| Description |
1 online resource (xi, 406 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1361 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1361. 1064-3745
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| Contents |
Using RNA-seq for analysis of differential gene expression in fungal species / Can Wang [and others] -- Enhancing structural annotation of yeast genomes with RNA-Seq data / Hugo Devillers, Nicolas Morin, and Cécile Neuvéglise -- Pathogen gene expression profiling during infection using a nanostring nCounter platform / Wenjie Xu [and others] -- Comparative transcriptomics in yeasts / Dawn A. Thompson -- Mapping the transcriptome-wide landscape of RBP binding sites using gPAR-CLIP-seq : experimental procedures / Ting Han and John K. Kim -- Mapping the transcriptome-wide landscape of RBP binding sites using gPAR-CLIP-seq : bioinformatic analysis / Mallory A. Freeberg and John K. Kim -- Translation analysis at the genome scale by ribosome profiling / Agnès Baudin-Baillieu [and others] -- Biotin-genomic run-on (Bio-GRO) : a high-resolution method for the analysis of nascent transcription in yeast / Antonio Jordán-Pla [and others] -- Genome-wide probing of RNA structures in vitro using nucleases and deep sequencing / Yue Wan [and others] -- Genome-wide chromatin immunoprecipitation in Candida albicans and other yeasts / Matthew B. Lohse [and others] -- ChIPseq in yeast species : from chromatin immunoprecipitation to high-throughput sequencing and bioinformatics data analyses / Gaëlle Lelandais, Corinne Blugeon, and Jawad Merhej -- Systematic determination of transcription factor DNA- binding specificities in yeast / Lourdes Peña-Castillo and Gwenael Badis -- Generation and analysis of chromosomal contact maps of yeast species / Axel Cournac [and others] -- Versatile procedure to generate genome-wide spatiotemporal program of replication in yeast species / Nicolas Agier and Gilles Fischer -- Single-step affinity purification (ssAP) and mass spectrometry of macromolecular complexes in the yeast S. cerevisiae / Christian Trahan, Lisbeth-Carolina Aguilar, and Marlene Oeffinger -- Label-free quantitative proteomics in yeast / Thibaut Léger [and others] -- Profiling of yeast lipids by shotgun lipidomics / Christian Klose and Kirill Tarasov -- Identification of links between cellular pathways by genetic interaction mapping (GIM) / Christophe Malabat and Cosmin Saveanu -- On the mapping of epistatic genetic interactions in natural isolates : combining classical genetics and genomics / Jing Hou and Joseph Schacherer -- Experimental evolution and resequencing analysis of yeast / Celia Payen and Maitreya J. Dunham -- Reconstruction and analysis of the evolution of modular transcriptional regulatory programs using arboretum / Sara A. Knaack, Dawn A. Thompson, and Sushmita Roy -- Predicting gene and genomic regulation in Saccharomyces cerevisiae, using the YEASTRACT database : a step-by-step guided analysis / Miguel C. Teixeira, Pedro T. Monteiro, and Isabel Sá-Correia |
| Summary |
This volume provides a collection of protocols for the study of DNA-DNA contact maps, replication profiles, transcription rates, RNA secondary structures, protein-RNA interactions, ribosome profiling and quantitative proteomes and metabolomes. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Functional Genomics: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Saccharomyces cerevisiae -- Genetics
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Molecular genetics -- Technique
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Genetics -- Technique.
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Medical laboratory technology.
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Saccharomyces cerevisiae -- genetics
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Genetic Techniques
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Genomics -- methods
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Clinical Laboratory Techniques
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Proteomics -- methods
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Medical genetics.
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Microbiology (non-medical)
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Medical -- Genetics.
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Science -- Life Sciences -- Biology -- Microbiology.
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Medical laboratory technology
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Genetics -- Technique
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Molecular genetics -- Technique
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Saccharomyces cerevisiae -- Genetics
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Devaux, Frédéric, 1972- editor.
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| ISBN |
9781493930791 |
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1493930796 |
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