Title Hydrocarbon and lipid microbiology protocols : synthetic and systems biology -- tools / Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors

Published Berlin : Springer, 2016

Click on the following:
Springer eBooks

Copies

Description 1 online resource (x, 174 pages) Series Springer protocols handbooks, 1949-2448 Springer protocols (Series) 1949-2448 Contents Introduction -- Protocol for the standardisation of transcriptional measurements -- Uracil-excision for assembly of complex pathways -- Quantitative physiology approaches to understand and optimize reducing power availability in environmental bacteria -- Design of orthogonal pairs for protein translation: selection systems for genetically encoding noncanonical amino acids in E. coli -- Phenome-ing microbes -- Systems biology tools for methylotrophs -- Protocols for probing genome architecture of regulatory networks in hydrocarbon and lipid microorganisms -- A practical protocol for genome-scale metabolic reconstruction -- Computer-guided metabolic engineering -- Improving biocontainment with synthetic biology: beyond physical containment Summary This Volume presents generic protocols for wet experimental and computer-based systems and synthetic biology approaches relevant to the field of hydrocarbon and lipid microbiology. It complements a second Volume that describes protocols for systems and synthetic biology applications. The wet experimental tools presented in this Volume include protocols for the standardisation of transcriptional measurements, application of uracil excision-based DNA editing for, inter alia, multi-gene assembly, the use of fluxomics to optimise "reducing power availability", and the incorporation of non-canonical amino acids into proteins for optimisation of activities. Phenome-ing microbes, using a combination of RNA-seq and bioinformatic algorithms, is presented, as is an illustration, using methylotrophs as an example, of how the different key omics approaches constitute a pipeline for functional analysis, acquisition of a systems overview, and metabolic optimisation. Complementary computational tools that are presented include protocols for probing the genome architecture of regulatory networks, genome-scale metabolic reconstruction, and bioinformatic approaches to guide metabolic engineering. The Volume also includes an overview of how synthetic biology approaches can be used to improve biocontainment. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes Bibliography Includes bibliographical references Notes Online resource; title from PDF title page (SpringerLink, viewed June 22, 2016) Subject Lipids -- Laboratory manuals Lipids -- Biotechnology -- Laboratory manuals Hydrocarbons -- Laboratory manuals Hydrocarbons. Lipids. Hydrocarbons Lipids lipid. Hydrocarbons Lipids Lipids -- Biotechnology Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author McGenity, Terry J., editor Timmis, K. N., editor. Nogales, Balbina, editor ISBN 9783662504321 3662504324

  Permalink