Title Recombinant protein production in yeast : methods and protocols / edited by Roslyn M. Bill

Published New York : Humana Press, ©2012

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Description 1 online resource (xii, 248 pages) Series Methods in molecular biology, 1064-3745 ; 866 Methods in molecular biology (Clifton, N.J.) ; v. 866. 1064-3745 Contents Optimising yeast as a host for recombinant protein production (review) / Nicklas Bonander and Roslyn M. Bill -- Which yeast species shall I choose? : Saccharomyces cerevisiae versus Pichia pastoris (review) / Richard A.J. Darby [and others] -- Preparation of Pichia pastoris expression plasmids / Christel Logez [and others] -- Preparation of Saccharomyces cerevisiae expression plasmids / David Drew and Hyun Kim -- Codon optimisation for heterologous gene expression in yeast / Kristina Hedfalk -- Yeast transformation to generate high-yielding clones / Mohammed Jamshad and Richard A.J. Darby -- Screening for high-yielding Pichia pastoris clones : the production of G protein-coupled receptors as a case study / Shweta Singh [and others] -- Screening for high-yielding Saccharomyces cerevisiae clones: using a green fluorescent protein fusion strategy in the production of membrane proteins / David Drew and Hyun Kim -- The effect of antifoam addition on protein production yields / Sarah J. Routledge and Roslyn M. Bill -- Setting up a bioreactor for recombinant protein production in yeast / Sarah J. Routlledge and Michelle Clare -- The implementation of a design of experiments strategy to increase recombinant protein yields in yeast (review) / Nagamani Bora [and others] -- Online analysis and process control in recombinant protein production (review) / Shane M. Palmer and Edmund R.S. Kunji -- Monitoring the biomass accumulation of recombinant yeast cultures : offline estimations of dry cell mass and cell counts / Shane M. Palmer and Edmund R.S. Kunji -- Online monitoring of biomass accumulation in recombinant yeast cultures / Shane M. Palmer and Edmund R.S. Kunji -- Optimising Pichia pastoris induction / Zharain Bawa and Richard A.J. Darby -- Optimizing Saccharomyces cerevisiae induction regimes / David Drew and Hyun Kim -- Large-scale production of membrane proteins in Pichia pastoris : the production of G protein-coupled receptors as a case study / Shweta Singh [and others] -- Large-scale production of secreted proteins in Pichia pastoris / Nagamani Bora -- Disruption of yeast cells to isolate recombinant proteins / Mohammed Jamshad and Richard A.J. Darby Summary In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host Analysis Medicine Biomedicine biomedische wetenschappen humane genetica human genetics Medicine (General) Geneeskunde (algemeen) Notes Includes index Bibliography Includes bibliographical references and index Notes English Subject Recombinant proteins -- Laboratory manuals Yeast fungi -- Biotechnology -- Laboratory manuals Proteins. Saccharomyces. Endomycetales. Ascomycetes. Yeast fungi. Pichia. Recombinant proteins. Saccharomyces cerevisiae. Fungi Proteins Publication Formats Saccharomyces Saccharomycetales Publication Characteristics Ascomycota Eukaryota Amino Acids, Peptides, and Proteins Chemicals and Drugs Organisms Yeasts Pichia Recombinant Proteins Saccharomyces cerevisiae Laboratory Manuals protein. Yeast fungi Saccharomyces cerevisiae Saccharomyces Proteins Pichia Endomycetales Ascomycetes Recombinant proteins Yeast fungi -- Biotechnology Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Bill, Roslyn M., 1967- ISBN 9781617797705 1617797707 1617797693 9781617797699

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