| Description |
1 online resource (xi, 375 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; v. 1182 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1182. 1064-3745
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| Contents |
PART I. STRUCTURAL RNA MAPPING -- 1. Full-Length Characterization of Transcribed Genomic Region / Marc R. Reboll, M. Lucrecia Alvarez, and Mahtab Nourbakhsh -- 2. Rapid Mapping of RNA 3ʹ and 5ʹ Ends / Victoria Zismann and Mahtab Nourbakhsh -- 3. Single Nucleotide Mapping of RNA 5ʹ and 3ʹ Ends / Mahtab Nourbakhsh -- 4. Analysis of RNA Secondary Structure / Mahtab Nourbakhsh -- 5. Tertiary Structure Mapping of the Pri-miRNA miR-17̃92 / Steven G. Chaulk and Richard P. Fahlman -- 6. In Situ Hybridization Detection of miRNA Using LNA Oligonucleotides / Stefania Cotta Doné and Olga Beltcheva -- 7. Quantification of miRNAs by a Simple and Specific qPCR Method / Susanna Cirera and Peter K. Busk -- 8. RNA Isolation for Small RNA Next-Generation Sequencing from Acellular Biofluids / Kasandra L. Burgos and Kendall Van Keuren-Jensen -- 9. Sequencing Small RNA: Introduction and Data Analysis Fundamentals / Jai Prakash Mehta -- 10. Measuring Expression Levels of Small Regulatory RNA Molecules from Body Fluids and Formalin-Fixed, Paraffin-Embedded Samples / Adrienn Gyongyosi, Otto Docs, Zsolt Czimmerer, Laszlo Orosz, Attila Horvath, Olga Török, Gabor Mehes, Laszlo Nagy, and Balint L. Balint -- 11. MicroRNA Profiling in Plasma or Serum Using Quantitative RT-PCR / Marina C. Costa, Ana Lúcia Leitão, and Francisco J. Enguita -- 12. MicroRNA Profiling of Exosomes Isolated from Biofluids and Conditioned Media / Sweta Rani -- 13. Isolation of Urinary Exosomes for RNA Biomarker Discovery -- Using a Simple, Fast, and Highly Scalable Method / M. Lucrecia Alvarez |
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PART II. FUNCTIONAL RNA MAPPING -- 14. Identification of Actively Translated mRNAs / Marc R. Reboll and Mahtab Nourbakhsh -- 15. Mapping of Internal Ribosome Entry Sites (IRES) / Sarah Mehrtens and Marc R. Reboll -- 16. Mapping of Protein Binding RNA Elements / Marc R. Reboll -- 17. Purification of RNA-Binding Proteins / Birgit Ritter and Marc R. Reboll -- 18. De Novo Approach to Classify Protein-Coding and Noncoding Transcripts Based on Sequence Composition / Haitao Luo, Dechao Bu, Liang Sun, Runsheng Chen, and Yi Zhao -- 19. Computational Methods to Predict Long Noncoding RNA Functions -- Based on Co-expression Network / Yi Zhao, Haitao Luo, Xiaowei Chen, Yi Xiao, and Runsheng Chen -- 20. MicroRNA Biogenesis: Dicing Assay / Carlos A. Melo and Sonia A. Melo -- 21. Faster Experimental Validation of microRNA Targets Using Cold Fusion Cloning and a Dual Firefly-Renilla Luciferase Reporter Assay / M. Lucrecia Alvarez -- 22. Experimental Validation of Predicted Mammalian MicroRNAs of Mirtron Origin / Anita Schamberger and Tamás I. Orbán -- 23. A Guide for miRNA Target Prediction and Analysis Using Web-Based Applications / Ana Lúcia Leitão, Marina C. Costa, and Francisco J. Enguita -- 24. Tapping MicroRNA Regulation Networks Through Integrated Analysis of MicroRNA-mRNA High-Throughput Profiles / Anthony D. Saleh and Hui Cheng -- 25. miRWalk Database for miRNA-Target Interactions / Harsh Dweep, Norbert Gretz, and Carsten Sticht -- 26. A Schematic Workflow for Collecting Information About the Interaction Between Copy Number Variants and MicroRNAs Using Existing Resources / Harsh Dweep, Norbert Gretz, and Kyriakos Felekkis -- 27. SYBR® Green and TaqMan® Quantitative PCR Arrays: Expression Profile of Genes Relevant to a Pathway or a Disease State / M. Lucrecia Alvarez and Stefania Cotta Doné -- 28. Comprehensive Meta-analysis of MicroRNA Expression Using a Robust Rank Aggregation Approach / Urmo Võsa, Raivo Kolde, Jaak Vilo, Andres Metspalu, and Tarmo Annilo |
| Summary |
Annotation In "RNA Mapping- Methods and Protocols" expert researchers in the field detail many of the methods which are now commonly used to study RNA. These include protocols for the consequence of the emerging interest in the characterization of cellular RNAs urged by their potential use as diagnostic biomarkers or therapeutic targets. In particular, the biological relevance of microRNAs in human physiology and disease development is highlighted in the 16 chapters focused on methods for their physical and functional mapping. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, "RNA Mapping- Methods and Protocols" provides instruction and inspiration for scientists who are facing the challenges of the discovery and/or functional characterization of RNA molecules for a wide variety of applications ranging from novel biomedical diagnostics to therapeutics and biomaterials |
| Analysis |
biomedische wetenschappen |
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biomedicine |
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moleculaire genetica |
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molecular genetics |
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humane genetica |
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human genetics |
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Medicine (General) |
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Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed August 4, 2014) |
| Subject |
Gene mapping -- Laboratory manuals
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Chromosome Mapping -- methods
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RNA -- genetics
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Gene mapping
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Alvarez, M. Lucrecia, editor
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Nourbakhsh, Mahtab, editor
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| ISBN |
9781493910625 |
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1493910620 |
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1493910612 |
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9781493910618 |
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