Title Arabidopsis protocols / edited by Jose J. Sanchez-Serrano and Julio Salinas

Published New York : Humana Press : Springer, ©2014

Click on the following:
Springer eBooks

Copies

Description 1 online resource (xvi, 726 pages) : illustrations (some color) Series Methods in molecular biology ; v. 1062 Methods in molecular biology (Clifton, N.J.) ; v. 1062. 1064-3745 Contents Handling Arabidopsis plants : growth, preservation of seeds, transformation, and genetic crosses / Luz Rivero [and others] -- Using arabidopsis-related model species (ARMS) : growth, genetic transformation, and comparative genomics / Giorgia Batelli [and others] -- Growing arabidopsis in vitro : cell suspensions, in vitro culture, and regeneration / Bronwyn J. Barkla, Rosario Vera-Estrella, and Omar Pantoja -- Arabidopsis database and stock resources / Donghui Li [and others] -- Bioinformatic tools in Arabidopsis research / Miguel de Lucas, Nicholas J. Provart, and Siobhan M. Brady -- Exploiting natural variation in Arabidopsis / Johanna A. Molenaar and Joost J.B. Keurentjes -- Grafting in arabidopsis / Katherine Bainbridge [and others] -- Agrobacterium tumefaciens-mediated transient transformation of Arabidopsis thaliana leaves / Silvina Mangano, Cintia Daniela Gonzalez, and Silvana Petruccelli -- iTILLING : personalized mutation screening / Susan M. Bush and Patrick J. Krysan -- Tailor-Made Mutations in Arabidopsis Using Zinc Finger Nucleases / Yiping Qi [and others] -- Use of artificial microRNA technology to control gene expression in Arabidopsis thaliana / Andrew L. Eamens, Marcus McHale, and Peter M. Waterhouse -- Generation and identification of Arabidopsis EMS mutants / Li-Jia Qu and Genji Qin -- Generation and characterization of arabidopsis T-DNA insertion mutants / Li-Jia Qu and Genji Qin -- Identification of EMS-induced causal mutations in Arabidopsis thaliana by next-generation sequencing / Naoyuki Uchida [and others] -- Arabidopsis transformation with large bacterial artificial chromosomes / Jose M. Alonso and Anna N. Stepanova -- Global DNA methylation analysis using methyl-sensitive amplification polymorphism (MSAP) / Mahmoud W. Yaish, Mingsheng Peng, and Steven J. Rothstein -- Next-generation mapping of genetic mutations using bulk population sequencing / Ryan S. Austin [and others] -- Chemical fingerprinting of arabidopsis using fourier transform infrared (FT-IR) spectroscopic approaches / András Gorzsás and Björn Sundberg -- Pipeline for ¹⁵N metabolic labeling and phosphoproteome analysis in Aarabidopsis thaliana / Benjamin B. Minkoff, Heather L. Burch, and Michael R. Sussman -- Gene expression profiling using DNA microarrays / Kyonoshin Maruyama, Kazuko Yamaguchi-Shinozaki, and Kazuo Shinozaki -- Forward chemical genetic screening / Hyunmo Choi [and others] -- Highly reproducible ChIP-on-chip analysis to identify genome-wide protein binding and chromatin status in Arabidopsis thaliana / Jong-Myong Kim [and others] -- Fluorescence microscopy / Sébastien Peter, Klaus Harter, and Frank Schleifenbaum -- Immunocytochemical fluorescent in situ visualization of proteins in Arabidopsis / Yohann Boutté and Markus Grebe -- High-pressure freezing and freeze substitution of arabidopsis for electron microscopy / Jotham R. Austin II -- Applications of fluorescent marker proteins in plant cell biology / Michael R. Blatt and Christopher Grefen -- Flow cytometry and sorting in arabidopsis / David W. Galbraith -- Live imaging of Arabidopsis development / Daniel von Wangenheim [and others] -- Arabidopsis Organelle Isolation and Characterization / Nicolas L. Taylor, Elke Ströher, and A. Harvey Millar -- Analysis of subcellular metabolite distributions within Arabidopsis thaliana leaf tissue : a primer for subcellular metabolomics / Stephan Krueger [and others] -- Hormone profiling / Gaetan Glauser, Armelle Vallat, and Dirk Balmer -- Purification of protein complexes and characterization of protein-protein interactions / Kirby N. Swatek, Chris B. Lee, and Jay J. Thelen -- Protein fragment bimolecular fluorescence complementation analyses for the in vivo study of protein-protein interactions and cellular protein complex localizations / Rainer Waadt [and others] -- Split-ubiquitin system for the analysis of three-component interactions / Christopher Grefen -- RNA-binding protein immunoprecipitation from whole-cell extracts / Tino Köster and Dorothee Staiger -- High-throughput analysis of protein-DNA binding affinity / José M. Franco-Zorrilla and Roberto Solano Summary Arabidopsis Protocols, Third Edition compiles some of the most recent methodologies developed to exploit the Arabidopsis genome These methodologies cover from the guided access to public resources, to genetic, cell biology, biochemical and physiological techniques, including both those that are widely used as well as those novel techniques likely to open up new avenues of knowledge in the future. In addition, considering the recent unparalleled progress of the "omics" tools in Arabidopsis, leading experts have contributed sections on genome, transcriptome, proteome, metabolome and other whole-system approaches Arabidopsis thaliana is acknowledged as the most important plant model system by the scientific community and Arabidopsis research has fundamentally influenced our understanding of the basic biology and ecology of plants Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls Authoritative and easily accessible, Arabidopsis Protocols, Third Edition seeks to serve both experienced researchers and beginners with its detailed methodologies on this burgeoning scientific field. Analysis arabidopsis thaliana genexpressie gene expression genomica genomics plantengenetica plant genetics genen genes genetische variatie genetic variation moleculaire biologie molecular biology genkartering gene mapping protocollen protocols Plant Molecular Biology Moleculaire plantenbiologie Bibliography Includes bibliographical references and index Subject Arabidopsis -- Laboratory manuals Arabidopsis -- Molecular aspects Arabidopsis. Arabidopsis Plant Preparations Plant Structures Arabidopsis Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Sánchez-Serrano, Jose J. Salinas, Julio. ISBN 9781627035804 162703580X 1627035796 9781627035798

  Permalink