysis and most investigations are "high throughput", meaning that chemical analysis of sample sets are inevitably carried out over an extended period of time. In, Plant Metabolomics: Methods and Protocols expert researchers in the field detail many of the stages which are now commonly used to study plant metabolomics workflow. Stages of this workflow, up to and including the statistical analysis, accurate and detailed collection of meta-data are also essential for good process management, to satisfy reporting requirements and to ensure wider interpretability and reuse results. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Through and intuitive Plant Metabolomics: Methods and Protocols, seeks to aid scientists in the further study of the methods for all the stages of the plant metabolomics workflow Analysis Life sciences Biology -- Data processing Botany Plant physiology Plant Sciences Computer Appl. in Life Sciences planten plants metabolomica metabolomics plantenfysiologie methodologie methodology protocollen protocols Metabolomics Notes Includes index Bibliography Includes bibliographical references and index Notes English Subject Plant metabolites -- Laboratory manuals Plants -- Metabolism -- Laboratory manuals Biochemistry. Computational biology. Bioinformatics. Life sciences. Chemistry. Biology. Physical sciences. Botany. Plants. Biochemistry Computational Biology Eukaryota Biological Science Disciplines Chemistry Biology Organisms Natural Science Disciplines Disciplines and Occupations Metabolomics Plants biochemistry. biological sciences. chemistry. biology. physical sciences. vegetation. Plants Physical sciences Life sciences Computational biology Chemistry Botany Biology Bioinformatics Biochemistry Plant metabolites Plants -- Metabolism Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Hardy, Nigel W Hall, Robert D. (Robert David), 1958- ISBN 9781617795947 1617795941 1617795933 9781617795930

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